Antiplanktonic and Antibiofilm Activity of <i>Rheum palmatum</i> against <i>Streptococcus oralis</i> and <i>Porphyromonas gingivalis</i>
Periodontitis and peri-implantitis are inflammatory conditions with a high global prevalence. Oral pathogens such as <i>Porphyromonas gingivalis</i> play a crucial role in the development of dysbiotic biofilms associated with both diseases. The aim of our study was to identify plant-deri...
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MDPI AG
2022-05-01
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| author | Nadine Kommerein Nina Vierengel Jonathan Groß Till Opatz Bilal Al-Nawas Lena Katharina Müller-Heupt |
| author_facet | Nadine Kommerein Nina Vierengel Jonathan Groß Till Opatz Bilal Al-Nawas Lena Katharina Müller-Heupt |
| author_sort | Nadine Kommerein |
| collection | DOAJ |
| description | Periodontitis and peri-implantitis are inflammatory conditions with a high global prevalence. Oral pathogens such as <i>Porphyromonas gingivalis</i> play a crucial role in the development of dysbiotic biofilms associated with both diseases. The aim of our study was to identify plant-derived substances which mainly inhibit the growth of “disease promoting bacteria”, by comparing the effect of <i>Rheum palmatum</i> root extract against <i>P. gingivalis</i> and the commensal species <i>Streptococcus oralis</i>. Antiplanktonic activity was determined by measuring optical density and metabolic activity. Antibiofilm activity was quantified using metabolic activity assays and live/dead fluorescence staining combined with confocal laser scanning microscopy. At concentrations of 3.9 mg/L, <i>R. palmatum</i> root extract selectively inhibited planktonic growth of the oral pathogen <i>P. gingivalis</i>, while not inhibiting growth of <i>S. oralis</i>. Selective effects also occurred in mature biofilms, as <i>P. gingivalis</i> was significantly more stressed and inhibited than <i>S. oralis</i>. Our studies show that low concentrations of <i>R. palmatum</i> root extract specifically inhibit <i>P. gingivalis</i> growth, and offer a promising approach for the development of a potential topical agent to prevent alterations in the microbiome due to overgrowth of pathogenic <i>P. gingivalis</i>. |
| first_indexed | 2024-03-10T03:22:55Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T03:22:55Z |
| publishDate | 2022-05-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-36d7b21f259245648cec95cce07c4bcb2023-11-23T12:15:44ZengMDPI AGMicroorganisms2076-26072022-05-0110596510.3390/microorganisms10050965Antiplanktonic and Antibiofilm Activity of <i>Rheum palmatum</i> against <i>Streptococcus oralis</i> and <i>Porphyromonas gingivalis</i>Nadine Kommerein0Nina Vierengel1Jonathan Groß2Till Opatz3Bilal Al-Nawas4Lena Katharina Müller-Heupt5Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, GermanyDepartment of Chemistry, Johannes Gutenberg-University, Duesbergweg 10–14, 55128 Mainz, GermanyDepartment of Chemistry, Johannes Gutenberg-University, Duesbergweg 10–14, 55128 Mainz, GermanyDepartment of Chemistry, Johannes Gutenberg-University, Duesbergweg 10–14, 55128 Mainz, GermanyDepartment of Oral- and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131 Mainz, GermanyDepartment of Oral- and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131 Mainz, GermanyPeriodontitis and peri-implantitis are inflammatory conditions with a high global prevalence. Oral pathogens such as <i>Porphyromonas gingivalis</i> play a crucial role in the development of dysbiotic biofilms associated with both diseases. The aim of our study was to identify plant-derived substances which mainly inhibit the growth of “disease promoting bacteria”, by comparing the effect of <i>Rheum palmatum</i> root extract against <i>P. gingivalis</i> and the commensal species <i>Streptococcus oralis</i>. Antiplanktonic activity was determined by measuring optical density and metabolic activity. Antibiofilm activity was quantified using metabolic activity assays and live/dead fluorescence staining combined with confocal laser scanning microscopy. At concentrations of 3.9 mg/L, <i>R. palmatum</i> root extract selectively inhibited planktonic growth of the oral pathogen <i>P. gingivalis</i>, while not inhibiting growth of <i>S. oralis</i>. Selective effects also occurred in mature biofilms, as <i>P. gingivalis</i> was significantly more stressed and inhibited than <i>S. oralis</i>. Our studies show that low concentrations of <i>R. palmatum</i> root extract specifically inhibit <i>P. gingivalis</i> growth, and offer a promising approach for the development of a potential topical agent to prevent alterations in the microbiome due to overgrowth of pathogenic <i>P. gingivalis</i>.https://www.mdpi.com/2076-2607/10/5/965antibiofilmbiofilmantimicrobialpathogen-selective therapeuticsperiodontitisperi-implantitis |
| spellingShingle | Nadine Kommerein Nina Vierengel Jonathan Groß Till Opatz Bilal Al-Nawas Lena Katharina Müller-Heupt Antiplanktonic and Antibiofilm Activity of <i>Rheum palmatum</i> against <i>Streptococcus oralis</i> and <i>Porphyromonas gingivalis</i> Microorganisms antibiofilm biofilm antimicrobial pathogen-selective therapeutics periodontitis peri-implantitis |
| title | Antiplanktonic and Antibiofilm Activity of <i>Rheum palmatum</i> against <i>Streptococcus oralis</i> and <i>Porphyromonas gingivalis</i> |
| title_full | Antiplanktonic and Antibiofilm Activity of <i>Rheum palmatum</i> against <i>Streptococcus oralis</i> and <i>Porphyromonas gingivalis</i> |
| title_fullStr | Antiplanktonic and Antibiofilm Activity of <i>Rheum palmatum</i> against <i>Streptococcus oralis</i> and <i>Porphyromonas gingivalis</i> |
| title_full_unstemmed | Antiplanktonic and Antibiofilm Activity of <i>Rheum palmatum</i> against <i>Streptococcus oralis</i> and <i>Porphyromonas gingivalis</i> |
| title_short | Antiplanktonic and Antibiofilm Activity of <i>Rheum palmatum</i> against <i>Streptococcus oralis</i> and <i>Porphyromonas gingivalis</i> |
| title_sort | antiplanktonic and antibiofilm activity of i rheum palmatum i against i streptococcus oralis i and i porphyromonas gingivalis i |
| topic | antibiofilm biofilm antimicrobial pathogen-selective therapeutics periodontitis peri-implantitis |
| url | https://www.mdpi.com/2076-2607/10/5/965 |
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