Study on the Stability of Antibiotics, Pesticides and Drugs in Water by Using a Straightforward Procedure Applying HPLC-Mass Spectrometric Determination for Analytical Purposes
The stability of analytes is a critical point in chemical analysis, especially in the field of trace levels residue analysis. Nowadays, due to advances in analytical technology and in separation sciences, the analyses of water have been improved. Unfortunately, in this context, one of the most criti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Separations |
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| Online Access: | https://www.mdpi.com/2297-8739/8/10/179 |
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| author | Salvatore Barreca Carola Forni Luisa Colzani Laura Clerici Daniela Daverio Pierluisa Dellavedova |
| author_facet | Salvatore Barreca Carola Forni Luisa Colzani Laura Clerici Daniela Daverio Pierluisa Dellavedova |
| author_sort | Salvatore Barreca |
| collection | DOAJ |
| description | The stability of analytes is a critical point in chemical analysis, especially in the field of trace levels residue analysis. Nowadays, due to advances in analytical technology and in separation sciences, the analyses of water have been improved. Unfortunately, in this context, one of the most critical issues in water analysis include compound stability from sampling station to laboratory procedures. This study was carried out to explore the stability of several compounds in water from sampling to analysis concerning analytes reported in implementing decision 2018/840—Watch List. During method development and validation, the stability of compounds was investigated to detect the best operating conditions concerning sampling, extraction and analysis. In this paper, we report a study on the stability of antibiotics, pesticides and drugs in water determined using a straight-forward procedure applying mass spectrometric detection for analytical purposes. The laboratory tests were performed in Milli-Q water and surface water by analyzing samples through direct injection, solvent mixture (Water/ACN) and solid phase extraction system from time 0 to 168 h. All the analytes of the WL are stable in aqueous solutions with the addition of at least 25% ACN even after 168 h, and the analytes have shown a matrix effect on recovery of some analytes such as Famoxadone from sampling results (recovery in surface water 72%). For all the analytes investigated, recoveries were between 70 and 130% by using SPE procedures before UHPLC-MS/MS analysis, which is in good agreement with method validation procedures. |
| first_indexed | 2024-03-10T06:12:09Z |
| format | Article |
| id | doaj.art-36cbfc7b62724b758150a38ad3c1a89f |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-10T06:12:09Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-36cbfc7b62724b758150a38ad3c1a89f2023-11-22T20:00:14ZengMDPI AGSeparations2297-87392021-10-0181017910.3390/separations8100179Study on the Stability of Antibiotics, Pesticides and Drugs in Water by Using a Straightforward Procedure Applying HPLC-Mass Spectrometric Determination for Analytical PurposesSalvatore Barreca0Carola Forni1Luisa Colzani2Laura Clerici3Daniela Daverio4Pierluisa Dellavedova5Agenzia Regionale per la Protezione dell’Ambiente della Lombardia (ARPA Lombardia), U.O.C. Laboratorio Regionale Area Ovest U.O.S. Laboratorio Tematico Trasversale Area Ovest 1 Sede Laboratoristica di Monza e Brianza via Solferino n 16, 20900 Monza, ItalyAgenzia Regionale per la Protezione dell’Ambiente della Lombardia (ARPA Lombardia), U.O.C. Laboratorio Regionale Area Ovest U.O.S. Laboratorio Tematico Trasversale Area Ovest 1 Sede Laboratoristica di Monza e Brianza via Solferino n 16, 20900 Monza, ItalyAgenzia Regionale per la Protezione dell’Ambiente della Lombardia (ARPA Lombardia), U.O.C. Laboratorio Regionale Area Ovest U.O.S. Laboratorio Tematico Trasversale Area Ovest 1 Sede Laboratoristica di Monza e Brianza via Solferino n 16, 20900 Monza, ItalyAgenzia Regionale per la Protezione dell’Ambiente della Lombardia (ARPA Lombardia), U.O.C. Laboratorio Regionale Area Ovest U.O.S. Laboratorio