Expression of MRJP3 and HSP70 mRNA Levels in <i>Apis mellifera</i> L. Workers after Dietary Supplementation with Proteins, Prebiotics, and Probiotics
Royal jelly is an essential substance for the development of bees from larval to adult stages. Studies have identified a group of key proteins in royal jelly, denominated major royal jelly proteins (MRJPs). The group currently consists of nine proteins (MRJP1–MRJP9), with MRJP1 being the most abunda...
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MDPI AG
2022-06-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/7/571 |
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| author | Maria Carolina Paleari Varjão Oliveira Eloisa Magalhaes Pereira Maria Josiane Sereia Érica Gomes Lima Breno Gabriel Silva Vagner Alencar Arnaut Toledo Maria Claudia Colla Ruvolo-Takasusuki |
| author_facet | Maria Carolina Paleari Varjão Oliveira Eloisa Magalhaes Pereira Maria Josiane Sereia Érica Gomes Lima Breno Gabriel Silva Vagner Alencar Arnaut Toledo Maria Claudia Colla Ruvolo-Takasusuki |
| author_sort | Maria Carolina Paleari Varjão Oliveira |
| collection | DOAJ |
| description | Royal jelly is an essential substance for the development of bees from larval to adult stages. Studies have identified a group of key proteins in royal jelly, denominated major royal jelly proteins (MRJPs). The group currently consists of nine proteins (MRJP1–MRJP9), with MRJP1 being the most abundant and MRJP3 being used as a microsatellite marker for the selection of queens with a greater production of royal jelly. The diet of bees is mostly composed of proteins, and supplementing this intake to encourage a higher production of their primary product is important for producers. It is estimated that, by adding probiotic and prebiotic organisms to their diets, the benefits to bees will be even greater, both for their immune systems and primary responses to stress. Circumstances that are adverse compared to those of the natural habitat of bees eventually substantially interfere with bee behavior. Stress situations are modulated by proteins termed heat shock proteins (HSPs). Among these, HSP70 has been shown to exhibit abundance changes whenever bees experience unusual situations of stress. Thus, we sought to supplement <i>A. mellifera</i> bee colony diets with proteins and prebiotic and probiotic components, and to evaluate the expression levels of MRJP3 and HSP70 mRNAs using qRT-PCR. The results revealed that differences in the expression of MRJP3 can be attributed to the different types of feed offered. Significant differences were evident when comparing the expression levels of MRJP3 and HSP70, suggesting that protein supplementation with pre/probiotics promotes positive results in royal jelly synthesis carried out by honey bee nurses. |
| first_indexed | 2024-03-09T13:39:10Z |
| format | Article |
| id | doaj.art-36cc579f95dc4dcb91a6324d5abea716 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T13:39:10Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-36cc579f95dc4dcb91a6324d5abea7162023-11-30T21:08:29ZengMDPI AGInsects2075-44502022-06-0113757110.3390/insects13070571Expression of MRJP3 and HSP70 mRNA Levels in <i>Apis mellifera</i> L. Workers after Dietary Supplementation with Proteins, Prebiotics, and ProbioticsMaria Carolina Paleari Varjão Oliveira0Eloisa Magalhaes Pereira1Maria Josiane Sereia2Érica Gomes Lima3Breno Gabriel Silva4Vagner Alencar Arnaut Toledo5Maria Claudia Colla Ruvolo-Takasusuki6Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, Maringá CEP 87020-900, BrazilDepartment of Biotechnology, Genetics and Cell Biology, State University of Maringá, Maringá CEP 87020-900, BrazilDepartment of Engineering and Food Technology, Federal Technological University of Paraná, Campo Mourão CEP 87301-899, BrazilDepartment of Animal Science, Federal University of Sergipe, Aracaju CEP 49100-000, BrazilDepartment of Exact Sciences, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba CEP 13418-900, BrazilDepartment of Animal Science, State University of Maringá, Maringá CEP 87020-900, BrazilDepartment of Biotechnology, Genetics and Cell Biology, State University of Maringá, Maringá CEP 87020-900, BrazilRoyal jelly is an essential substance for the development of bees from larval to adult stages. Studies have identified a group of key proteins in royal jelly, denominated major royal jelly proteins (MRJPs). The group currently consists of nine proteins (MRJP1–MRJP9), with MRJP1 being the most abundant and MRJP3 being used as a microsatellite marker for the selection of queens with a greater production of royal jelly. The diet of bees is mostly composed of proteins, and supplementing this intake to encourage a higher production of their primary product is important for producers. It is estimated that, by adding probiotic and prebiotic organisms to their diets, the benefits to bees will be even greater, both for their immune systems and primary responses to stress. Circumstances that are adverse compared to those of the natural habitat of bees eventually substantially interfere with bee behavior. Stress situations are modulated by proteins termed heat shock proteins (HSPs). Among these, HSP70 has been shown to exhibit abundance changes whenever bees experience unusual situations of stress. Thus, we sought to supplement <i>A. mellifera</i> bee colony diets with proteins and prebiotic and probiotic components, and to evaluate the expression levels of MRJP3 and HSP70 mRNAs using qRT-PCR. The results revealed that differences in the expression of MRJP3 can be attributed to the different types of feed offered. Significant differences were evident when comparing the expression levels of MRJP3 and HSP70, suggesting that protein supplementation with pre/probiotics promotes positive results in royal jelly synthesis carried out by honey bee nurses.https://www.mdpi.com/2075-4450/13/7/571Africanized honey beenutritiongene expressionheat shock proteinsmajor royal jelly protein-3 |
| spellingShingle | Maria Carolina Paleari Varjão Oliveira Eloisa Magalhaes Pereira Maria Josiane Sereia Érica Gomes Lima Breno Gabriel Silva Vagner Alencar Arnaut Toledo Maria Claudia Colla Ruvolo-Takasusuki Expression of MRJP3 and HSP70 mRNA Levels in <i>Apis mellifera</i> L. Workers after Dietary Supplementation with Proteins, Prebiotics, and Probiotics Insects Africanized honey bee nutrition gene expression heat shock proteins major royal jelly protein-3 |
| title | Expression of MRJP3 and HSP70 mRNA Levels in <i>Apis mellifera</i> L. Workers after Dietary Supplementation with Proteins, Prebiotics, and Probiotics |
| title_full | Expression of MRJP3 and HSP70 mRNA Levels in <i>Apis mellifera</i> L. Workers after Dietary Supplementation with Proteins, Prebiotics, and Probiotics |
| title_fullStr | Expression of MRJP3 and HSP70 mRNA Levels in <i>Apis mellifera</i> L. Workers after Dietary Supplementation with Proteins, Prebiotics, and Probiotics |
| title_full_unstemmed | Expression of MRJP3 and HSP70 mRNA Levels in <i>Apis mellifera</i> L. Workers after Dietary Supplementation with Proteins, Prebiotics, and Probiotics |
| title_short | Expression of MRJP3 and HSP70 mRNA Levels in <i>Apis mellifera</i> L. Workers after Dietary Supplementation with Proteins, Prebiotics, and Probiotics |
| title_sort | expression of mrjp3 and hsp70 mrna levels in i apis mellifera i l workers after dietary supplementation with proteins prebiotics and probiotics |
| topic | Africanized honey bee nutrition gene expression heat shock proteins major royal jelly protein-3 |
| url | https://www.mdpi.com/2075-4450/13/7/571 |
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