The Activity of Gold Nanoparticles Synthesized Using Helichrysum odoratissimum Against Cutibacterium acnes Biofilms
The human skin is home to millions of bacteria, fungi, and viruses which form part of a unique microbiome. Commensal microbes, including Cutibacterium acnes can occasionally become opportunistic resulting in the onset of dermatological diseases such as acne. Acne is defined as a chronic inflammatory...
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.675064/full |
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| author | Marco Nuno De Canha Velaphi Clement Thipe Kattesh V. Katti Vusani Mandiwana Michel Lonji Kalombo Suprakas Sinha Ray Rirhandzu Rikhotso Arno Janse van Vuuren Namrita Lall Namrita Lall Namrita Lall |
| author_facet | Marco Nuno De Canha Velaphi Clement Thipe Kattesh V. Katti Vusani Mandiwana Michel Lonji Kalombo Suprakas Sinha Ray Rirhandzu Rikhotso Arno Janse van Vuuren Namrita Lall Namrita Lall Namrita Lall |
| author_sort | Marco Nuno De Canha |
| collection | DOAJ |
| description | The human skin is home to millions of bacteria, fungi, and viruses which form part of a unique microbiome. Commensal microbes, including Cutibacterium acnes can occasionally become opportunistic resulting in the onset of dermatological diseases such as acne. Acne is defined as a chronic inflammatory disorder based on its ability to persist for long periods throughout an individual’s life. The synthesis of gold nanoparticles (AuNPs) was performed using the bottom-up approach by reduction of a gold salt (HAuCl4.3H2O) by the methanol extract (HO-MeOH) and aqueous decoction prepared from the dried aerial parts of Helichrysum odoratissimum (HO-Powder). The HO-MeOH and HO-Powder AuNPs were prepared as unstabilised (−GA) or stabilized (+GA) by the omission or addition of Gum Arabic (GA) as the capping agent. The characterization of the AuNPs was performed using Transmission Electron Microscopy (TEM), dynamic light scattering (DLS), Ultraviolet-Visual spectroscopy (UV-Vis), Thermogravimetric Analysis (TGA), X-Ray Diffraction (XRD) and Zeta-potential. The MBIC50 values for HO-MeOH − GA and HO-MeOH + GA were 1.79 ± 0.78% v/v and 0.22 ± 0.16% v/v, respectively. The HO-Powder AuNPs showed potent inhibition of C. acnes cell adhesion to the 96-well plates. The HO-MeOH − GA and HO-Powder + GA exhibited IC50 of 22.01 ± 6.13% v/v and 11.78 ± 1.78% v/v, respectively. The activity of the AuNPs validated the anti-adhesion activity of the methanol extract in the crude form. The study emphasizes the selectivity of H. odoratissimum AuNPs for the prevention of C. acnes cell adhesion and not antimicrobial activity, which may prevent the emergence of resistant strains of C. acnes through reduced bactericidal or bacteriostatic activity, while targeting mechanisms of pathogenesis. |
| first_indexed | 2024-12-22T10:39:50Z |
| format | Article |
| id | doaj.art-36fac5ba35234b17adf27010b6487682 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-22T10:39:50Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-36fac5ba35234b17adf27010b64876822022-12-21T18:29:03ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-09-01910.3389/fcell.2021.675064675064The Activity of Gold Nanoparticles Synthesized Using Helichrysum odoratissimum Against Cutibacterium acnes BiofilmsMarco Nuno De Canha0Velaphi Clement Thipe1Kattesh V. Katti2Vusani Mandiwana3Michel Lonji Kalombo4Suprakas Sinha Ray5Rirhandzu Rikhotso6Arno Janse van Vuuren7Namrita Lall8Namrita Lall9Namrita Lall10Department of Plant and Soil Sciences, University of Pretoria, Pretoria, South AfricaDepartment of Radiology, Institute of Green Nanotechnology, University of Missouri Columbia, Columbia, MO, United StatesDepartment of Radiology, Institute of Green Nanotechnology, University of Missouri Columbia, Columbia, MO, United StatesChemical Cluster, Centre for Nanostructures and Advanced Materials, Council for Scientific and Industrial Research, Pretoria, South AfricaChemical Cluster, Centre for Nanostructures and Advanced Materials, Council for Scientific and Industrial