Preparation and evaluation of nattokinase-loaded self-double-emulsifying drug delivery system
In the present study, we prepared nattokinase-loaded self-double-emulsifying drug delivery system (SDEDDS) and investigated its preliminary pharmacodynamics. The type and concentration of oil phase, inner aqueous phase and emulsifier were screened to prepare optimum nattokinase-loaded SDEDDS. Next,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-10-01
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| Series: | Asian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1818087615000409 |
| _version_ | 1818055630942896128 |
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| author | Xiaona Wang Sifan Jiang Xinyue Wang Jie Liao Zongning Yin |
| author_facet | Xiaona Wang Sifan Jiang Xinyue Wang Jie Liao Zongning Yin |
| author_sort | Xiaona Wang |
| collection | DOAJ |
| description | In the present study, we prepared nattokinase-loaded self-double-emulsifying drug delivery system (SDEDDS) and investigated its preliminary pharmacodynamics. The type and concentration of oil phase, inner aqueous phase and emulsifier were screened to prepare optimum nattokinase-loaded SDEDDS. Next, the optimum formulations were characterized based on microstructure, volume-weighted mean droplet size, self-emulsifying rate, yield, storage stability, in vitro release and in vivo pharmacodynamics studies. The water/oil/water multiple emulsions exhibited typical multiple structure, with relatively small volume-weighted mean droplet size 6.0 ± 0.7 μm and high self-emulsifying ability (self-emulsifying time <2 min). Encapsulation of nattokinase was up to 86.8 ± 8.2%. The cumulative release of nattokinase within 8 h was about 30%, exhibiting a sustained release effect. The pharmacodynamics study indicated that nattokinase-loaded SDEDDS could significantly prolong the whole blood clotting time in mouse and effectively improve the carrageenan-induced tail thrombosis compared with nattokinase solution. Moreover, we showed that SDEDDS could successfully self-emulsify into water/oil/water multiple emulsions upon dilution in dispersion medium with gentle stirring and effectively protect nattokinase activity in gastric environment. Our findings suggested that SDEDDS could be a promising strategy for peptide and protein drugs by oral administration. |
| first_indexed | 2024-12-10T12:16:01Z |
| format | Article |
| id | doaj.art-36def9910e5a456a836ab46351cc8d74 |
| institution | Directory Open Access Journal |
| issn | 1818-0876 |
| language | English |
| last_indexed | 2024-12-10T12:16:01Z |
| publishDate | 2015-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Asian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-36def9910e5a456a836ab46351cc8d742022-12-22T01:49:13ZengElsevierAsian Journal of Pharmaceutical Sciences1818-08762015-10-0110538639510.1016/j.ajps.2015.04.005Preparation and evaluation of nattokinase-loaded self-double-emulsifying drug delivery systemXiaona WangSifan JiangXinyue WangJie LiaoZongning YinIn the present study, we prepared nattokinase-loaded self-double-emulsifying drug delivery system (SDEDDS) and investigated its preliminary pharmacodynamics. The type and concentration of oil phase, inner aqueous phase and emulsifier were screened to prepare optimum nattokinase-loaded SDEDDS. Next, the optimum formulations were characterized based on microstructure, volume-weighted mean droplet size, self-emulsifying rate, yield, storage stability, in vitro release and in vivo pharmacodynamics studies. The water/oil/water multiple emulsions exhibited typical multiple structure, with relatively small volume-weighted mean droplet size 6.0 ± 0.7 μm and high self-emulsifying ability (self-emulsifying time <2 min). Encapsulation of nattokinase was up to 86.8 ± 8.2%. The cumulative release of nattokinase within 8 h was about 30%, exhibiting a sustained release effect. The pharmacodynamics study indicated that nattokinase-loaded SDEDDS could significantly prolong the whole blood clotting time in mouse and effectively improve the carrageenan-induced tail thrombosis compared with nattokinase solution. Moreover, we showed that SDEDDS could successfully self-emulsify into water/oil/water multiple emulsions upon dilution in dispersion medium with gentle stirring and effectively protect nattokinase activity in gastric environment. Our findings suggested that SDEDDS could be a promising strategy for peptide and protein drugs by oral administration.http://www.sciencedirect.com/science/article/pii/S1818087615000409Self-double-emulsifying drug delivery systemNattokinaseYieldRelease in vitroPharmacodynamics study |
| spellingShingle | Xiaona Wang Sifan Jiang Xinyue Wang Jie Liao Zongning Yin Preparation and evaluation of nattokinase-loaded self-double-emulsifying drug delivery system Asian Journal of Pharmaceutical Sciences Self-double-emulsifying drug delivery system Nattokinase Yield Release in vitro Pharmacodynamics study |
| title | Preparation and evaluation of nattokinase-loaded self-double-emulsifying drug delivery system |
| title_full | Preparation and evaluation of nattokinase-loaded self-double-emulsifying drug delivery system |
| title_fullStr | Preparation and evaluation of nattokinase-loaded self-double-emulsifying drug delivery system |
| title_full_unstemmed | Preparation and evaluation of nattokinase-loaded self-double-emulsifying drug delivery system |
| title_short | Preparation and evaluation of nattokinase-loaded self-double-emulsifying drug delivery system |
| title_sort | preparation and evaluation of nattokinase loaded self double emulsifying drug delivery system |
| topic | Self-double-emulsifying drug delivery system Nattokinase Yield Release in vitro Pharmacodynamics study |
| url | http://www.sciencedirect.com/science/article/pii/S1818087615000409 |
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