Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA)
The aim of this study was to develop and evaluate a padlock probe based on the Rolling Circle Amplification (RCA), which targeted to 16S-23S rDNA region of S. mutans. The specificity of developed padlock probe was tested for DNA within a panel strains, including S. mutans isolated from the saliva an...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2015-02-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100054&lng=en&tlng=en |
| _version_ | 1819293181204758528 |
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| author | Mônica Moreira Douglas Adamoski Jiufeng Sun Mohammad Javad Najafzadeh Mariana Machado Fidelis do Nascimento Renata Rodrigues Gomes Dicler de Sant'Anna Barbieri Chirlei Glienke Débora do Rocio Klisiowicz Vânia Aparecida Vicente |
| author_facet | Mônica Moreira Douglas Adamoski Jiufeng Sun Mohammad Javad Najafzadeh Mariana Machado Fidelis do Nascimento Renata Rodrigues Gomes Dicler de Sant'Anna Barbieri Chirlei Glienke Débora do Rocio Klisiowicz Vânia Aparecida Vicente |
| author_sort | Mônica Moreira |
| collection | DOAJ |
| description | The aim of this study was to develop and evaluate a padlock probe based on the Rolling Circle Amplification (RCA), which targeted to 16S-23S rDNA region of S. mutans. The specificity of developed padlock probe was tested for DNA within a panel strains, including S. mutans isolated from the saliva and reference strains of the genus Streptococcus, as well as total DNA samples of biofilm and saliva. The results were positive either for DNA samples of S. mutans or DNA samples recovered from the biofilm and saliva revealing the specificity of designed padlock probe. The padlock probe based on the RCA was proved to be an effective, reproducible method for S. mutansdetection and demonstrated the possibility of a rapid detection and accurate identification of S. mutans infection. |
| first_indexed | 2024-12-24T04:06:20Z |
| format | Article |
| id | doaj.art-36e52658e8f0498a84622030e5491957 |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-24T04:06:20Z |
| publishDate | 2015-02-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-36e52658e8f0498a84622030e54919572022-12-21T17:16:10ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242015-02-01581546010.1590/S1516-8913201502762S1516-89132015000100054Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA)Mônica MoreiraDouglas AdamoskiJiufeng SunMohammad Javad NajafzadehMariana Machado Fidelis do NascimentoRenata Rodrigues GomesDicler de Sant'Anna BarbieriChirlei GlienkeDébora do Rocio KlisiowiczVânia Aparecida VicenteThe aim of this study was to develop and evaluate a padlock probe based on the Rolling Circle Amplification (RCA), which targeted to 16S-23S rDNA region of S. mutans. The specificity of developed padlock probe was tested for DNA within a panel strains, including S. mutans isolated from the saliva and reference strains of the genus Streptococcus, as well as total DNA samples of biofilm and saliva. The results were positive either for DNA samples of S. mutans or DNA samples recovered from the biofilm and saliva revealing the specificity of designed padlock probe. The padlock probe based on the RCA was proved to be an effective, reproducible method for S. mutansdetection and demonstrated the possibility of a rapid detection and accurate identification of S. mutans infection.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100054&lng=en&tlng=enPadlock probeRCAStreptococcus mutans |
| spellingShingle | Mônica Moreira Douglas Adamoski Jiufeng Sun Mohammad Javad Najafzadeh Mariana Machado Fidelis do Nascimento Renata Rodrigues Gomes Dicler de Sant'Anna Barbieri Chirlei Glienke Débora do Rocio Klisiowicz Vânia Aparecida Vicente Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA) Brazilian Archives of Biology and Technology Padlock probe RCA Streptococcus mutans |
| title | Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA) |
| title_full | Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA) |
| title_fullStr | Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA) |
| title_full_unstemmed | Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA) |
| title_short | Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA) |
| title_sort | detection of streptococcus mutans using padlock probe based on rolling circle amplification rca |
| topic | Padlock probe RCA Streptococcus mutans |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100054&lng=en&tlng=en |
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