TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines
Measurement of telomerase activity in clinically obtained tumor samples may provide important information for use as both a diagnostic marker and a prognostic indicator for patient outcome. In order to evaluate telomerase activity in tumor tissue without radiolabeling the product, we developed a sim...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2002-01-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100009 |
| _version_ | 1828326905778012160 |
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| author | C.A. Dalla Torre R.M.B. Maciel N.A. Pinheiro J.A.D. Andrade S.R.C. de Toledo L.L. Villa J.M. Cerutti |
| author_facet | C.A. Dalla Torre R.M.B. Maciel N.A. Pinheiro J.A.D. Andrade S.R.C. de Toledo L.L. Villa J.M. Cerutti |
| author_sort | C.A. Dalla Torre |
| collection | DOAJ |
| description | Measurement of telomerase activity in clinically obtained tumor samples may provide important information for use as both a diagnostic marker and a prognostic indicator for patient outcome. In order to evaluate telomerase activity in tumor tissue without radiolabeling the product, we developed a simple telomeric repeat amplification protocol-silver-staining assay that is less time-consuming, is safe and requires minimal equipment. In addition, we determined the sensitivity of the silver-staining method by using extracts of telomerase-positive thyroid carcinoma cell lines which were serially diluted from 5,000 to 10 cells. Telomerase activity was also assayed in 19 thyroid tumors, 2 normal controls and 27 bone marrow aspirates. The results indicate that the technique permits the detection of telomerase activity from 5000 to as few as 10 cells. We propose that it could be immediately applicable in many laboratories due to the minimal amount of equipment required. |
| first_indexed | 2024-04-13T19:47:01Z |
| format | Article |
| id | doaj.art-36d3a319fd464478a22a20a1faa0a2eb |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-04-13T19:47:01Z |
| publishDate | 2002-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-36d3a319fd464478a22a20a1faa0a2eb2022-12-22T02:32:42ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2002-01-01351656810.1590/S0100-879X2002000100009TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell linesC.A. Dalla TorreR.M.B. MacielN.A. PinheiroJ.A.D. AndradeS.R.C. de ToledoL.L. VillaJ.M. CeruttiMeasurement of telomerase activity in clinically obtained tumor samples may provide important information for use as both a diagnostic marker and a prognostic indicator for patient outcome. In order to evaluate telomerase activity in tumor tissue without radiolabeling the product, we developed a simple telomeric repeat amplification protocol-silver-staining assay that is less time-consuming, is safe and requires minimal equipment. In addition, we determined the sensitivity of the silver-staining method by using extracts of telomerase-positive thyroid carcinoma cell lines which were serially diluted from 5,000 to 10 cells. Telomerase activity was also assayed in 19 thyroid tumors, 2 normal controls and 27 bone marrow aspirates. The results indicate that the technique permits the detection of telomerase activity from 5000 to as few as 10 cells. We propose that it could be immediately applicable in many laboratories due to the minimal amount of equipment required.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100009Telomeric repeat amplification protocolTRAP assayHuman thyroid cancerLeukemiaTelomeraseSilver staining |
| spellingShingle | C.A. Dalla Torre R.M.B. Maciel N.A. Pinheiro J.A.D. Andrade S.R.C. de Toledo L.L. Villa J.M. Cerutti TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines Brazilian Journal of Medical and Biological Research Telomeric repeat amplification protocol TRAP assay Human thyroid cancer Leukemia Telomerase Silver staining |
| title | TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines |
| title_full | TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines |
| title_fullStr | TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines |
| title_full_unstemmed | TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines |
| title_short | TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines |
| title_sort | trap silver staining a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines |
| topic | Telomeric repeat amplification protocol TRAP assay Human thyroid cancer Leukemia Telomerase Silver staining |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100009 |
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