Associated risk factors and pulsed field gel electrophoresis of nasal isolates of Staphylococcus aureus from medical students in a tertiary hospital in Lagos, Nigeria
Staphylococcus aureus infections are growing problems worldwide with important implications in hospitals. The organism is normally present in the nasal vestibule of about 35% of apparently healthy individuals and its carriage varies between different ethnic and age groups. Staphylococcal nasal carri...
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| Format: | Article |
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Elsevier
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| Series: | Brazilian Journal of Infectious Diseases |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100016&lng=en&tlng=en |
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| author | Solayide A. Adesida Olusegun A. Abioye Babajide S. Bamiro Bartholomew I.C. Brai Stella I. Smith Kehinde O. Amisu Deborah U. Ehichioya Folasade T. Ogunsola Akitoye O. Coker |
| author_facet | Solayide A. Adesida Olusegun A. Abioye Babajide S. Bamiro Bartholomew I.C. Brai Stella I. Smith Kehinde O. Amisu Deborah U. Ehichioya Folasade T. Ogunsola Akitoye O. Coker |
| author_sort | Solayide A. Adesida |
| collection | DOAJ |
| description | Staphylococcus aureus infections are growing problems worldwide with important implications in hospitals. The organism is normally present in the nasal vestibule of about 35% of apparently healthy individuals and its carriage varies between different ethnic and age groups. Staphylococcal nasal carriage among health workers is particularly important to establish new clones and track origin of infections during outbreak situations. To determine the carriage rate and compare the pulsed field gel patterns of the strains, nasal swabs were collected from 185 medical students in a teaching hospital in Lagos, Nigeria. Isolates of S. aureus were tested for heamolysin production, methicillin sensitivity and Pulsed Field Gel Electrophoresis (PFGE) was performed. The results showed S.aureus nasal carrier rate of 14% with significant rate among males compared to females. All the isolates produced heamolysin. Antibiotic susceptibility pattern revealed that majority of the isolates was susceptible. Five strains (19%) harboured resistant determinants to penicillin and tetracycline. None of the strains was resistant to methicillin. 44% of the isolates typed by PFGE had type B, the most predominant pulsotype. PFGE A clone exhibited a single resistance phenotype suggesting a strong clonal relationship that could punctual an outbreak in the hospital. The results speculate that nasal carriage among medical personnel could be a function of various risk factors. Personal hygiene and behaviour may however be the means to reducing colonization and spread of S.aureus in our hospitals. |
| first_indexed | 2024-12-13T14:10:05Z |
| format | Article |
| id | doaj.art-36e2ae7dcb0244ddb5be8ac9f5641053 |
| institution | Directory Open Access Journal |
| issn | 1678-4391 |
| language | English |
| last_indexed | 2024-12-13T14:10:05Z |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-36e2ae7dcb0244ddb5be8ac9f56410532022-12-21T23:42:29ZengElsevierBrazilian Journal of Infectious Diseases1678-4391111636910.1590/S1413-86702007000100016S1413-86702007000100016Associated risk factors and pulsed field gel electrophoresis of nasal isolates of Staphylococcus aureus from medical students in a tertiary hospital in Lagos, NigeriaSolayide A. Adesida0Olusegun A. Abioye1Babajide S. Bamiro2Bartholomew I.C. Brai3Stella I. Smith4Kehinde O. Amisu5Deborah U. Ehichioya6Folasade T. Ogunsola7Akitoye O. Coker8Nigerian Institute of Medical ResearchUniversity of LagosUniversity of LagosNigerian Institute of Medical ResearchNigerian Institute of Medical ResearchUniversity of LagosUniversity of LagosUniversity of LagosUniversity of LagosStaphylococcus aureus infections are growing problems worldwide with important implications in hospitals. The organism is normally present in the nasal vestibule of about 35% of apparently healthy individuals and its carriage varies between different ethnic and age groups. Staphylococcal nasal carriage among health workers is particularly important to establish new clones and track origin of infections during outbreak situations. To determine the carriage rate and compare the pulsed field gel patterns of the strains, nasal swabs were collected from 185 medical students in a teaching hospital in Lagos, Nigeria. Isolates of S. aureus were tested for heamolysin production, methicillin sensitivity and Pulsed Field Gel Electrophoresis (PFGE) was performed. The results showed S.aureus nasal carrier rate of 14% with significant rate among males compared to females. All the isolates produced heamolysin. Antibiotic susceptibility pattern revealed that majority of the isolates was susceptible. Five strains (19%) harboured resistant determinants to penicillin and tetracycline. None of the strains was resistant to methicillin. 44% of the isolates typed by PFGE had type B, the most predominant pulsotype. PFGE A clone exhibited a single resistance phenotype suggesting a strong clonal relationship that could punctual an outbreak in the hospital. The results speculate that nasal carriage among medical personnel could be a function of various risk factors. Personal hygiene and behaviour may however be the means to reducing colonization and spread of S.aureus in our hospitals.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100016&lng=en&tlng=enStaphylococcus aureusPFGEnasal isolatescarriage rate |
| spellingShingle | Solayide A. Adesida Olusegun A. Abioye Babajide S. Bamiro Bartholomew I.C. Brai Stella I. Smith Kehinde O. Amisu Deborah U. Ehichioya Folasade T. Ogunsola Akitoye O. Coker Associated risk factors and pulsed field gel electrophoresis of nasal isolates of Staphylococcus aureus from medical students in a tertiary hospital in Lagos, Nigeria Brazilian Journal of Infectious Diseases Staphylococcus aureus PFGE nasal isolates carriage rate |
| title | Associated risk factors and pulsed field gel electrophoresis of nasal isolates of Staphylococcus aureus from medical students in a tertiary hospital in Lagos, Nigeria |
| title_full | Associated risk factors and pulsed field gel electrophoresis of nasal isolates of Staphylococcus aureus from medical students in a tertiary hospital in Lagos, Nigeria |
| title_fullStr | Associated risk factors and pulsed field gel electrophoresis of nasal isolates of Staphylococcus aureus from medical students in a tertiary hospital in Lagos, Nigeria |
| title_full_unstemmed | Associated risk factors and pulsed field gel electrophoresis of nasal isolates of Staphylococcus aureus from medical students in a tertiary hospital in Lagos, Nigeria |
| title_short | Associated risk factors and pulsed field gel electrophoresis of nasal isolates of Staphylococcus aureus from medical students in a tertiary hospital in Lagos, Nigeria |
| title_sort | associated risk factors and pulsed field gel electrophoresis of nasal isolates of staphylococcus aureus from medical students in a tertiary hospital in lagos nigeria |
| topic | Staphylococcus aureus PFGE nasal isolates carriage rate |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100016&lng=en&tlng=en |
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