Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics
The metabolomics and proteomics analysis of saliva, an excellent biofluid that is a rich source of biological compounds, allows for the safe and frequent screening of drugs, their metabolites, and molecular biomarkers of various diseases. One of the most frequently used analytical methods in saliva...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/2/155 |
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| author | Paulina Grocholska Marta Kowalska Remigiusz Bąchor |
| author_facet | Paulina Grocholska Marta Kowalska Remigiusz Bąchor |
| author_sort | Paulina Grocholska |
| collection | DOAJ |
| description | The metabolomics and proteomics analysis of saliva, an excellent biofluid that is a rich source of biological compounds, allows for the safe and frequent screening of drugs, their metabolites, and molecular biomarkers of various diseases. One of the most frequently used analytical methods in saliva analysis is liquid chromatography coupled with mass spectrometry (LC-MS) and tandem mass spectrometry. The low ionisation efficiency of some compounds and a complex matrix makes their identification by MS difficult. Furthermore, quantitative analysis by LC-MS frequently cannot be performed without isotopically labelled standards, which usually have to be specially synthesised. This review presented reports on qualitative and quantitative approaches in salivary metabolomics and proteomics. The purpose of this manuscript was to present the challenges, advances, and future prospects of mass spectrometry, both in the analysis of salivary metabolites and proteins. The presented review should appeal to those interested in the recent advances and trends in qualitative and quantitative mass spectrometry in salivary metabolomics and proteomics, which may facilitate a diagnostic accuracy, the evaluation of treatment efficacy, the early diagnosis of disease, and a forensic investigation of some unapproved drugs for any medical or dietary administration. |
| first_indexed | 2024-03-11T08:26:03Z |
| format | Article |
| id | doaj.art-36e5b3d1c90e4c0ea6bd1b44b7be7cff |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T08:26:03Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-36e5b3d1c90e4c0ea6bd1b44b7be7cff2023-11-16T22:03:40ZengMDPI AGMetabolites2218-19892023-01-0113215510.3390/metabo13020155Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and ProteomicsPaulina Grocholska0Marta Kowalska1Remigiusz Bąchor2Faculty of Chemistry, University of Wroclaw, F. Joliot-Curie 14, 50-383 Wroclaw, PolandFaculty of Chemistry, University of Wroclaw, F. Joliot-Curie 14, 50-383 Wroclaw, PolandFaculty of Chemistry, University of Wroclaw, F. Joliot-Curie 14, 50-383 Wroclaw, PolandThe metabolomics and proteomics analysis of saliva, an excellent biofluid that is a rich source of biological compounds, allows for the safe and frequent screening of drugs, their metabolites, and molecular biomarkers of various diseases. One of the most frequently used analytical methods in saliva analysis is liquid chromatography coupled with mass spectrometry (LC-MS) and tandem mass spectrometry. The low ionisation efficiency of some compounds and a complex matrix makes their identification by MS difficult. Furthermore, quantitative analysis by LC-MS frequently cannot be performed without isotopically labelled standards, which usually have to be specially synthesised. This review presented reports on qualitative and quantitative approaches in salivary metabolomics and proteomics. The purpose of this manuscript was to present the challenges, advances, and future prospects of mass spectrometry, both in the analysis of salivary metabolites and proteins. The presented review should appeal to those interested in the recent advances and trends in qualitative and quantitative mass spectrometry in salivary metabolomics and proteomics, which may facilitate a diagnostic accuracy, the evaluation of treatment efficacy, the early diagnosis of disease, and a forensic investigation of some unapproved drugs for any medical or dietary administration.https://www.mdpi.com/2218-1989/13/2/155salivametabolomicsproteomicsLC-MSquantitative analysisisobaric labelling reagents |
| spellingShingle | Paulina Grocholska Marta Kowalska Remigiusz Bąchor Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics Metabolites saliva metabolomics proteomics LC-MS quantitative analysis isobaric labelling reagents |
| title | Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics |
| title_full | Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics |
| title_fullStr | Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics |
| title_full_unstemmed | Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics |
| title_short | Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics |
| title_sort | qualitative and quantitative mass spectrometry in salivary metabolomics and proteomics |
| topic | saliva metabolomics proteomics LC-MS quantitative analysis isobaric labelling reagents |
| url | https://www.mdpi.com/2218-1989/13/2/155 |
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