Expanding Avenue of Fast Neutron Mediated Mutagenesis for Crop Improvement
Fast neutron (FN) radiation mediated mutagenesis is a unique approach among the several induced mutagenesis methods being used in plant science in terms of impacted mutations. The FN mutagenesis usually creates deletions from few bases to several million bases (Mb). A library of random deletion gene...
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| Format: | Article |
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MDPI AG
2019-06-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/8/6/164 |
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| author | Surbhi Kumawat Nitika Rana Ruchi Bansal Gautam Vishwakarma Sayaji T. Mehetre Bikram Kishore Das Manish Kumar Satish Kumar Yadav Humira Sonah Tilak Raj Sharma Rupesh Deshmukh |
| author_facet | Surbhi Kumawat Nitika Rana Ruchi Bansal Gautam Vishwakarma Sayaji T. Mehetre Bikram Kishore Das Manish Kumar Satish Kumar Yadav Humira Sonah Tilak Raj Sharma Rupesh Deshmukh |
| author_sort | Surbhi Kumawat |
| collection | DOAJ |
| description | Fast neutron (FN) radiation mediated mutagenesis is a unique approach among the several induced mutagenesis methods being used in plant science in terms of impacted mutations. The FN mutagenesis usually creates deletions from few bases to several million bases (Mb). A library of random deletion generated using FN mutagenesis lines can provide indispensable resources for the reverse genetic approaches. In this review, information from several efforts made using FN mutagenesis has been compiled to understand the type of induced mutations, frequency, and genetic stability. Concerns regarding the utilization of FN mutagenesis technique for a plant with different level of ploidy and genome complexity are discussed. We have highlighted the utility of next-generation sequencing techniques that can be efficiently utilized for the characterization of mutant lines as well as for the mapping of causal mutations. Pros and cons of mapping by mutation (MutMap), mutant chromosome sequencing (MutChromSeq), exon capture, whole genome sequencing, MutRen-Seq, and different tilling approaches that can be used for the detection of FN-induced mutation has also been discussed. Genomic resources developed using the FN mutagenesis have been catalogued wooing to meaningful utilization of the available resources. The information provided here will be helpful for the efficient exploration for the crop improvement programs and for better understanding of genetic regulations. |
| first_indexed | 2024-12-24T01:02:58Z |
| format | Article |
| id | doaj.art-36f68c12a548401f89988dd7bad103c1 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-12-24T01:02:58Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-36f68c12a548401f89988dd7bad103c12022-12-21T17:23:18ZengMDPI AGPlants2223-77472019-06-018616410.3390/plants8060164plants8060164Expanding Avenue of Fast Neutron Mediated Mutagenesis for Crop ImprovementSurbhi Kumawat0Nitika Rana1Ruchi Bansal2Gautam Vishwakarma3Sayaji T. Mehetre4Bikram Kishore Das5Manish Kumar6Satish Kumar Yadav7Humira Sonah8Tilak Raj Sharma9Rupesh Deshmukh10National Agri-food Biotechnology Institute (NABI), Mohali, Punjab 140308, IndiaNational Agri-food Biotechnology Institute (NABI), Mohali, Punjab 140308, IndiaNational Agri-food Biotechnology Institute (NABI), Mohali, Punjab 140308, IndiaNuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, IndiaNuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, IndiaNuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, IndiaDepartment of Seed Science and Technology, College of Horticulture, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh 173230, IndiaNational Bureau of Plant Genetic Resources, New Delhi 110012, IndiaNational Agri-food Biotechnology Institute (NABI), Mohali, Punjab 140308, IndiaNational Agri-food Biotechnology Institute (NABI), Mohali, Punjab 140308, IndiaNational Agri-food Biotechnology Institute (NABI), Mohali, Punjab 140308, IndiaFast neutron (FN) radiation mediated mutagenesis is a unique approach among the several induced mutagenesis methods being used in plant science in terms of impacted mutations. The FN mutagenesis usually creates deletions from few bases to several million bases (Mb). A library of random deletion generated using FN mutagenesis lines can provide indispensable resources for the reverse genetic approaches. In this review, information from several efforts made using FN mutagenesis has been compiled to understand the type of induced mutations, frequency, and genetic stability. Concerns regarding the utilization of FN mutagenesis technique for a plant with different level of ploidy and genome complexity are discussed. We have highlighted the utility of next-generation sequencing techniques that can be efficiently utilized for the characterization of mutant lines as well as for the mapping of causal mutations. Pros and cons of mapping by mutation (MutMap), mutant chromosome sequencing (MutChromSeq), exon capture, whole genome sequencing, MutRen-Seq, and different tilling approaches that can be used for the detection of FN-induced mutation has also been discussed. Genomic resources developed using the FN mutagenesis have been catalogued wooing to meaningful utilization of the available resources. The information provided here will be helpful for the efficient exploration for the crop improvement programs and for better understanding of genetic regulations.https://www.mdpi.com/2223-7747/8/6/164induced mutagenesisfast neutron irradiationmutation mappingdeletagenenext-generation sequencing technologiesreverse genetics |
| spellingShingle | Surbhi Kumawat Nitika Rana Ruchi Bansal Gautam Vishwakarma Sayaji T. Mehetre Bikram Kishore Das Manish Kumar Satish Kumar Yadav Humira Sonah Tilak Raj Sharma Rupesh Deshmukh Expanding Avenue of Fast Neutron Mediated Mutagenesis for Crop Improvement Plants induced mutagenesis fast neutron irradiation mutation mapping deletagene next-generation sequencing technologies reverse genetics |
| title | Expanding Avenue of Fast Neutron Mediated Mutagenesis for Crop Improvement |
| title_full | Expanding Avenue of Fast Neutron Mediated Mutagenesis for Crop Improvement |
| title_fullStr | Expanding Avenue of Fast Neutron Mediated Mutagenesis for Crop Improvement |
| title_full_unstemmed | Expanding Avenue of Fast Neutron Mediated Mutagenesis for Crop Improvement |
| title_short | Expanding Avenue of Fast Neutron Mediated Mutagenesis for Crop Improvement |
| title_sort | expanding avenue of fast neutron mediated mutagenesis for crop improvement |
| topic | induced mutagenesis fast neutron irradiation mutation mapping deletagene next-generation sequencing technologies reverse genetics |
| url | https://www.mdpi.com/2223-7747/8/6/164 |
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