Introducing a new histologic scoring system for iron deposition in liver in thalassemia major, compared with atomic absorption spectrometry
Introduction: Iron deposition in liver is a major finding in thalassemic patients. Histologic grading of iron deposition is widely used in many institutions and various grading systems have been introduced. The aim of this study was to introduce a new histologic scoring system (Markaze Tebbi Koodak...
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Mattioli1885
2014-08-01
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| Series: | Mediterranean Journal of Hematology and Infectious Diseases |
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| Online Access: | https://mjhid.org/index.php/mjhid/article/view/1759 |
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| author | Fatemeh Elham Mahjoub H. Tavassoli I. Jahanzad Fatemeh Farahmand F, M. Izadyar M. Najafi Sani M. Lamei Rashti |
| author_facet | Fatemeh Elham Mahjoub H. Tavassoli I. Jahanzad Fatemeh Farahmand F, M. Izadyar M. Najafi Sani M. Lamei Rashti |
| author_sort | Fatemeh Elham Mahjoub |
| collection | DOAJ |
| description | Introduction: Iron deposition in liver is a major finding in thalassemic patients. Histologic grading of iron deposition is widely used in many institutions and various grading systems have been introduced.
The aim of this study was to introduce a new histologic scoring system (Markaze Tebbi Koodakan: MTK 1, 2 and 3) which considers size and density of iron granules as well as zone of iron deposition and verify this system with atomic absorption spectrometry (AAS method).
Materials and methods: Liver biopsies from 63 patients were evaluated, 40 (63.5%) were male and 20 (36.5%) were female. The mean age of the patients was 8.01±3.7 and the age range was from 1.8 to 15 years. Iron scoring was performed according to Sindram & Marx and MTK 1-3 scoring systems.Paraffin blocks were processed for AAS and the results of the iron analysis (HIC: hepatic iron concentration) were reported as µmol/g dry liver weight. The statistical analysis was performed using SPSS, version 16 (SPSS Inc., Chicago, IL, USA).
Results: The mean (SD) of hepatic iron concentration was 230.9 (121.2) µmol/g dry weight.
The relationship between the variables HIC, HII (hepatic iron index) and all histological grading of iron (S&M and MTK1-3) was very strong. Significant differences were identified between the mean HIC in scores 1 & 2 of all histological iron scorings (S&M and MTK 1-3), but no significant differences identified in other adjacent scores in all histological iron scorings (S&M and MTK 1, 2 and 3).
Discussion and conclusion: New scoring system introduced by us in this study was very much the same as simple Sindram and Marx classification. So it is concluded that although size of iron granules is important in lower scores, but in higher scores it is not as much important as the zone in which iron granules are deposited. |
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| issn | 2035-3006 |
| language | English |
| last_indexed | 2024-12-19T16:31:33Z |
| publishDate | 2014-08-01 |
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| series | Mediterranean Journal of Hematology and Infectious Diseases |
| spelling | doaj.art-36e3e8e9605e46fbbf3f0c7d93265ed42022-12-21T20:14:09ZengMattioli1885Mediterranean Journal of Hematology and Infectious Diseases2035-30062014-08-011Introducing a new histologic scoring system for iron deposition in liver in thalassemia major, compared with atomic absorption spectrometryFatemeh Elham Mahjoub0H. Tavassoli1I. Jahanzad2Fatemeh Farahmand F,3M. Izadyar4M. Najafi Sani5M. Lamei Rashti6Maternal Fetal & Neonatal Research CenterResident in Pathology, (Children Hospital Affiliated with Tehran University of Medical Sciences).Associate Professor in Pathology, Cancer Institute,(Affiliated with Tehran University of Medical Sciences).Associate Professor in Pediatric Gastroenterology, Children Hospital Affiliated with Tehran University of Medical SciencesAssistant Professor in Pediatric Hematology Oncology, Children Hospital Affiliated with Tehran University of Medical SciencesAssociate Professor in Pediatric Gastroenterology, Children Hospital Affiliated with Tehran University of Medical SciencesProfessor in Physic, Master of Van De Graaff Laboratory, Iran Atomic Energy OrganizationIntroduction: Iron deposition in liver is a major finding in thalassemic patients. Histologic grading of iron deposition is widely used in many institutions and various grading systems have been introduced. The aim of this study was to introduce a new histologic scoring system (Markaze Tebbi Koodakan: MTK 1, 2 and 3) which considers size and density of iron granules as well as zone of iron deposition and verify this system with atomic absorption spectrometry (AAS method). Materials and methods: Liver biopsies from 63 patients were evaluated, 40 (63.5%) were male and 20 (36.5%) were female. The mean age of the patients was 8.01±3.7 and the age range was from 1.8 to 15 years. Iron scoring was performed according to Sindram & Marx and MTK 1-3 scoring systems.Paraffin blocks were processed for AAS and the results of the iron analysis (HIC: hepatic iron concentration) were reported as µmol/g dry liver weight. The statistical analysis was performed using SPSS, version 16 (SPSS Inc., Chicago, IL, USA). Results: The mean (SD) of hepatic iron concentration was 230.9 (121.2) µmol/g dry weight. The relationship between the variables HIC, HII (hepatic iron index) and all histological grading of iron (S&M and MTK1-3) was very strong. Significant differences were identified between the mean HIC in scores 1 & 2 of all histological iron scorings (S&M and MTK 1-3), but no significant differences identified in other adjacent scores in all histological iron scorings (S&M and MTK 1, 2 and 3). Discussion and conclusion: New scoring system introduced by us in this study was very much the same as simple Sindram and Marx classification. So it is concluded that although size of iron granules is important in lower scores, but in higher scores it is not as much important as the zone in which iron granules are deposited.https://mjhid.org/index.php/mjhid/article/view/1759Thalassemia majorLiver iron depositionAtomic absorption spectroscopyHistologic scorings |
| spellingShingle | Fatemeh Elham Mahjoub H. Tavassoli I. Jahanzad Fatemeh Farahmand F, M. Izadyar M. Najafi Sani M. Lamei Rashti Introducing a new histologic scoring system for iron deposition in liver in thalassemia major, compared with atomic absorption spectrometry Mediterranean Journal of Hematology and Infectious Diseases Thalassemia major Liver iron deposition Atomic absorption spectroscopy Histologic scorings |
| title | Introducing a new histologic scoring system for iron deposition in liver in thalassemia major, compared with atomic absorption spectrometry |
| title_full | Introducing a new histologic scoring system for iron deposition in liver in thalassemia major, compared with atomic absorption spectrometry |
| title_fullStr | Introducing a new histologic scoring system for iron deposition in liver in thalassemia major, compared with atomic absorption spectrometry |
| title_full_unstemmed | Introducing a new histologic scoring system for iron deposition in liver in thalassemia major, compared with atomic absorption spectrometry |
| title_short | Introducing a new histologic scoring system for iron deposition in liver in thalassemia major, compared with atomic absorption spectrometry |
| title_sort | introducing a new histologic scoring system for iron deposition in liver in thalassemia major compared with atomic absorption spectrometry |
| topic | Thalassemia major Liver iron deposition Atomic absorption spectroscopy Histologic scorings |
| url | https://mjhid.org/index.php/mjhid/article/view/1759 |
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