1H NMR-based Investigation of Metabolic Response to Electro-Acupuncture Stimulation
Abstract Acupuncture is a traditional Chinese medicine therapy that has been found useful for treating various diseases. The treatments involve the insertion of fine needles at acupoints along specific meridians (meridian specificity). This study aims to investigate the metabolic basis of meridian s...
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-07306-5 |
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| author | Caigui Lin Zhiliang Wei Kian-Kai Cheng Jingjing Xu Guiping Shen Chang She Huan Zhong Xiaorong Chang Jiyang Dong |
| author_facet | Caigui Lin Zhiliang Wei Kian-Kai Cheng Jingjing Xu Guiping Shen Chang She Huan Zhong Xiaorong Chang Jiyang Dong |
| author_sort | Caigui Lin |
| collection | DOAJ |
| description | Abstract Acupuncture is a traditional Chinese medicine therapy that has been found useful for treating various diseases. The treatments involve the insertion of fine needles at acupoints along specific meridians (meridian specificity). This study aims to investigate the metabolic basis of meridian specificity using proton nuclear magnetic resonance (1H NMR)-based metabolomics. Electro-acupuncture (EA) stimulations were performed at acupoints of either Stomach Meridian of Foot-Yangming (SMFY) or Gallbladder Meridian of Foot-Shaoyang (GMFS) in healthy male Sprague Dawley (SD) rats. 1H-NMR spectra datasets of serum, urine, cortex, and stomach tissue extracts from the rats were analysed by multivariate statistical analysis to investigate metabolic perturbations due to EA treatments at different meridians. EA treatment on either the SMFY or GMFS acupoints induced significant variations in 31 metabolites, e.g., amino acids, organic acids, choline esters and glucose. Moreover, a few meridian-specific metabolic changes were found for EA stimulations on the SMFY or GMFS acupoints. Our study demonstrated significant metabolic differences in response to EA stimulations on acupoints of SMFY and GMFS meridians. These results validate the hypothesis that meridian specificity in acupuncture is detectable in the metabolome and demonstrate the feasibility and effectiveness of a metabolomics approach in understanding the mechanism of acupuncture. |
| first_indexed | 2024-12-19T04:29:02Z |
| format | Article |
| id | doaj.art-36ec5ab236ed4afdb4749f4df1c87cd4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:29:02Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-36ec5ab236ed4afdb4749f4df1c87cd42022-12-21T20:35:56ZengNature PortfolioScientific Reports2045-23222017-07-017111310.1038/s41598-017-07306-51H NMR-based Investigation of Metabolic Response to Electro-Acupuncture StimulationCaigui Lin0Zhiliang Wei1Kian-Kai Cheng2Jingjing Xu3Guiping Shen4Chang She5Huan Zhong6Xiaorong Chang7Jiyang Dong8Department of Electronic Science, Fujian Provincial Key Laboratory for Plasma and Magnetic Resonance, Xiamen UniversityDepartment of Electronic Science, Fujian Provincial Key Laboratory for Plasma and Magnetic Resonance, Xiamen UniversityDepartment of Bioprocess & Polymer Engineering and Innovation Centre in Agritechnology, Universiti Teknologi MalaysiaDepartment of Electronic Science, Fujian Provincial Key Laboratory for Plasma and Magnetic Resonance, Xiamen UniversityDepartment of Electronic Science, Fujian Provincial Key Laboratory for Plasma and Magnetic Resonance, Xiamen UniversityCollege of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese MedicineCollege of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese MedicineCollege of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese MedicineDepartment of Electronic Science, Fujian Provincial Key Laboratory for Plasma and Magnetic Resonance, Xiamen UniversityAbstract Acupuncture is a traditional Chinese medicine therapy that has been found useful for treating various diseases. The treatments involve the insertion of fine needles at acupoints along specific meridians (meridian specificity). This study aims to investigate the metabolic basis of meridian specificity using proton nuclear magnetic resonance (1H NMR)-based metabolomics. Electro-acupuncture (EA) stimulations were performed at acupoints of either Stomach Meridian of Foot-Yangming (SMFY) or Gallbladder Meridian of Foot-Shaoyang (GMFS) in healthy male Sprague Dawley (SD) rats. 1H-NMR spectra datasets of serum, urine, cortex, and stomach tissue extracts from the rats were analysed by multivariate statistical analysis to investigate metabolic perturbations due to EA treatments at different meridians. EA treatment on either the SMFY or GMFS acupoints induced significant variations in 31 metabolites, e.g., amino acids, organic acids, choline esters and glucose. Moreover, a few meridian-specific metabolic changes were found for EA stimulations on the SMFY or GMFS acupoints. Our study demonstrated significant metabolic differences in response to EA stimulations on acupoints of SMFY and GMFS meridians. These results validate the hypothesis that meridian specificity in acupuncture is detectable in the metabolome and demonstrate the feasibility and effectiveness of a metabolomics approach in understanding the mechanism of acupuncture.https://doi.org/10.1038/s41598-017-07306-5 |
| spellingShingle | Caigui Lin Zhiliang Wei Kian-Kai Cheng Jingjing Xu Guiping Shen Chang She Huan Zhong Xiaorong Chang Jiyang Dong 1H NMR-based Investigation of Metabolic Response to Electro-Acupuncture Stimulation Scientific Reports |
| title | 1H NMR-based Investigation of Metabolic Response to Electro-Acupuncture Stimulation |
| title_full | 1H NMR-based Investigation of Metabolic Response to Electro-Acupuncture Stimulation |
| title_fullStr | 1H NMR-based Investigation of Metabolic Response to Electro-Acupuncture Stimulation |
| title_full_unstemmed | 1H NMR-based Investigation of Metabolic Response to Electro-Acupuncture Stimulation |
| title_short | 1H NMR-based Investigation of Metabolic Response to Electro-Acupuncture Stimulation |
| title_sort | 1h nmr based investigation of metabolic response to electro acupuncture stimulation |
| url | https://doi.org/10.1038/s41598-017-07306-5 |
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