Full-length 16S rRNA gene sequencing by PacBio improves taxonomic resolution in human microbiome samples

Full-length 16S rRNA gene sequencing by PacBio improves taxonomic resolution in human microbiome samples

Abstract Background Sequencing variable regions of the 16S rRNA gene (≃300 bp) with Illumina technology is commonly used to study the composition of human microbiota. Unfortunately, short reads are unable to differentiate between highly similar species. Considering that species from the same genus c...

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Bibliographic Details
Main Authors: Elena Buetas, Marta Jordán-López, Andrés López-Roldán, Giuseppe D’Auria, Llucia Martínez-Priego, Griselda De Marco, Miguel Carda-Diéguez, Alex Mira
Format: Article
Language:English
Published: BMC 2024-03-01
Series:BMC Genomics
Subjects:
16S rRNA gene
PacBio
Illumina
Taxonomy
Sequencing
Microbiome
Online Access:https://doi.org/10.1186/s12864-024-10213-5

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