FAM83B is involved in thyroid cancer cell differentiation and migration
Abstract FAM83B has been recently identified as an oncogene, but its role in thyroid cancers (TC) is still unclear. We examined the expression of FAM83B and its possible involvement in cell migration and differentiation, in neoplastic/normal thyroid tissues and in TC human cell lines. FAM83B express...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-12553-2 |
| _version_ | 1818549765618532352 |
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| author | Valentina Cirello Elisa Stellaria Grassi Gabriele Pogliaghi Viola Ghiandai Laura Ermellino Marina Muzza Giacomo Gazzano Luca Persani Carla Colombo Laura Fugazzola |
| author_facet | Valentina Cirello Elisa Stellaria Grassi Gabriele Pogliaghi Viola Ghiandai Laura Ermellino Marina Muzza Giacomo Gazzano Luca Persani Carla Colombo Laura Fugazzola |
| author_sort | Valentina Cirello |
| collection | DOAJ |
| description | Abstract FAM83B has been recently identified as an oncogene, but its role in thyroid cancers (TC) is still unclear. We examined the expression of FAM83B and its possible involvement in cell migration and differentiation, in neoplastic/normal thyroid tissues and in TC human cell lines. FAM83B expression in TC varies according to the tumor histotype, being significantly downregulated in more aggressive and metastatic tissues. FAM83B levels in cell lines recapitulate patients’ samples variations, and its total and cytoplasmic levels decrease upon the induction of migration, together with an increase in its nuclear localization. Similar variations were detected in the primary tumor and in the metastatic tissues from a follicular TC. FAM83B knock down experiments confirmed its role in thyroid differentiation and cell migration, as demonstrated by the reduction of markers of thyroid differentiation and the increase of the mesenchymal marker vimentin. Moreover, the silencing of FAM83B significantly increased cells migration abilities, while not affecting the oncogenic RAS/MAPK/PI3K pathways. Our data indicate for the first time a role for FAM83B in TC cell differentiation and migration. Its expression is reduced in dedifferentiated tumors and its nuclear re-localization could favour distant migration, suggesting that FAM83B should be considered a possible diagnostic and prognostic biomarker. |
| first_indexed | 2024-12-12T08:37:32Z |
| format | Article |
| id | doaj.art-36faeab58c3148129c9845c466e45efb |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T08:37:32Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-36faeab58c3148129c9845c466e45efb2022-12-22T00:30:53ZengNature PortfolioScientific Reports2045-23222022-05-0112111410.1038/s41598-022-12553-2FAM83B is involved in thyroid cancer cell differentiation and migrationValentina Cirello0Elisa Stellaria Grassi1Gabriele Pogliaghi2Viola Ghiandai3Laura Ermellino4Marina Muzza5Giacomo Gazzano6Luca Persani7Carla Colombo8Laura Fugazzola9Laboratory of Endocrine and Metabolic Research, Istituto Auxologico Italiano IRCCSDepartment of Medical Biotechnology and Translational Medicine, University of MilanLaboratory of Endocrine and Metabolic Research, Istituto Auxologico Italiano IRCCSLaboratory of Endocrine and Metabolic Research, Istituto Auxologico Italiano IRCCSPathology Unit, Istituto Auxologico Italiano IRCCSLaboratory of Endocrine and Metabolic Research, Istituto Auxologico Italiano IRCCSPathology Unit, Istituto Auxologico Italiano IRCCSLaboratory of Endocrine and Metabolic Research, Istituto Auxologico Italiano IRCCSDepartment of Pathophysiology and Transplantation, University of MilanDepartment of Pathophysiology and Transplantation, University of MilanAbstract FAM83B has been recently identified as an oncogene, but its role in thyroid cancers (TC) is still unclear. We examined the expression of FAM83B and its possible involvement in cell migration and differentiation, in neoplastic/normal thyroid tissues and in TC human cell lines. FAM83B expression in TC varies according to the tumor histotype, being significantly downregulated in more aggressive and metastatic tissues. FAM83B levels in cell lines recapitulate patients’ samples variations, and its total and cytoplasmic levels decrease upon the induction of migration, together with an increase in its nuclear localization. Similar variations were detected in the primary tumor and in the metastatic tissues from a follicular TC. FAM83B knock down experiments confirmed its role in thyroid differentiation and cell migration, as demonstrated by the reduction of markers of thyroid differentiation and the increase of the mesenchymal marker vimentin. Moreover, the silencing of FAM83B significantly increased cells migration abilities, while not affecting the oncogenic RAS/MAPK/PI3K pathways. Our data indicate for the first time a role for FAM83B in TC cell differentiation and migration. Its expression is reduced in dedifferentiated tumors and its nuclear re-localization could favour distant migration, suggesting that FAM83B should be considered a possible diagnostic and prognostic biomarker.https://doi.org/10.1038/s41598-022-12553-2 |
| spellingShingle | Valentina Cirello Elisa Stellaria Grassi Gabriele Pogliaghi Viola Ghiandai Laura Ermellino Marina Muzza Giacomo Gazzano Luca Persani Carla Colombo Laura Fugazzola FAM83B is involved in thyroid cancer cell differentiation and migration Scientific Reports |
| title | FAM83B is involved in thyroid cancer cell differentiation and migration |
| title_full | FAM83B is involved in thyroid cancer cell differentiation and migration |
| title_fullStr | FAM83B is involved in thyroid cancer cell differentiation and migration |
| title_full_unstemmed | FAM83B is involved in thyroid cancer cell differentiation and migration |
| title_short | FAM83B is involved in thyroid cancer cell differentiation and migration |
| title_sort | fam83b is involved in thyroid cancer cell differentiation and migration |
| url | https://doi.org/10.1038/s41598-022-12553-2 |
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