Synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3T3-L1 cells
Synthesis and accumulation of the recently identified prostaglandin F2α receptor regulatory protein (FPRP) was found to correlate closely with lipid droplet accumulation by 3T3-L1 preadipose cells. FPRP, a transmembrane glycoprotein, has been shown to regulate the binding of ligand to certain seven-...
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| Format: | Article |
| Language: | English |
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Elsevier
1998-06-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520325396 |
| _version_ | 1818716119022698496 |
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| author | D.J. Orlicky J.G. Lieber C.L. Morin R.M. Evans |
| author_facet | D.J. Orlicky J.G. Lieber C.L. Morin R.M. Evans |
| author_sort | D.J. Orlicky |
| collection | DOAJ |
| description | Synthesis and accumulation of the recently identified prostaglandin F2α receptor regulatory protein (FPRP) was found to correlate closely with lipid droplet accumulation by 3T3-L1 preadipose cells. FPRP, a transmembrane glycoprotein, has been shown to regulate the binding of ligand to certain seven-transmembrane receptors. Anti-FPRP immunohistochemistry, Western blotting, and metabolic labeling/immunoprecipitation experiments demonstrated that FPRP was not detectable in undifferentiated 3T3-L1 cells. Interestingly, low levels of FPRP mRNA were detected in the undifferentiated 3T3-L1 cells. After induction of adipose differentiation, FPRP mRNA increased ∼3 fold whereas FPRP synthesis increased ∼50 fold. Differentiation induction with either dexamethasone/insulin/isobutylmethylxanthine or the thiazolidinedione derivative ADD 4743 were both effective at inducing FPRP accumulation and accumulation of lipid droplets. By co-immunohistochemical and lipid staining, greater than 99% of the cells accumulating lipid droplets possessed FPRP. FPRP mRNA and protein are also found in rat adipose tissue. Treatment of 3T3-L1 cells with an FPRP anti-sense oligonucleotide during differentiation decreased FPRP accumulation and resulted in a decrease in lipid droplets without altering the level of induction of a late marker of adipocyte differentiation, glycerol-3-phosphate dehydrogenase activity. Transient expression of an FPRP cDNA in undifferentiated 3T3-L1 cells was insufficient to induce lipid droplet accumulation.—Orlicky, D. J., J. G. Lieber, C. L. Morin, and R. M. Evans. Synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3T3-L1 cells. J. Lipid Res. 1998. 39: 1152–1161. |
| first_indexed | 2024-12-17T19:14:11Z |
| format | Article |
| id | doaj.art-36d1a51925584d8e974dbdbac4ef1bc1 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T19:14:11Z |
| publishDate | 1998-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-36d1a51925584d8e974dbdbac4ef1bc12022-12-21T21:35:48ZengElsevierJournal of Lipid Research0022-22751998-06-0139611521161Synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3T3-L1 cellsD.J. Orlicky0J.G. Lieber1C.L. Morin2R.M. Evans3To whom correspondence should be addressed.; Department of Pathology, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, CO 80262; Colorado Cancer Center, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, CO 80262Department of Pathology, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, CO 80262Center for Human Nutrition, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, CO 80262Department of Pathology, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, CO 80262Synthesis and accumulation of the recently identified prostaglandin F2α receptor regulatory protein (FPRP) was found to correlate closely with lipid droplet accumulation by 3T3-L1 preadipose cells. FPRP, a transmembrane glycoprotein, has been shown to regulate the binding of ligand to certain seven-transmembrane receptors. Anti-FPRP immunohistochemistry, Western blotting, and metabolic labeling/immunoprecipitation experiments demonstrated that FPRP was not detectable in undifferentiated 3T3-L1 cells. Interestingly, low levels of FPRP mRNA were detected in the undifferentiated 3T3-L1 cells. After induction of adipose differentiation, FPRP mRNA increased ∼3 fold whereas FPRP synthesis increased ∼50 fold. Differentiation induction with either dexamethasone/insulin/isobutylmethylxanthine or the thiazolidinedione derivative ADD 4743 were both effective at inducing FPRP accumulation and accumulation of lipid droplets. By co-immunohistochemical and lipid staining, greater than 99% of the cells accumulating lipid droplets possessed FPRP. FPRP mRNA and protein are also found in rat adipose tissue. Treatment of 3T3-L1 cells with an FPRP anti-sense oligonucleotide during differentiation decreased FPRP accumulation and resulted in a decrease in lipid droplets without altering the level of induction of a late marker of adipocyte differentiation, glycerol-3-phosphate dehydrogenase activity. Transient expression of an FPRP cDNA in undifferentiated 3T3-L1 cells was insufficient to induce lipid droplet accumulation.—Orlicky, D. J., J. G. Lieber, C. L. Morin, and R. M. Evans. Synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3T3-L1 cells. J. Lipid Res. 1998. 39: 1152–1161.http://www.sciencedirect.com/science/article/pii/S00222275203253963T3-L1 adipocytesadipogenesisprostaglandin F2αreceptor regulatory proteinlipid accumulationdexamethasone |
| spellingShingle | D.J. Orlicky J.G. Lieber C.L. Morin R.M. Evans Synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3T3-L1 cells Journal of Lipid Research 3T3-L1 adipocytes adipogenesis prostaglandin F2α receptor regulatory protein lipid accumulation dexamethasone |
| title | Synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3T3-L1 cells |
| title_full | Synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3T3-L1 cells |
| title_fullStr | Synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3T3-L1 cells |
| title_full_unstemmed | Synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3T3-L1 cells |
| title_short | Synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3T3-L1 cells |
| title_sort | synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3t3 l1 cells |
| topic | 3T3-L1 adipocytes adipogenesis prostaglandin F2α receptor regulatory protein lipid accumulation dexamethasone |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520325396 |
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