Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro
Infection by the protozoan parasite Toxoplasma gondii is widely prevalent in humans and animals. To prevent human infection, all meat should be well cooked before consumption, since the parasite is present in skeletal muscle. In this context, the use of skeletal muscle cells (SkMCs) as a cellular mo...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2009-03-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000200007 |
| _version_ | 1797721264746397696 |
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| author | Erick Vaz Guimarães Laís de Carvalho Helene Santos Barbosa |
| author_facet | Erick Vaz Guimarães Laís de Carvalho Helene Santos Barbosa |
| author_sort | Erick Vaz Guimarães |
| collection | DOAJ |
| description | Infection by the protozoan parasite Toxoplasma gondii is widely prevalent in humans and animals. To prevent human infection, all meat should be well cooked before consumption, since the parasite is present in skeletal muscle. In this context, the use of skeletal muscle cells (SkMCs) as a cellular model opens up new approaches to investigate T. gondii-host cell interactions. Immunofluorescent detection of proteins that are stage-specific for bradyzoites indicated that complete cystogenesis of T. gondii in in vitro cultures of SkMCs occurs after 96 h of infection. Ultrastructural analysis showed that, after 48 h of interaction, there were alterations on the parasitophorous vacuole membrane, including greater thickness and increased electron density at the inner face of the membrane. The present study demonstrates the potential use of primary cultures of SkMCs to evaluate different molecular aspects of T. gondii invasion and cystogenesis and presents a promising in vitro model for the screening of drug activities toward tissue cysts and bradyzoites. |
| first_indexed | 2024-03-12T09:31:00Z |
| format | Article |
| id | doaj.art-37003942fe52437a8e963094263eda88 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T09:31:00Z |
| publishDate | 2009-03-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-37003942fe52437a8e963094263eda882023-09-02T13:54:14ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602009-03-01104217017410.1590/S0074-02762009000200007Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitroErick Vaz GuimarãesLaís de CarvalhoHelene Santos BarbosaInfection by the protozoan parasite Toxoplasma gondii is widely prevalent in humans and animals. To prevent human infection, all meat should be well cooked before consumption, since the parasite is present in skeletal muscle. In this context, the use of skeletal muscle cells (SkMCs) as a cellular model opens up new approaches to investigate T. gondii-host cell interactions. Immunofluorescent detection of proteins that are stage-specific for bradyzoites indicated that complete cystogenesis of T. gondii in in vitro cultures of SkMCs occurs after 96 h of infection. Ultrastructural analysis showed that, after 48 h of interaction, there were alterations on the parasitophorous vacuole membrane, including greater thickness and increased electron density at the inner face of the membrane. The present study demonstrates the potential use of primary cultures of SkMCs to evaluate different molecular aspects of T. gondii invasion and cystogenesis and presents a promising in vitro model for the screening of drug activities toward tissue cysts and bradyzoites.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000200007Toxoplasma gonditissue cystsbradyzoitescystogenesisskeletal muscle cells |
| spellingShingle | Erick Vaz Guimarães Laís de Carvalho Helene Santos Barbosa Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro Memorias do Instituto Oswaldo Cruz Toxoplasma gondi tissue cysts bradyzoites cystogenesis skeletal muscle cells |
| title | Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro |
| title_full | Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro |
| title_fullStr | Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro |
| title_full_unstemmed | Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro |
| title_short | Interaction and cystogenesis of Toxoplasma gondii within skeletal muscle cells in vitro |
| title_sort | interaction and cystogenesis of toxoplasma gondii within skeletal muscle cells in vitro |
| topic | Toxoplasma gondi tissue cysts bradyzoites cystogenesis skeletal muscle cells |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000200007 |
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