Isolasi Penghambat Aktivitas Enzim Pengubah Angiotensin Dari Protein Susu
ABSTRACT. Three kinds of samles (whey protein containing casenoglycopeptide, whey protein removed casenoglycopeptide and cheese whey powder digested with 7 kinds of proteases at 37 0C for 24 hr (trypsin, protenase-K, actinase-E, thermolysin, and papain) or 25 0C (pepsin and chymotrypsin). Strong inh...
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| Format: | Article |
| Language: | English |
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Animal Husbandry Department, The Faculty of Agriculture, Syiah Kuala University
2016-03-01
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| Series: | Jurnal Agripet |
| Subjects: | |
| Online Access: | https://jurnal.usk.ac.id/agripet/article/view/3212 |
| _version_ | 1797681149951082496 |
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| author | Amhar Abubakar |
| author_facet | Amhar Abubakar |
| author_sort | Amhar Abubakar |
| collection | DOAJ |
| description | ABSTRACT. Three kinds of samles (whey protein containing casenoglycopeptide, whey protein removed casenoglycopeptide and cheese whey powder digested with 7 kinds of proteases at 37 0C for 24 hr (trypsin, protenase-K, actinase-E, thermolysin, and papain) or 25 0C (pepsin and chymotrypsin). Strong inhibotory activity against the angiotensin converting enzyime (ACE, EC 3.4.15.1) was generated in all samples by 5 proteases digestion (pepsin, chymotrypsin, protinase-K, thermolysin and papain). In whey protein removed caseinoglycopeptide digestion by thermolysin induced the highest activity (95,25%). In cheese whey powder, the highest activity was derived by thermolysin (98.25%). On the other hand, week ACE inhibitory activity were derived by trypsin and actinase-E digestion. As no remarkable differences in inhibitory activity were observed between whey protein containing casenoglycopeptide and whey protein removed casenoglycopeptide samples, the bioactive peptides are considered to come mainly not from casenoglycopeptide but from cheese whey powder components. |
| first_indexed | 2024-03-11T23:40:41Z |
| format | Article |
| id | doaj.art-36ecfccfea7546b8a0d47b1d6097a1d4 |
| institution | Directory Open Access Journal |
| issn | 1411-4623 2460-4534 |
| language | English |
| last_indexed | 2024-03-11T23:40:41Z |
| publishDate | 2016-03-01 |
| publisher | Animal Husbandry Department, The Faculty of Agriculture, Syiah Kuala University |
| record_format | Article |
| series | Jurnal Agripet |
| spelling | doaj.art-36ecfccfea7546b8a0d47b1d6097a1d42023-09-19T15:24:04ZengAnimal Husbandry Department, The Faculty of Agriculture, Syiah Kuala UniversityJurnal Agripet1411-46232460-45342016-03-0172333810.17969/agripet.v7i2.32123203Isolasi Penghambat Aktivitas Enzim Pengubah Angiotensin Dari Protein SusuAmhar Abubakar0Jurusan Peternakan, Fakultas Pertanian, Universitas Syiah KualaABSTRACT. Three kinds of samles (whey protein containing casenoglycopeptide, whey protein removed casenoglycopeptide and cheese whey powder digested with 7 kinds of proteases at 37 0C for 24 hr (trypsin, protenase-K, actinase-E, thermolysin, and papain) or 25 0C (pepsin and chymotrypsin). Strong inhibotory activity against the angiotensin converting enzyime (ACE, EC 3.4.15.1) was generated in all samples by 5 proteases digestion (pepsin, chymotrypsin, protinase-K, thermolysin and papain). In whey protein removed caseinoglycopeptide digestion by thermolysin induced the highest activity (95,25%). In cheese whey powder, the highest activity was derived by thermolysin (98.25%). On the other hand, week ACE inhibitory activity were derived by trypsin and actinase-E digestion. As no remarkable differences in inhibitory activity were observed between whey protein containing casenoglycopeptide and whey protein removed casenoglycopeptide samples, the bioactive peptides are considered to come mainly not from casenoglycopeptide but from cheese whey powder components.https://jurnal.usk.ac.id/agripet/article/view/3212isolationmilk proteinangiostensin converting enzymewhey protein |
| spellingShingle | Amhar Abubakar Isolasi Penghambat Aktivitas Enzim Pengubah Angiotensin Dari Protein Susu Jurnal Agripet isolation milk protein angiostensin converting enzyme whey protein |
| title | Isolasi Penghambat Aktivitas Enzim Pengubah Angiotensin Dari Protein Susu |
| title_full | Isolasi Penghambat Aktivitas Enzim Pengubah Angiotensin Dari Protein Susu |
| title_fullStr | Isolasi Penghambat Aktivitas Enzim Pengubah Angiotensin Dari Protein Susu |
| title_full_unstemmed | Isolasi Penghambat Aktivitas Enzim Pengubah Angiotensin Dari Protein Susu |
| title_short | Isolasi Penghambat Aktivitas Enzim Pengubah Angiotensin Dari Protein Susu |
| title_sort | isolasi penghambat aktivitas enzim pengubah angiotensin dari protein susu |
| topic | isolation milk protein angiostensin converting enzyme whey protein |
| url | https://jurnal.usk.ac.id/agripet/article/view/3212 |
| work_keys_str_mv | AT amharabubakar isolasipenghambataktivitasenzimpengubahangiotensindariproteinsusu |