The effect of sex on the quantity and properties of the very low density lipoprotein secreted by the liver in vitro
Livers from normally fed male and female rats were perfused in vitro with different amounts of oleate, and the production and properties of the very low density lipoprotein (VLDL) were studied. The mobility of the VLDL in the zonal ultracentrifuge was dependent on the uptake of free fatty acid and o...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1976-03-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520369984 |
| _version_ | 1818459750870810624 |
|---|---|
| author | C Soler-Argilaga H G Wilcox M Heimberg |
| author_facet | C Soler-Argilaga H G Wilcox M Heimberg |
| author_sort | C Soler-Argilaga |
| collection | DOAJ |
| description | Livers from normally fed male and female rats were perfused in vitro with different amounts of oleate, and the production and properties of the very low density lipoprotein (VLDL) were studied. The mobility of the VLDL in the zonal ultracentrifuge was dependent on the uptake of free fatty acid and on the sex of the animal from which the liver was obtained. A higher proportion of the VLDL secreted by livers from females displayed a more rapid mobility in the zonal ultracentrifuge and, in addition, contained less phospholipid and cholesterol per mole triglyceride than the VLDL from the male, suggestive of larger size of the VLDL secreted by livers from the female rats. Such differences were diminished when the VLDL was compared at equal output of triglyceride but unequal uptake of free fatty acid. These data suggest that the properties of the VLDL are only secondarily modulated by sex, and primarily result from differences in the capacities of livers from either male or female rats to synthesize triglyceride for transport as VLDL. The quantity of triglyceride secreted, regardless of sex, may be an important determinant of both size and number of the VLDL particles. The incorporation of endogenous hepatic fatty acid into VLDL triglyceride was diminished in livers from both sexes by increased uptake of oleate. The greater output of VLDL triglyceride by livers from female animals was dependent on both exogenous and endogenous fatty acids when relatively small quantities of exogenous oleate were available for uptake by the liver. The proportion of palmitate and oleate in the phospholipid of the VLDL secreted by livers from male rats decreased and the content of arachidonate increased with increasing uptake of oleate; no differences were observed in the composition of the phospholipid fatty acids among the various experimental female groups, although these contained more stearate and less oleate and linoleate compared to the male groups. The change of fatty acid composition of the VLDL phospholipid may reflect inclusion of specific types of phospholipid in the VLDL structure for transport of triglyceride from the liver under particular conditions. |
| first_indexed | 2024-12-14T23:19:19Z |
| format | Article |
| id | doaj.art-36ec6bc44c7743baa1f8d88b4e505e20 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T23:19:19Z |
| publishDate | 1976-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-36ec6bc44c7743baa1f8d88b4e505e202022-12-21T22:44:00ZengElsevierJournal of Lipid Research0022-22751976-03-01172139145The effect of sex on the quantity and properties of the very low density lipoprotein secreted by the liver in vitroC Soler-Argilaga0H G Wilcox1M Heimberg2Department of Pharmacology, Vanderbilt University, School of Medicine, Nashville, Tennessee 37232; Department of Pharmacology, University of Missouri, School of Medicine, Columbia, Missouri 65201; Present Address: Department of Pharmacology, University of Missouri, Columbia, Mo. 65201, to which requests for reprints should be sent.Department of Pharmacology, Vanderbilt University, School of Medicine, Nashville, Tennessee 37232; Department of Pharmacology, University of Missouri, School of Medicine, Columbia, Missouri 65201Department of Pharmacology, Vanderbilt University, School of Medicine, Nashville, Tennessee 37232; Department of Pharmacology, University of Missouri, School of Medicine, Columbia, Missouri 65201; Present Address: Department of Pharmacology, University of Missouri, Columbia, Mo. 65201, to which requests for reprints should be sent.Livers from normally fed male and female rats were perfused in vitro with different amounts of oleate, and the production and properties of the very low density lipoprotein (VLDL) were studied. The mobility of the VLDL in the zonal ultracentrifuge was dependent on the uptake of free fatty acid and on the sex of the animal from which the liver was obtained. A higher proportion of the VLDL secreted by livers from females displayed a more rapid mobility in the zonal ultracentrifuge and, in addition, contained less phospholipid and cholesterol per mole triglyceride than the VLDL from the male, suggestive of larger size of the VLDL secreted by livers from the female rats. Such differences were diminished when the VLDL was compared at equal output of triglyceride but unequal uptake of free fatty acid. These data suggest that the properties of the VLDL are only secondarily modulated by sex, and primarily result from differences in the capacities of livers from either male or female rats to synthesize triglyceride for transport as VLDL. The quantity of triglyceride secreted, regardless of sex, may be an important determinant of both size and number of the VLDL particles. The incorporation of endogenous hepatic fatty acid into VLDL triglyceride was diminished in livers from both sexes by increased uptake of oleate. The greater output of VLDL triglyceride by livers from female animals was dependent on both exogenous and endogenous fatty acids when relatively small quantities of exogenous oleate were available for uptake by the liver. The proportion of palmitate and oleate in the phospholipid of the VLDL secreted by livers from male rats decreased and the content of arachidonate increased with increasing uptake of oleate; no differences were observed in the composition of the phospholipid fatty acids among the various experimental female groups, although these contained more stearate and less oleate and linoleate compared to the male groups. The change of fatty acid composition of the VLDL phospholipid may reflect inclusion of specific types of phospholipid in the VLDL structure for transport of triglyceride from the liver under particular conditions.http://www.sciencedirect.com/science/article/pii/S0022227520369984free fatty acidstriglyceridescholesterolphospholipidszonal ultracentrifugationperfused rat liver |
| spellingShingle | C Soler-Argilaga H G Wilcox M Heimberg The effect of sex on the quantity and properties of the very low density lipoprotein secreted by the liver in vitro Journal of Lipid Research free fatty acids triglycerides cholesterol phospholipids zonal ultracentrifugation perfused rat liver |
| title | The effect of sex on the quantity and properties of the very low density lipoprotein secreted by the liver in vitro |
| title_full | The effect of sex on the quantity and properties of the very low density lipoprotein secreted by the liver in vitro |
| title_fullStr | The effect of sex on the quantity and properties of the very low density lipoprotein secreted by the liver in vitro |
| title_full_unstemmed | The effect of sex on the quantity and properties of the very low density lipoprotein secreted by the liver in vitro |
| title_short | The effect of sex on the quantity and properties of the very low density lipoprotein secreted by the liver in vitro |
| title_sort | effect of sex on the quantity and properties of the very low density lipoprotein secreted by the liver in vitro |
| topic | free fatty acids triglycerides cholesterol phospholipids zonal ultracentrifugation perfused rat liver |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520369984 |
| work_keys_str_mv | AT csolerargilaga theeffectofsexonthequantityandpropertiesoftheverylowdensitylipoproteinsecretedbytheliverinvitro AT hgwilcox theeffectofsexonthequantityandpropertiesoftheverylowdensitylipoproteinsecretedbytheliverinvitro AT mheimberg theeffectofsexonthequantityandpropertiesoftheverylowdensitylipoproteinsecretedbytheliverinvitro AT csolerargilaga effectofsexonthequantityandpropertiesoftheverylowdensitylipoproteinsecretedbytheliverinvitro AT hgwilcox effectofsexonthequantityandpropertiesoftheverylowdensitylipoproteinsecretedbytheliverinvitro AT mheimberg effectofsexonthequantityandpropertiesoftheverylowdensitylipoproteinsecretedbytheliverinvitro |