Anoikis: Roadblock to Cell Transplantation?
Cell therapy, in particular liver cell transplantation, holds great therapeutic potential and is partially hindered by the high rate of apoptosis during cell isolation, cryopreservation, and engraftment. Apoptosis occurring due to cell detachment from the extracellular matrix is a phenomenon termed...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2002-10-01
|
| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/000000002783985404 |
| _version_ | 1828761524627308544 |
|---|---|
| author | Isabel Zvibel Françoise Smets Humberto Soriano |
| author_facet | Isabel Zvibel Françoise Smets Humberto Soriano |
| author_sort | Isabel Zvibel |
| collection | DOAJ |
| description | Cell therapy, in particular liver cell transplantation, holds great therapeutic potential and is partially hindered by the high rate of apoptosis during cell isolation, cryopreservation, and engraftment. Apoptosis occurring due to cell detachment from the extracellular matrix is a phenomenon termed “anoikis. ” The purpose of this review is to describe signaling mechanisms pertinent to anoikis in both immortalized cell lines, but particularly in primary normal epithelial cells. The mechanisms described include integrin signaling and survival molecules, caspase activation, and the role of mitochondrial proteins in anoikis. Strategies to prevent anoikis during isolation and cryopreservation of hepatocytes are discussed. |
| first_indexed | 2024-12-11T01:30:40Z |
| format | Article |
| id | doaj.art-370608f0b5344eaf9f5a783e9247f703 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-11T01:30:40Z |
| publishDate | 2002-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-370608f0b5344eaf9f5a783e9247f7032022-12-22T01:25:22ZengSAGE PublishingCell Transplantation0963-68971555-38922002-10-011110.3727/000000002783985404Anoikis: Roadblock to Cell Transplantation?Isabel Zvibel0Françoise Smets1Humberto Soriano2Gastroenterology Institute, Tel Aviv Sourasky Medical Center, Weizmann 6, Tel Aviv 64239, IsraelDivision of Gastroenterology and Hepatology, Department of Pediatrics, Northwestern University Medical School; Children's Memorial Institute for Education and Research. Siragusa Transplantation Center, Children's Memorial HospitalDivision of Gastroenterology and Hepatology, Department of Pediatrics, Northwestern University Medical School; Children's Memorial Institute for Education and Research. Siragusa Transplantation Center, Children's Memorial HospitalCell therapy, in particular liver cell transplantation, holds great therapeutic potential and is partially hindered by the high rate of apoptosis during cell isolation, cryopreservation, and engraftment. Apoptosis occurring due to cell detachment from the extracellular matrix is a phenomenon termed “anoikis. ” The purpose of this review is to describe signaling mechanisms pertinent to anoikis in both immortalized cell lines, but particularly in primary normal epithelial cells. The mechanisms described include integrin signaling and survival molecules, caspase activation, and the role of mitochondrial proteins in anoikis. Strategies to prevent anoikis during isolation and cryopreservation of hepatocytes are discussed.https://doi.org/10.3727/000000002783985404 |
| spellingShingle | Isabel Zvibel Françoise Smets Humberto Soriano Anoikis: Roadblock to Cell Transplantation? Cell Transplantation |
| title | Anoikis: Roadblock to Cell Transplantation? |
| title_full | Anoikis: Roadblock to Cell Transplantation? |
| title_fullStr | Anoikis: Roadblock to Cell Transplantation? |
| title_full_unstemmed | Anoikis: Roadblock to Cell Transplantation? |
| title_short | Anoikis: Roadblock to Cell Transplantation? |
| title_sort | anoikis roadblock to cell transplantation |
| url | https://doi.org/10.3727/000000002783985404 |
| work_keys_str_mv | AT isabelzvibel anoikisroadblocktocelltransplantation AT francoisesmets anoikisroadblocktocelltransplantation AT humbertosoriano anoikisroadblocktocelltransplantation |