Multiplex loop mediated isothermal amplification (m-LAMP) as a point of care technique for diagnosis of malaria
Diagnosis of malaria is a prominent challenge due to the endemic nature of infection. Malaria poses a great threat to global public health. The disease can be diagnosed by several techniques out of which microscopy is a known gold standard. High sensitivity of molecular techniques is making them mor...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Journal of Vector Borne Diseases |
| Subjects: | |
| Online Access: | http://www.jvbd.org/article.asp?issn=0972-9062;year=2022;volume=59;issue=1;spage=29;epage=36;aulast=Sharma |
| _version_ | 1818196268066799616 |
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| author | Supriya Sharma Jaskirat Singh Aparajita Sen Anupkumar R Anvikar |
| author_facet | Supriya Sharma Jaskirat Singh Aparajita Sen Anupkumar R Anvikar |
| author_sort | Supriya Sharma |
| collection | DOAJ |
| description | Diagnosis of malaria is a prominent challenge due to the endemic nature of infection. Malaria poses a great threat to global public health. The disease can be diagnosed by several techniques out of which microscopy is a known gold standard. High sensitivity of molecular techniques is making them more reliable and popular as tools for diagnosis of malaria. However, new methods are required which can fulfill the criteria of being Point of Care Test (POCT) as defined by WHO. Loop-mediated isothermal amplification (LAMP) technique amplifies DNA in an isothermal condition, and surpasses the disadvantages of conventional molecular techniques such as polymerase chain reaction. Multiplex LAMP, a modification of LAMP may emerge as a new POC for malaria diagnosis. This review deals with the use of LAMP and multiplex LAMP in diagnosis of malaria and its prospective use as point of care techniques. |
| first_indexed | 2024-12-12T01:31:23Z |
| format | Article |
| id | doaj.art-36d04b190e674b60817364bcb3a66e58 |
| institution | Directory Open Access Journal |
| issn | 0972-9062 |
| language | English |
| last_indexed | 2024-12-12T01:31:23Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Vector Borne Diseases |
| spelling | doaj.art-36d04b190e674b60817364bcb3a66e582022-12-22T00:42:58ZengWolters Kluwer Medknow PublicationsJournal of Vector Borne Diseases0972-90622022-01-01591293610.4103/0972-9062.331409Multiplex loop mediated isothermal amplification (m-LAMP) as a point of care technique for diagnosis of malariaSupriya SharmaJaskirat SinghAparajita SenAnupkumar R AnvikarDiagnosis of malaria is a prominent challenge due to the endemic nature of infection. Malaria poses a great threat to global public health. The disease can be diagnosed by several techniques out of which microscopy is a known gold standard. High sensitivity of molecular techniques is making them more reliable and popular as tools for diagnosis of malaria. However, new methods are required which can fulfill the criteria of being Point of Care Test (POCT) as defined by WHO. Loop-mediated isothermal amplification (LAMP) technique amplifies DNA in an isothermal condition, and surpasses the disadvantages of conventional molecular techniques such as polymerase chain reaction. Multiplex LAMP, a modification of LAMP may emerge as a new POC for malaria diagnosis. This review deals with the use of LAMP and multiplex LAMP in diagnosis of malaria and its prospective use as point of care techniques.http://www.jvbd.org/article.asp?issn=0972-9062;year=2022;volume=59;issue=1;spage=29;epage=36;aulast=Sharmamalaria; diagnosis; loop mediated isothermal amplification (lamp); multiplex; point of care (poc) |
| spellingShingle | Supriya Sharma Jaskirat Singh Aparajita Sen Anupkumar R Anvikar Multiplex loop mediated isothermal amplification (m-LAMP) as a point of care technique for diagnosis of malaria Journal of Vector Borne Diseases malaria; diagnosis; loop mediated isothermal amplification (lamp); multiplex; point of care (poc) |
| title | Multiplex loop mediated isothermal amplification (m-LAMP) as a point of care technique for diagnosis of malaria |
| title_full | Multiplex loop mediated isothermal amplification (m-LAMP) as a point of care technique for diagnosis of malaria |
| title_fullStr | Multiplex loop mediated isothermal amplification (m-LAMP) as a point of care technique for diagnosis of malaria |
| title_full_unstemmed | Multiplex loop mediated isothermal amplification (m-LAMP) as a point of care technique for diagnosis of malaria |
| title_short | Multiplex loop mediated isothermal amplification (m-LAMP) as a point of care technique for diagnosis of malaria |
| title_sort | multiplex loop mediated isothermal amplification m lamp as a point of care technique for diagnosis of malaria |
| topic | malaria; diagnosis; loop mediated isothermal amplification (lamp); multiplex; point of care (poc) |
| url | http://www.jvbd.org/article.asp?issn=0972-9062;year=2022;volume=59;issue=1;spage=29;epage=36;aulast=Sharma |
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