Living Bacterial Hydrogels for Accelerated Infected Wound Healing
Abstract Damaged skin cannot prevent harmful bacteria from invading tissues, causing infected wounds and even serious tissue damage. Traditional treatments can not only kill pathogenic bacteria, but also suppress the growth of beneficial bacteria, thus destroying the balance of the damaged skin micr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-12-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202102545 |
| _version_ | 1819100234527014912 |
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| author | Zunzhen Ming Lin Han Meiyu Bao Huanhuan Zhu Sujing Qiang Shaobo Xue Weiwei Liu |
| author_facet | Zunzhen Ming Lin Han Meiyu Bao Huanhuan Zhu Sujing Qiang Shaobo Xue Weiwei Liu |
| author_sort | Zunzhen Ming |
| collection | DOAJ |
| description | Abstract Damaged skin cannot prevent harmful bacteria from invading tissues, causing infected wounds and even serious tissue damage. Traditional treatments can not only kill pathogenic bacteria, but also suppress the growth of beneficial bacteria, thus destroying the balance of the damaged skin microbial ecosystem. Here, a living bacterial hydrogel scaffold is reported that accelerates infected wound healing through beneficial bacteria secreting antibacterial substances. Lactobacillus reuteri, a common probiotic, is encapsulated in hydrogel microspheres by emulsion polymerization and further immobilized in a hydrogel network by covalent cross‐linking of methacrylate‐modified hyaluronic acid. Owing to light‐initiated crosslinking, the hydrogel dressing can be generated in situ at the wound site. This hydrogel scaffold not only protects bacteria from immune system attack, but also prevents bacteria from escaping into the local environment, thus avoiding potential threats. Both in vitro and in vivo experiments show that it has excellent ability against harmful bacteria and anti‐inflammatory capabilities, promoting infected wound closure and new tissue regeneration. This work may open up new avenues for the application of living bacteria in the clinical management of infected wounds. |
| first_indexed | 2024-12-22T00:59:32Z |
| format | Article |
| id | doaj.art-36ccb8856b48481385f88cc1f196ec37 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-12-22T00:59:32Z |
| publishDate | 2021-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-36ccb8856b48481385f88cc1f196ec372022-12-21T18:44:14ZengWileyAdvanced Science2198-38442021-12-01824n/an/a10.1002/advs.202102545Living Bacterial Hydrogels for Accelerated Infected Wound HealingZunzhen Ming0Lin Han1Meiyu Bao2Huanhuan Zhu3Sujing Qiang4Shaobo Xue5Weiwei Liu6Central Laboratory Shanghai Tenth People's Hospital Tongji University 301# Yanchang Middle Road Shanghai 200072 ChinaCentral Laboratory Shanghai Tenth People's Hospital Tongji University 301# Yanchang Middle Road Shanghai 200072 ChinaCentral Laboratory Shanghai Tenth People's Hospital Tongji University 301# Yanchang Middle Road Shanghai 200072 ChinaCentral Laboratory Shanghai Tenth People's Hospital Tongji University 301# Yanchang Middle Road Shanghai 200072 ChinaCentral Laboratory Shanghai Tenth People's Hospital Tongji University 301# Yanchang Middle Road Shanghai 200072 ChinaCentral Laboratory Shanghai Tenth People's Hospital Tongji University 301# Yanchang Middle Road Shanghai 200072 ChinaCentral Laboratory Shanghai Tenth People's Hospital Tongji University 301# Yanchang Middle Road Shanghai 200072 ChinaAbstract Damaged skin cannot prevent harmful bacteria from invading tissues, causing infected wounds and even serious tissue damage. Traditional treatments can not only kill pathogenic bacteria, but also suppress the growth of beneficial bacteria, thus destroying the balance of the damaged skin microbial ecosystem. Here, a living bacterial hydrogel scaffold is reported that accelerates infected wound healing through beneficial bacteria secreting antibacterial substances. Lactobacillus reuteri, a common probiotic, is encapsulated in hydrogel microspheres by emulsion polymerization and further immobilized in a hydrogel network by covalent cross‐linking of methacrylate‐modified hyaluronic acid. Owing to light‐initiated crosslinking, the hydrogel dressing can be generated in situ at the wound site. This hydrogel scaffold not only protects bacteria from immune system attack, but also prevents bacteria from escaping into the local environment, thus avoiding potential threats. Both in vitro and in vivo experiments show that it has excellent ability against harmful bacteria and anti‐inflammatory capabilities, promoting infected wound closure and new tissue regeneration. This work may open up new avenues for the application of living bacteria in the clinical management of infected wounds.https://doi.org/10.1002/advs.202102545antibacterial hydrogelshydrogel microspheresliving bacteriamicrobial competitionwound healing |
| spellingShingle | Zunzhen Ming Lin Han Meiyu Bao Huanhuan Zhu Sujing Qiang Shaobo Xue Weiwei Liu Living Bacterial Hydrogels for Accelerated Infected Wound Healing Advanced Science antibacterial hydrogels hydrogel microspheres living bacteria microbial competition wound healing |
| title | Living Bacterial Hydrogels for Accelerated Infected Wound Healing |
| title_full | Living Bacterial Hydrogels for Accelerated Infected Wound Healing |
| title_fullStr | Living Bacterial Hydrogels for Accelerated Infected Wound Healing |
| title_full_unstemmed | Living Bacterial Hydrogels for Accelerated Infected Wound Healing |
| title_short | Living Bacterial Hydrogels for Accelerated Infected Wound Healing |
| title_sort | living bacterial hydrogels for accelerated infected wound healing |
| topic | antibacterial hydrogels hydrogel microspheres living bacteria microbial competition wound healing |
| url | https://doi.org/10.1002/advs.202102545 |
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