The Different Effect of Decellularized Myocardial Matrix Hydrogel and Decellularized Small Intestinal Submucosa Matrix Hydrogel on Cardiomyocytes and Ischemic Heart
Injectable decellularized matrix hydrogels derived from either myocardium or small intestinal submucosa (pDMYO-gel, pDSIS-gel) have been successfully used for myocardial injury repair. However, the relationship between tissue-specific biological functions and protein composition in these two materia...
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2021-08-01
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| author | Xifeng Yang Shihao Chen Jiaxin Chen Yunqi Liu Ying Bai Shengli Yin Daping Quan |
| author_facet | Xifeng Yang Shihao Chen Jiaxin Chen Yunqi Liu Ying Bai Shengli Yin Daping Quan |
| author_sort | Xifeng Yang |
| collection | DOAJ |
| description | Injectable decellularized matrix hydrogels derived from either myocardium or small intestinal submucosa (pDMYO-gel, pDSIS-gel) have been successfully used for myocardial injury repair. However, the relationship between tissue-specific biological functions and protein composition in these two materials is not clear yet. In this study, the protein composition, mechanical properties, and morphology of these two hydrogels and their effects on the behavior of neonatal rat cardiomyocytes (NRCMs) and human umbilical vein endothelial cells (HUVECs), are investigated. The results show that pDMYO-gel is more conducive to growth, adhesion, spreading, and maintenance of normal NRCM beating, due to its higher proportion of extracellular matrix (ECM) glycoproteins (49.55%) and some unique functional proteins such as annexin-6 (ANXA6), agrin (AGRN), cathepsin D (CTSD) and galectin-1 (LGALS1), whereas pDSIS-gel is more conducive to the proliferation of HUVECs. Animal study shows that pDMYO-gel has a better effect on improving cardiac function, inhibiting myocardial fibrosis and maintaining ventricular wall thickness in acute myocardial infarction models in vivo. Therefore, it is proposed that injectable pDMYO-gel hydrogel may be more suitable for functional recovery of myocardial injuries. |
| first_indexed | 2024-03-10T08:16:57Z |
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| id | doaj.art-36f821c5d23546bf8c382bd95f43042c |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T08:16:57Z |
| publishDate | 2021-08-01 |
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| spelling | doaj.art-36f821c5d23546bf8c382bd95f43042c2023-11-22T10:16:06ZengMDPI AGApplied Sciences2076-34172021-08-011117776810.3390/app11177768The Different Effect of Decellularized Myocardial Matrix Hydrogel and Decellularized Small Intestinal Submucosa Matrix Hydrogel on Cardiomyocytes and Ischemic HeartXifeng Yang0Shihao Chen1Jiaxin Chen2Yunqi Liu3Ying Bai4Shengli Yin5Daping Quan6PCFM Laboratory , Guangdong HPPC Laboratory , School of Chemistry, Sun Yat-sen University, Guangzhou 510275, ChinaPCFM Laboratory , Guangdong HPPC Laboratory , School of Chemistry, Sun Yat-sen University, Guangzhou 510275, ChinaGuangdong Functional Biomaterials Engineering Technology Research Center, School of Materials Science and Engineering, Sun Yat-sen University, Guangzhou 510275, ChinaDepartment of Cardiac Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, ChinaGuangdong Functional Biomaterials Engineering Technology Research Center, School of Materials Science and Engineering, Sun Yat-sen University, Guangzhou 510275, ChinaDepartment of Cardiac Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, ChinaGuangdong Functional Biomaterials Engineering Technology Research Center, School of Materials Science and Engineering, Sun Yat-sen University, Guangzhou 510275, ChinaInjectable decellularized matrix hydrogels derived from either myocardium or small intestinal submucosa (pDMYO-gel, pDSIS-gel) have been successfully used for myocardial injury repair. However, the relationship between tissue-specific biological functions and protein composition in these two materials is not clear yet. In this study, the protein composition, mechanical properties, and morphology of these two hydrogels and their effects on the behavior of neonatal rat cardiomyocytes (NRCMs) and human umbilical vein endothelial cells (HUVECs), are investigated. The results show that pDMYO-gel is more conducive to growth, adhesion, spreading, and maintenance of normal NRCM beating, due to its higher proportion of extracellular matrix (ECM) glycoproteins (49.55%) and some unique functional proteins such as annexin-6 (ANXA6), agrin (AGRN), cathepsin D (CTSD) and galectin-1 (LGALS1), whereas pDSIS-gel is more conducive to the proliferation of HUVECs. Animal study shows that pDMYO-gel has a better effect on improving cardiac function, inhibiting myocardial fibrosis and maintaining ventricular wall thickness in acute myocardial infarction models in vivo. Therefore, it is proposed that injectable pDMYO-gel hydrogel may be more suitable for functional recovery of myocardial injuries.https://www.mdpi.com/2076-3417/11/17/7768decellularized matrix hydrogelsprotein compositioncardiomyocytesproteomic analysismyocardial infarction |
| spellingShingle | Xifeng Yang Shihao Chen Jiaxin Chen Yunqi Liu Ying Bai Shengli Yin Daping Quan The Different Effect of Decellularized Myocardial Matrix Hydrogel and Decellularized Small Intestinal Submucosa Matrix Hydrogel on Cardiomyocytes and Ischemic Heart Applied Sciences decellularized matrix hydrogels protein composition cardiomyocytes proteomic analysis myocardial infarction |
| title | The Different Effect of Decellularized Myocardial Matrix Hydrogel and Decellularized Small Intestinal Submucosa Matrix Hydrogel on Cardiomyocytes and Ischemic Heart |
| title_full | The Different Effect of Decellularized Myocardial Matrix Hydrogel and Decellularized Small Intestinal Submucosa Matrix Hydrogel on Cardiomyocytes and Ischemic Heart |
| title_fullStr | The Different Effect of Decellularized Myocardial Matrix Hydrogel and Decellularized Small Intestinal Submucosa Matrix Hydrogel on Cardiomyocytes and Ischemic Heart |
| title_full_unstemmed | The Different Effect of Decellularized Myocardial Matrix Hydrogel and Decellularized Small Intestinal Submucosa Matrix Hydrogel on Cardiomyocytes and Ischemic Heart |
| title_short | The Different Effect of Decellularized Myocardial Matrix Hydrogel and Decellularized Small Intestinal Submucosa Matrix Hydrogel on Cardiomyocytes and Ischemic Heart |
| title_sort | different effect of decellularized myocardial matrix hydrogel and decellularized small intestinal submucosa matrix hydrogel on cardiomyocytes and ischemic heart |
| topic | decellularized matrix hydrogels protein composition cardiomyocytes proteomic analysis myocardial infarction |
| url | https://www.mdpi.com/2076-3417/11/17/7768 |
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