Transcriptome Analysis of the Development of Pedicel Abscission Zone in Tomato
Plant organ abscission is a common phenomenon that occurs at a specific position called the abscission zone (AZ). The differentiation and development of the pedicel AZ play important roles in flower and fruit abscission, which are of great significance for abscission in tomatoes before harvest. Prev...
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MDPI AG
2022-09-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/10/865 |
| _version_ | 1827650010136707072 |
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| author | Xiufen Dong Yue Wang Yanyan Yan Huasen Wang |
| author_facet | Xiufen Dong Yue Wang Yanyan Yan Huasen Wang |
| author_sort | Xiufen Dong |
| collection | DOAJ |
| description | Plant organ abscission is a common phenomenon that occurs at a specific position called the abscission zone (AZ). The differentiation and development of the pedicel AZ play important roles in flower and fruit abscission, which are of great significance for abscission in tomatoes before harvest. Previous studies have reported some genes involved in AZ differentiation; however, the genes regulating pedicel AZ cell development in tomatoes after AZ differentiation remain poorly understood. In this study, transcriptome analyses of tomato pedicel AZ samples were performed at 0, 5, 15, and 30 days post-anthesis (DPA). Pedicel AZ growth was mainly observed before 15 DPA. A principal component analysis and a correlation analysis were carried out in order to compare the repeatability and reliability for different samples. We observed 38 up-regulated and 31 down-regulated genes that were significantly altered during 0 to 5 DPA, 5 to 15 DPA, and 0 to 15 DPA, which may play key roles in AZ cell enlargement. GO and KEGG enrichment analyses of the selected DEGs under all three different comparisons were conducted. Auxin-signaling-related genes were analyzed, as well as AUX/IAA, GH3, and small auxin up-regulated RNA (SAUR) gene expression patterns. The presented results provide information on pedicel AZ development, which might help in regulating flower or fruit pedicel abscission in tomato production facilities. |
| first_indexed | 2024-03-09T20:08:31Z |
| format | Article |
| id | doaj.art-36dfed82cff54eaa8542ed8ffdd78146 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T20:08:31Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
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| series | Horticulturae |
| spelling | doaj.art-36dfed82cff54eaa8542ed8ffdd781462023-11-24T00:22:28ZengMDPI AGHorticulturae2311-75242022-09-0181086510.3390/horticulturae8100865Transcriptome Analysis of the Development of Pedicel Abscission Zone in TomatoXiufen Dong0Yue Wang1Yanyan Yan2Huasen Wang3Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, ChinaKey Laboratory for Quality and Safety Control of Subtropical Fruits and Vegetables, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, Ministry of Agriculture and Rural Affairs, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, ChinaCollaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, ChinaKey Laboratory for Quality and Safety Control of Subtropical Fruits and Vegetables, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, Ministry of Agriculture and Rural Affairs, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, ChinaPlant organ abscission is a common phenomenon that occurs at a specific position called the abscission zone (AZ). The differentiation and development of the pedicel AZ play important roles in flower and fruit abscission, which are of great significance for abscission in tomatoes before harvest. Previous studies have reported some genes involved in AZ differentiation; however, the genes regulating pedicel AZ cell development in tomatoes after AZ differentiation remain poorly understood. In this study, transcriptome analyses of tomato pedicel AZ samples were performed at 0, 5, 15, and 30 days post-anthesis (DPA). Pedicel AZ growth was mainly observed before 15 DPA. A principal component analysis and a correlation analysis were carried out in order to compare the repeatability and reliability for different samples. We observed 38 up-regulated and 31 down-regulated genes that were significantly altered during 0 to 5 DPA, 5 to 15 DPA, and 0 to 15 DPA, which may play key roles in AZ cell enlargement. GO and KEGG enrichment analyses of the selected DEGs under all three different comparisons were conducted. Auxin-signaling-related genes were analyzed, as well as AUX/IAA, GH3, and small auxin up-regulated RNA (SAUR) gene expression patterns. The presented results provide information on pedicel AZ development, which might help in regulating flower or fruit pedicel abscission in tomato production facilities.https://www.mdpi.com/2311-7524/8/10/865tomatopedicel AZ developmenttranscriptome analysisauxin signaling |
| spellingShingle | Xiufen Dong Yue Wang Yanyan Yan Huasen Wang Transcriptome Analysis of the Development of Pedicel Abscission Zone in Tomato Horticulturae tomato pedicel AZ development transcriptome analysis auxin signaling |
| title | Transcriptome Analysis of the Development of Pedicel Abscission Zone in Tomato |
| title_full | Transcriptome Analysis of the Development of Pedicel Abscission Zone in Tomato |
| title_fullStr | Transcriptome Analysis of the Development of Pedicel Abscission Zone in Tomato |
| title_full_unstemmed | Transcriptome Analysis of the Development of Pedicel Abscission Zone in Tomato |
| title_short | Transcriptome Analysis of the Development of Pedicel Abscission Zone in Tomato |
| title_sort | transcriptome analysis of the development of pedicel abscission zone in tomato |
| topic | tomato pedicel AZ development transcriptome analysis auxin signaling |
| url | https://www.mdpi.com/2311-7524/8/10/865 |
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