Research, Pretoria, South AfricaDST/CSIR National Centre for Nanostructured Materials, Council for Scientific and Industrial Research, Pretoria, South AfricaDST/CSIR National Centre for Nanostructured Materials, Council for Scientific and Industrial Research, Pretoria, South AfricaCentre for High Resolution Transmission Electron Microscopy, Nelson Mandela University, Port Elizabeth, South AfricaDepartment of Plant and Soil Sciences, University of Pretoria, Pretoria, South AfricaSchool of Natural Resources, University of Missouri, Columbia, MO, United StatesCollege of Pharmacy, JSS Academy of Higher Education and Research, Mysuru, IndiaThe human skin is home to millions of bacteria, fungi, and viruses which form part of a unique microbiome. Commensal microbes, including Cutibacterium acnes can occasionally become opportunistic resulting in the onset of dermatological diseases such as acne. Acne is defined as a chronic inflammatory disorder based on its ability to persist for long periods throughout an individual’s life. The synthesis of gold nanoparticles (AuNPs) was performed using the bottom-up approach by reduction of a gold salt (HAuCl4.3H2O) by the methanol extract (HO-MeOH) and aqueous decoction prepared from the dried aerial parts of Helichrysum odoratissimum (HO-Powder). The HO-MeOH and HO-Powder AuNPs were prepared as unstabilised (−GA) or stabilized (+GA) by the omission or addition of Gum Arabic (GA) as the capping agent. The characterization of the AuNPs was performed using Transmission Electron Microscopy (TEM), dynamic light scattering (DLS), Ultraviolet-Visual spectroscopy (UV-Vis), Thermogravimetric Analysis (TGA), X-Ray Diffraction (XRD) and Zeta-potential. The MBIC50 values for HO-MeOH − GA and HO-MeOH + GA were 1.79 ± 0.78% v/v and 0.22 ± 0.16% v/v, respectively. The HO-Powder AuNPs showed potent inhibition of C. acnes cell adhesion to the 96-well plates. The HO-MeOH − GA and HO-Powder + GA exhibited IC50 of 22.01 ± 6.13% v/v and 11.78 ± 1.78% v/v, respectively. The activity of the AuNPs validated the anti-adhesion activity of the methanol extract in the crude form. The study emphasizes the selectivity of H. odoratissimum AuNPs for the prevention of C. acnes cell adhesion and not antimicrobial activity, which may prevent the emergence of resistant strains of C. acnes through reduced bactericidal or bacteriostatic activity, while targeting mechanisms of pathogenesis.https://www.frontiersin.org/articles/10.3389/fcell.2021.675064/fullCutibacterium acnesHelichrysum odoratissimumgold nanoparticlesbacterial adhesionbiofilm |
| spellingShingle | Marco Nuno De Canha Velaphi Clement Thipe Kattesh V. Katti Vusani Mandiwana Michel Lonji Kalombo Suprakas Sinha Ray Rirhandzu Rikhotso Arno Janse van Vuuren Namrita Lall Namrita Lall Namrita Lall The Activity of Gold Nanoparticles Synthesized Using Helichrysum odoratissimum Against Cutibacterium acnes Biofilms Frontiers in Cell and Developmental Biology Cutibacterium acnes Helichrysum odoratissimum gold nanoparticles bacterial adhesion biofilm |
| title | The Activity of Gold Nanoparticles Synthesized Using Helichrysum odoratissimum Against Cutibacterium acnes Biofilms |
| title_full | The Activity of Gold Nanoparticles Synthesized Using Helichrysum odoratissimum Against Cutibacterium acnes Biofilms |
| title_fullStr | The Activity of Gold Nanoparticles Synthesized Using Helichrysum odoratissimum Against Cutibacterium acnes Biofilms |
| title_full_unstemmed | The Activity of Gold Nanoparticles Synthesized Using Helichrysum odoratissimum Against Cutibacterium acnes Biofilms |
| title_short | The Activity of Gold Nanoparticles Synthesized Using Helichrysum odoratissimum Against Cutibacterium acnes Biofilms |
| title_sort | activity of gold nanoparticles synthesized using helichrysum odoratissimum against cutibacterium acnes biofilms |
| topic | Cutibacterium acnes Helichrysum odoratissimum gold nanoparticles bacterial adhesion biofilm |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.675064/full |
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