Accelerated wound healing induced by spinach extract in experimental model diabetic rats with streptozotocin
Abstract Patients with diabetes often have difficult-to-heal wounds. Spinacia oleracea extract comprises anti-inflammatory and anti-oxidative compounds; this research, therefore, studied the impact of Spinacia oleracea extracts on ulcer regeneration. This study was conducted on 72 adult Wistar rats...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-42033-0 |
| _version_ | 1827723537237934080 |
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| author | Sara Rahati Mohammad Kamalinezhad Abdolali Ebrahimi Mohammadreza Eshraghian Hamideh Pishva |
| author_facet | Sara Rahati Mohammad Kamalinezhad Abdolali Ebrahimi Mohammadreza Eshraghian Hamideh Pishva |
| author_sort | Sara Rahati |
| collection | DOAJ |
| description | Abstract Patients with diabetes often have difficult-to-heal wounds. Spinacia oleracea extract comprises anti-inflammatory and anti-oxidative compounds; this research, therefore, studied the impact of Spinacia oleracea extracts on ulcer regeneration. This study was conducted on 72 adult Wistar rats (200 $$\pm$$ ± 20 g). They were randomly divided into six groups of twelve. A: Diabetic group receiving normal saline. B: Non-diabetic group receiving normal saline. C: Diabetic group receiving spinach aqueous extract. D: Diabetic group receiving spinach alcoholic extract. E: preventive group that received aqueous extract for 2 months. F: preventive group that received alcoholic extract for 2 months. Ulcer regeneration, vascular endothelium growth factor, blood sugar, and weight changes were measured on days 3, 7, 14, 21, and 30. Macroscopic investigation of the wounds non-diabetic control group, diabetic group, as well as spinach aqueous and alcoholic extract groups, were compared and there were significant changes (P < 0.05). Pathologic examination in the spinach aqueous and alcoholic extract groups, and nondiabetic group than in the diabetic group revealed significant advances (P < 0.05). On the third and seventh days, Vascular endothelium growth factor detected significant differences between groups (P < 0.05). Results indicate that, in regenerating diabetic ulcers, Spinacia oleracea may be effective. It influences the ulcer structure and speed. |
| first_indexed | 2024-03-10T22:02:07Z |
| format | Article |
| id | doaj.art-36d83ddcb7d547208d578aeae2668560 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T22:02:07Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-36d83ddcb7d547208d578aeae26685602023-11-19T12:54:36ZengNature PortfolioScientific Reports2045-23222023-09-0113111210.1038/s41598-023-42033-0Accelerated wound healing induced by spinach extract in experimental model diabetic rats with streptozotocinSara Rahati0Mohammad Kamalinezhad1Abdolali Ebrahimi2Mohammadreza Eshraghian3Hamideh Pishva4Department of Cellular - Molecular Nutrition, School of Nutrition Sciences and Dietetics, Tehran University of Medical SciencesSchool of Pharmacy, Shahid Beheshti University of Medical SciencesDepartment of Pathology, School of Medicine, Shahid Beheshti University of Medical SciencesDepartment of Epidemiology and Biostatistic, School of Public Health, Tehran University of Medical SciencesDepartment of Cellular - Molecular Nutrition, School of Nutrition Sciences and Dietetics, Tehran University of Medical SciencesAbstract Patients with diabetes often have difficult-to-heal wounds. Spinacia oleracea extract comprises anti-inflammatory and anti-oxidative compounds; this research, therefore, studied the impact of Spinacia oleracea extracts on ulcer regeneration. This study was conducted on 72 adult Wistar rats (200 $$\pm$$ ± 20 g). They were randomly divided into six groups of twelve. A: Diabetic group receiving normal saline. B: Non-diabetic group receiving normal saline. C: Diabetic group receiving spinach aqueous extract. D: Diabetic group receiving spinach alcoholic extract. E: preventive group that received aqueous extract for 2 months. F: preventive group that received alcoholic extract for 2 months. Ulcer regeneration, vascular endothelium growth factor, blood sugar, and weight changes were measured on days 3, 7, 14, 21, and 30. Macroscopic investigation of the wounds non-diabetic control group, diabetic group, as well as spinach aqueous and alcoholic extract groups, were compared and there were significant changes (P < 0.05). Pathologic examination in the spinach aqueous and alcoholic extract groups, and nondiabetic group than in the diabetic group revealed significant advances (P < 0.05). On the third and seventh days, Vascular endothelium growth factor detected significant differences between groups (P < 0.05). Results indicate that, in regenerating diabetic ulcers, Spinacia oleracea may be effective. It influences the ulcer structure and speed.https://doi.org/10.1038/s41598-023-42033-0 |
| spellingShingle | Sara Rahati Mohammad Kamalinezhad Abdolali Ebrahimi Mohammadreza Eshraghian Hamideh Pishva Accelerated wound healing induced by spinach extract in experimental model diabetic rats with streptozotocin Scientific Reports |
| title | Accelerated wound healing induced by spinach extract in experimental model diabetic rats with streptozotocin |
| title_full | Accelerated wound healing induced by spinach extract in experimental model diabetic rats with streptozotocin |
| title_fullStr | Accelerated wound healing induced by spinach extract in experimental model diabetic rats with streptozotocin |
| title_full_unstemmed | Accelerated wound healing induced by spinach extract in experimental model diabetic rats with streptozotocin |
| title_short | Accelerated wound healing induced by spinach extract in experimental model diabetic rats with streptozotocin |
| title_sort | accelerated wound healing induced by spinach extract in experimental model diabetic rats with streptozotocin |
| url | https://doi.org/10.1038/s41598-023-42033-0 |
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