Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle....
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2012-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100013 |
| _version_ | 1818179227510374400 |
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| author | Soumen Naskar Sitangsu M. Deb Saket K. Niranjan Subodh Kumar Deepak Sharma Durgam Sakaram Arjava Sharma |
| author_facet | Soumen Naskar Sitangsu M. Deb Saket K. Niranjan Subodh Kumar Deepak Sharma Durgam Sakaram Arjava Sharma |
| author_sort | Soumen Naskar |
| collection | DOAJ |
| description | In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β1 domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRB*A. |
| first_indexed | 2024-12-11T21:00:31Z |
| format | Article |
| id | doaj.art-36ef8c3859424eb09a3d2171fa8ede01 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-11T21:00:31Z |
| publishDate | 2012-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-36ef8c3859424eb09a3d2171fa8ede012022-12-22T00:50:59ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852012-01-013519598Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)Soumen NaskarSitangsu M. DebSaket K. NiranjanSubodh KumarDeepak SharmaDurgam SakaramArjava SharmaIn the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β1 domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRB*A.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100013water buffalocDNADRBMHCphylogeny |
| spellingShingle | Soumen Naskar Sitangsu M. Deb Saket K. Niranjan Subodh Kumar Deepak Sharma Durgam Sakaram Arjava Sharma Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis) Genetics and Molecular Biology water buffalo cDNA DRB MHC phylogeny |
| title | Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis) |
| title_full | Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis) |
| title_fullStr | Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis) |
| title_full_unstemmed | Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis) |
| title_short | Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis) |
| title_sort | molecular characterization of mhc drb cdna in water buffalo bubalus bubalis |
| topic | water buffalo cDNA DRB MHC phylogeny |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100013 |
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