Isolation, identification, molecular and pathogenicity characteristics of an infectious laryngotracheitis virus from Hubei province, China
ABSTRACT: Multiple outbreaks of avian infectious laryngotracheitis (ILT) in chickens, both domestically and internationally, have been directly correlate to widespread vaccine use in affected countries and regions. Phylogenetic and recombination event analyses have demonstrated that avian infectious...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123007903 |
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| author | Chenyang Yi Guohong Li Yinru Mu Shuyue Cui Danping Zhang Qiaoxia Xu Cheng Liang Man Wang Shiwen Zhou Hongbo Zhou Ming Zhong Anding Zhang |
| author_facet | Chenyang Yi Guohong Li Yinru Mu Shuyue Cui Danping Zhang Qiaoxia Xu Cheng Liang Man Wang Shiwen Zhou Hongbo Zhou Ming Zhong Anding Zhang |
| author_sort | Chenyang Yi |
| collection | DOAJ |
| description | ABSTRACT: Multiple outbreaks of avian infectious laryngotracheitis (ILT) in chickens, both domestically and internationally, have been directly correlate to widespread vaccine use in affected countries and regions. Phylogenetic and recombination event analyses have demonstrated that avian infectious laryngotracheitis virus (ILTV) field strains are progressively evolving toward the chicken embryo-origin (CEO) vaccine strain. Even with standardized biosecurity measures and effective prevention and control strategies implemented on large-scale farms, continuous ILT outbreaks result in significant economic losses to the poultry industry worldwide. These outbreaks undoubtedly hinder efforts to control and eradicate ILTV in the future. In this study, an ILTV isolate was successfully obtained by laboratory PCR detection and virus isolation from chickens that exhibited dyspnea and depression on a broiler farm in Hubei Province, China. The isolated strain exhibited robust propagation on chorioallantoic membranes of embryonated eggs, but failed to establish effective infection in chicken hepatocellular carcinoma (LMH) cells. Phylogenetic analysis revealed a unique T441P point mutation in the gJ protein of the isolate. Animal experiments confirmed the virulence of this strain, as it induced mortality in 6-wk-old chickens. This study expands current understanding of the epidemiology, genetic variations, and pathogenicity of ILTV isolates circulating domestically, contributing to the elucidate of ILTV molecular basis of pathogenicity and development of vaccine. |
| first_indexed | 2024-03-08T03:36:00Z |
| format | Article |
| id | doaj.art-36dff9a0947346a7a5d8b699f24b6b24 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-08T03:36:00Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-36dff9a0947346a7a5d8b699f24b6b242024-02-10T04:43:20ZengElsevierPoultry Science0032-57912024-02-011032103271Isolation, identification, molecular and pathogenicity characteristics of an infectious laryngotracheitis virus from Hubei province, ChinaChenyang Yi0Guohong Li1Yinru Mu2Shuyue Cui3Danping Zhang4Qiaoxia Xu5Cheng Liang6Man Wang7Shiwen Zhou8Hongbo Zhou9Ming Zhong10Anding Zhang11State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, ChinaWuhan Keqian Biology Co., Ltd., Wuhan, Hubei 430070, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, ChinaWuhan Keqian Biology Co., Ltd., Wuhan, Hubei 430070, ChinaWuhan Keqian Biology Co., Ltd., Wuhan, Hubei 430070, ChinaWuhan Keqian Biology Co., Ltd., Wuhan, Hubei 430070, ChinaWuhan Keqian Biology Co., Ltd., Wuhan, Hubei 430070, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, ChinaWuhan Keqian Biology Co., Ltd., Wuhan, Hubei 430070, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture of the People's Republic of China, Wuhan, Hubei 430070, China; Corresponding author:ABSTRACT: Multiple outbreaks of avian infectious laryngotracheitis (ILT) in chickens, both domestically and internationally, have been directly correlate to widespread vaccine use in affected countries and regions. Phylogenetic and recombination event analyses have demonstrated that avian infectious laryngotracheitis virus (ILTV) field strains are progressively evolving toward the chicken embryo-origin (CEO) vaccine strain. Even with standardized biosecurity measures and effective prevention and control strategies implemented on large-scale farms, continuous ILT outbreaks result in significant economic losses to the poultry industry worldwide. These outbreaks undoubtedly hinder efforts to control and eradicate ILTV in the future. In this study, an ILTV isolate was successfully obtained by laboratory PCR detection and virus isolation from chickens that exhibited dyspnea and depression on a broiler farm in Hubei Province, China. The isolated strain exhibited robust propagation on chorioallantoic membranes of embryonated eggs, but failed to establish effective infection in chicken hepatocellular carcinoma (LMH) cells. Phylogenetic analysis revealed a unique T441P point mutation in the gJ protein of the isolate. Animal experiments confirmed the virulence of this strain, as it induced mortality in 6-wk-old chickens. This study expands current understanding of the epidemiology, genetic variations, and pathogenicity of ILTV isolates circulating domestically, contributing to the elucidate of ILTV molecular basis of pathogenicity and development of vaccine.http://www.sciencedirect.com/science/article/pii/S0032579123007903infectious laryngotracheitis virusgJ mutationphylogenetic analysisHubei |
| spellingShingle | Chenyang Yi Guohong Li Yinru Mu Shuyue Cui Danping Zhang Qiaoxia Xu Cheng Liang Man Wang Shiwen Zhou Hongbo Zhou Ming Zhong Anding Zhang Isolation, identification, molecular and pathogenicity characteristics of an infectious laryngotracheitis virus from Hubei province, China Poultry Science infectious laryngotracheitis virus gJ mutation phylogenetic analysis Hubei |
| title | Isolation, identification, molecular and pathogenicity characteristics of an infectious laryngotracheitis virus from Hubei province, China |
| title_full | Isolation, identification, molecular and pathogenicity characteristics of an infectious laryngotracheitis virus from Hubei province, China |
| title_fullStr | Isolation, identification, molecular and pathogenicity characteristics of an infectious laryngotracheitis virus from Hubei province, China |
| title_full_unstemmed | Isolation, identification, molecular and pathogenicity characteristics of an infectious laryngotracheitis virus from Hubei province, China |
| title_short | Isolation, identification, molecular and pathogenicity characteristics of an infectious laryngotracheitis virus from Hubei province, China |
| title_sort | isolation identification molecular and pathogenicity characteristics of an infectious laryngotracheitis virus from hubei province china |
| topic | infectious laryngotracheitis virus gJ mutation phylogenetic analysis Hubei |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123007903 |
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