GC-FID spectroscopic analysis and antioxidant activities of methanolic fraction of Cassia tora leaves
Introduction: From time immemorial, different parts of Cassia tora (Jue ming zi) have found application in Chinese medicine. There is a reputation for the therapeutic properties of the plant's leaves, seeds, and roots. The objective of this study is to ascertain the antioxidant properties and b...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Pharmacological Research - Modern Chinese Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667142523001240 |
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| author | C. Chinelo Nkwocha O. Joshua Felix N. Rosemary Idoko |
| author_facet | C. Chinelo Nkwocha O. Joshua Felix N. Rosemary Idoko |
| author_sort | C. Chinelo Nkwocha |
| collection | DOAJ |
| description | Introduction: From time immemorial, different parts of Cassia tora (Jue ming zi) have found application in Chinese medicine. There is a reputation for the therapeutic properties of the plant's leaves, seeds, and roots. The objective of this study is to ascertain the antioxidant properties and bioactive compounds found in Cassia tora leaf. Materials and method: Phytochemical and antioxidant studies were carried out using standard analytical techniques. Bioactive compounds were analysed using GC FID. Results: Results of the quantitative phytochemical analysis of the methanolic fraction of Cassia tora leaves indicated the presence of saponins (71,760±943.2µg/ml), terpenoids (47,466.70±46.2µg/ml), tannins (21,253.30±46.2µg/ml), flavonoids (14,682.70±40.3µg/ml), phenols (30,986.70±46.2µg/ml), steroids (13,557.30±24.4µg/ml), alkaloids (9770.67±9.2µg/ml), glycosides (5434.67±139.8µg/ml). Using ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) to analyze the antioxidant properties of the methanolic fraction of Cassia tora leaves, it was discovered that the higher the scavenging power of the fraction, the lower the concentration of the standard (ascorbic acid). In comparison to the standard (ascorbic acid), the fraction had the maximum antioxidant capacity at 6.86mg/ml. Total antioxidant power in the fraction ranged from 1.78mg/ml to 16mg/ml. Twenty (20) bioactive chemicals were found in the methanol fraction of Cassia tora leaves, according to the results of the gas chromatography flame ionization detector (GC-FID) investigation. Conclusion: The findings of this current study demonstrate that the leaves of Cassia tora are rich in numerous bioactive compounds and are natural sources of antioxidants. |
| first_indexed | 2024-03-08T22:43:05Z |
| format | Article |
| id | doaj.art-36ef900d92de428a9d2f39253cba07b5 |
| institution | Directory Open Access Journal |
| issn | 2667-1425 |
| language | English |
| last_indexed | 2024-03-08T22:43:05Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Pharmacological Research - Modern Chinese Medicine |
| spelling | doaj.art-36ef900d92de428a9d2f39253cba07b52023-12-17T06:43:03ZengElsevierPharmacological Research - Modern Chinese Medicine2667-14252023-12-019100338GC-FID spectroscopic analysis and antioxidant activities of methanolic fraction of Cassia tora leavesC. Chinelo Nkwocha0O. Joshua Felix1N. Rosemary Idoko2Corresponding author.; Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, NigeriaDepartment of Biochemistry, University of Nigeria, Nsukka, Enugu State, NigeriaDepartment of Biochemistry, University of Nigeria, Nsukka, Enugu State, NigeriaIntroduction: From time immemorial, different parts of Cassia tora (Jue ming zi) have found application in Chinese medicine. There is a reputation for the therapeutic properties of the plant's leaves, seeds, and roots. The objective of this study is to ascertain the antioxidant properties and bioactive compounds found in Cassia tora leaf. Materials and method: Phytochemical and antioxidant studies were carried out using standard analytical techniques. Bioactive compounds were analysed using GC FID. Results: Results of the quantitative phytochemical analysis of the methanolic fraction of Cassia tora leaves indicated the presence of saponins (71,760±943.2µg/ml), terpenoids (47,466.70±46.2µg/ml), tannins (21,253.30±46.2µg/ml), flavonoids (14,682.70±40.3µg/ml), phenols (30,986.70±46.2µg/ml), steroids (13,557.30±24.4µg/ml), alkaloids (9770.67±9.2µg/ml), glycosides (5434.67±139.8µg/ml). Using ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) to analyze the antioxidant properties of the methanolic fraction of Cassia tora leaves, it was discovered that the higher the scavenging power of the fraction, the lower the concentration of the standard (ascorbic acid). In comparison to the standard (ascorbic acid), the fraction had the maximum antioxidant capacity at 6.86mg/ml. Total antioxidant power in the fraction ranged from 1.78mg/ml to 16mg/ml. Twenty (20) bioactive chemicals were found in the methanol fraction of Cassia tora leaves, according to the results of the gas chromatography flame ionization detector (GC-FID) investigation. Conclusion: The findings of this current study demonstrate that the leaves of Cassia tora are rich in numerous bioactive compounds and are natural sources of antioxidants.http://www.sciencedirect.com/science/article/pii/S2667142523001240GC-FIDBioactive compoundsAntioxidantsPhytochemicalsFRAP |
| spellingShingle | C. Chinelo Nkwocha O. Joshua Felix N. Rosemary Idoko GC-FID spectroscopic analysis and antioxidant activities of methanolic fraction of Cassia tora leaves Pharmacological Research - Modern Chinese Medicine GC-FID Bioactive compounds Antioxidants Phytochemicals FRAP |
| title | GC-FID spectroscopic analysis and antioxidant activities of methanolic fraction of Cassia tora leaves |
| title_full | GC-FID spectroscopic analysis and antioxidant activities of methanolic fraction of Cassia tora leaves |
| title_fullStr | GC-FID spectroscopic analysis and antioxidant activities of methanolic fraction of Cassia tora leaves |
| title_full_unstemmed | GC-FID spectroscopic analysis and antioxidant activities of methanolic fraction of Cassia tora leaves |
| title_short | GC-FID spectroscopic analysis and antioxidant activities of methanolic fraction of Cassia tora leaves |
| title_sort | gc fid spectroscopic analysis and antioxidant activities of methanolic fraction of cassia tora leaves |
| topic | GC-FID Bioactive compounds Antioxidants Phytochemicals FRAP |
| url | http://www.sciencedirect.com/science/article/pii/S2667142523001240 |
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