Acute phase protein response in an experimental model of ovine caseous lymphadenitis
<p>Abstract</p> <p>Background</p> <p>Caseous lymphadenitis (CLA) is a disease of small ruminants caused by <it>Corynebacterium pseudotuberculosis</it>. The pathogenesis of CLA is a slow process, and produces a chronic rather than an acute disease state. Acut...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2007-12-01
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| Series: | BMC Veterinary Research |
| Online Access: | http://www.biomedcentral.com/1746-6148/3/35 |
| _version_ | 1811281099427414016 |
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| author | Lang Tamara L Waterston Mary M Bence Laura Lawson Fraser P Eckersall Peter D Donachie William Fontaine Michael C |
| author_facet | Lang Tamara L Waterston Mary M Bence Laura Lawson Fraser P Eckersall Peter D Donachie William Fontaine Michael C |
| author_sort | Lang Tamara L |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Caseous lymphadenitis (CLA) is a disease of small ruminants caused by <it>Corynebacterium pseudotuberculosis</it>. The pathogenesis of CLA is a slow process, and produces a chronic rather than an acute disease state. Acute phase proteins (APP) such as haptoglobin (Hp) serum amyloid A (SAA) and α<sub>1 </sub>acid glycoprotein (AGP) are produced by the liver and released into the circulation in response to pro-inflammatory cytokines. The concentration of Hp in serum increases in experimental CLA but it is not known if SAA and AGP respond in parallel or have differing response profiles.</p> <p>Results</p> <p>The concentration in serum of Hp, SAA and AGP in 6 sheep challenged with 2 × 10<sup>5 </sup>cells of <it>C. pseudotuberculosis </it>showed significant increases (P < 0.05) compared to 3 unchallenged control sheep. By day 7 post infection. (p.i.) the Hp and SAA concentrations reached mean (± SEM) values of 1.65 ± 0.21 g/L and 18.1 ± 5.2 mg/L respectively. Thereafter, their concentrations fell with no significant difference to those of the control sheep by day 18 p.i.. In contrast, the serum AGP concentration in infected sheep continued to rise to a peak of 0.38 ± 0.05 g/L on day 13 p.i., after which a slow decline occurred, although the mean concentration remained significantly higher (P < 0.05) than the control group up to 29 days p.i.. Specific IgG to phospholidase D of <it>C. pseudotuberculosis </it>became detectable at 11 days p.i. and continued to rise throughout the experiment.</p> <p>Conclusion</p> <p>The serum concentrations of Hp, SAA and AGP were raised in sheep in an experimental model of CLA. An extended response was found for AGP which occurred at a point when the infection was likely to have been transforming from an acute to a chronic phase. The results suggest that AGP could have a role as a marker for chronic conditions in sheep.</p> |
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| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
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| publishDate | 2007-12-01 |
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| spelling | doaj.art-370382a75e81441ba844c6a74a0a4a3c2022-12-22T03:08:36ZengBMCBMC Veterinary Research1746-61482007-12-01313510.1186/1746-6148-3-35Acute phase protein response in an experimental model of ovine caseous lymphadenitisLang Tamara LWaterston Mary MBence LauraLawson Fraser PEckersall Peter DDonachie WilliamFontaine Michael C<p>Abstract</p> <p>Background</p> <p>Caseous lymphadenitis (CLA) is a disease of small ruminants caused by <it>Corynebacterium pseudotuberculosis</it>. The pathogenesis of CLA is a slow process, and produces a chronic rather than an acute disease state. Acute phase proteins (APP) such as haptoglobin (Hp) serum amyloid A (SAA) and α<sub>1 </sub>acid glycoprotein (AGP) are produced by the liver and released into the circulation in response to pro-inflammatory cytokines. The concentration of Hp in serum increases in experimental CLA but it is not known if SAA and AGP respond in parallel or have differing response profiles.</p> <p>Results</p> <p>The concentration in serum of Hp, SAA and AGP in 6 sheep challenged with 2 × 10<sup>5 </sup>cells of <it>C. pseudotuberculosis </it>showed significant increases (P < 0.05) compared to 3 unchallenged control sheep. By day 7 post infection. (p.i.) the Hp and SAA concentrations reached mean (± SEM) values of 1.65 ± 0.21 g/L and 18.1 ± 5.2 mg/L respectively. Thereafter, their concentrations fell with no significant difference to those of the control sheep by day 18 p.i.. In contrast, the serum AGP concentration in infected sheep continued to rise to a peak of 0.38 ± 0.05 g/L on day 13 p.i., after which a slow decline occurred, although the mean concentration remained significantly higher (P < 0.05) than the control group up to 29 days p.i.. Specific IgG to phospholidase D of <it>C. pseudotuberculosis </it>became detectable at 11 days p.i. and continued to rise throughout the experiment.</p> <p>Conclusion</p> <p>The serum concentrations of Hp, SAA and AGP were raised in sheep in an experimental model of CLA. An extended response was found for AGP which occurred at a point when the infection was likely to have been transforming from an acute to a chronic phase. The results suggest that AGP could have a role as a marker for chronic conditions in sheep.</p>http://www.biomedcentral.com/1746-6148/3/35 |
| spellingShingle | Lang Tamara L Waterston Mary M Bence Laura Lawson Fraser P Eckersall Peter D Donachie William Fontaine Michael C Acute phase protein response in an experimental model of ovine caseous lymphadenitis BMC Veterinary Research |
| title | Acute phase protein response in an experimental model of ovine caseous lymphadenitis |
| title_full | Acute phase protein response in an experimental model of ovine caseous lymphadenitis |
| title_fullStr | Acute phase protein response in an experimental model of ovine caseous lymphadenitis |
| title_full_unstemmed | Acute phase protein response in an experimental model of ovine caseous lymphadenitis |
| title_short | Acute phase protein response in an experimental model of ovine caseous lymphadenitis |
| title_sort | acute phase protein response in an experimental model of ovine caseous lymphadenitis |
| url | http://www.biomedcentral.com/1746-6148/3/35 |
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