Tematico Trasversale Area Ovest 1 Sede Laboratoristica di Monza e Brianza via Solferino n 16, 20900 Monza, ItalyAgenzia Regionale per la Protezione dell’Ambiente della Lombardia (ARPA Lombardia), U.O.C. Laboratorio Regionale Area Ovest U.O.S. Laboratorio Tematico Trasversale Area Ovest 1 Sede Laboratoristica di Monza e Brianza via Solferino n 16, 20900 Monza, ItalyAgenzia Regionale per la Protezione dell’Ambiente della Lombardia (ARPA Lombardia), U.O.C. Laboratorio Regionale Area Ovest U.O.S. Laboratorio Tematico Trasversale Area Ovest 1 Sede Laboratoristica di Monza e Brianza via Solferino n 16, 20900 Monza, ItalyThe stability of analytes is a critical point in chemical analysis, especially in the field of trace levels residue analysis. Nowadays, due to advances in analytical technology and in separation sciences, the analyses of water have been improved. Unfortunately, in this context, one of the most critical issues in water analysis include compound stability from sampling station to laboratory procedures. This study was carried out to explore the stability of several compounds in water from sampling to analysis concerning analytes reported in implementing decision 2018/840—Watch List. During method development and validation, the stability of compounds was investigated to detect the best operating conditions concerning sampling, extraction and analysis. In this paper, we report a study on the stability of antibiotics, pesticides and drugs in water determined using a straight-forward procedure applying mass spectrometric detection for analytical purposes. The laboratory tests were performed in Milli-Q water and surface water by analyzing samples through direct injection, solvent mixture (Water/ACN) and solid phase extraction system from time 0 to 168 h. All the analytes of the WL are stable in aqueous solutions with the addition of at least 25% ACN even after 168 h, and the analytes have shown a matrix effect on recovery of some analytes such as Famoxadone from sampling results (recovery in surface water 72%). For all the analytes investigated, recoveries were between 70 and 130% by using SPE procedures before UHPLC-MS/MS analysis, which is in good agreement with method validation procedures.https://www.mdpi.com/2297-8739/8/10/179stabilityEU Watch listultra-high-performance liquid chromatographyantibiotic determination |
| spellingShingle | Salvatore Barreca Carola Forni Luisa Colzani Laura Clerici Daniela Daverio Pierluisa Dellavedova Study on the Stability of Antibiotics, Pesticides and Drugs in Water by Using a Straightforward Procedure Applying HPLC-Mass Spectrometric Determination for Analytical Purposes Separations stability EU Watch list ultra-high-performance liquid chromatography antibiotic determination |
| title | Study on the Stability of Antibiotics, Pesticides and Drugs in Water by Using a Straightforward Procedure Applying HPLC-Mass Spectrometric Determination for Analytical Purposes |
| title_full | Study on the Stability of Antibiotics, Pesticides and Drugs in Water by Using a Straightforward Procedure Applying HPLC-Mass Spectrometric Determination for Analytical Purposes |
| title_fullStr | Study on the Stability of Antibiotics, Pesticides and Drugs in Water by Using a Straightforward Procedure Applying HPLC-Mass Spectrometric Determination for Analytical Purposes |
| title_full_unstemmed | Study on the Stability of Antibiotics, Pesticides and Drugs in Water by Using a Straightforward Procedure Applying HPLC-Mass Spectrometric Determination for Analytical Purposes |
| title_short | Study on the Stability of Antibiotics, Pesticides and Drugs in Water by Using a Straightforward Procedure Applying HPLC-Mass Spectrometric Determination for Analytical Purposes |
| title_sort | study on the stability of antibiotics pesticides and drugs in water by using a straightforward procedure applying hplc mass spectrometric determination for analytical purposes |
| topic | stability EU Watch list ultra-high-performance liquid chromatography antibiotic determination |
| url | https://www.mdpi.com/2297-8739/8/10/179 |
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