Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization
This article reviews recent developments in microfluidic systems enabling high-throughput characterization of single-cell proteins. Four key perspectives of microfluidic platforms are included in this review: (1) microfluidic fluorescent flow cytometry; (2) droplet based microfluidic flow cytometry;...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-02-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/16/2/232 |
| _version_ | 1817994328373460992 |
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| author | Beiyuan Fan Xiufeng Li Deyong Chen Hongshang Peng Junbo Wang Jian Chen |
| author_facet | Beiyuan Fan Xiufeng Li Deyong Chen Hongshang Peng Junbo Wang Jian Chen |
| author_sort | Beiyuan Fan |
| collection | DOAJ |
| description | This article reviews recent developments in microfluidic systems enabling high-throughput characterization of single-cell proteins. Four key perspectives of microfluidic platforms are included in this review: (1) microfluidic fluorescent flow cytometry; (2) droplet based microfluidic flow cytometry; (3) large-array micro wells (microengraving); and (4) large-array micro chambers (barcode microchips). We examine the advantages and limitations of each technique and discuss future research opportunities by focusing on three key performance parameters (absolute quantification, sensitivity, and throughput). |
| first_indexed | 2024-04-14T01:51:25Z |
| format | Article |
| id | doaj.art-36ce5e424a30437ab349ed4ec91934a0 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T01:51:25Z |
| publishDate | 2016-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-36ce5e424a30437ab349ed4ec91934a02022-12-22T02:19:20ZengMDPI AGSensors1424-82202016-02-0116223210.3390/s16020232s16020232Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein CharacterizationBeiyuan Fan0Xiufeng Li1Deyong Chen2Hongshang Peng3Junbo Wang4Jian Chen5State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, ChinaState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, ChinaState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, ChinaSchool of Science, Minzu University of China, Beijing 100081, ChinaState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, ChinaState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, ChinaThis article reviews recent developments in microfluidic systems enabling high-throughput characterization of single-cell proteins. Four key perspectives of microfluidic platforms are included in this review: (1) microfluidic fluorescent flow cytometry; (2) droplet based microfluidic flow cytometry; (3) large-array micro wells (microengraving); and (4) large-array micro chambers (barcode microchips). We examine the advantages and limitations of each technique and discuss future research opportunities by focusing on three key performance parameters (absolute quantification, sensitivity, and throughput).http://www.mdpi.com/1424-8220/16/2/232microfluidicssingle-cell analysisprotein characterizationhigh-throughput |
| spellingShingle | Beiyuan Fan Xiufeng Li Deyong Chen Hongshang Peng Junbo Wang Jian Chen Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization Sensors microfluidics single-cell analysis protein characterization high-throughput |
| title | Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization |
| title_full | Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization |
| title_fullStr | Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization |
| title_full_unstemmed | Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization |
| title_short | Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization |
| title_sort | development of microfluidic systems enabling high throughput single cell protein characterization |
| topic | microfluidics single-cell analysis protein characterization high-throughput |
| url | http://www.mdpi.com/1424-8220/16/2/232 |
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