Cytotoxic effects of Annona squamosa leaves against breast cancer cells via apoptotic signaling proteins
Annona squamosa L. is an important medicinal plant used in traditional medicine for the treatment of various diseases. Different parts of A. squamosa L. have various therapeutic effects; however, the anticancer activity of the leaves has not yet been identified. In vitro, MTT, nuclear staining, and...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S101836472200194X |
| _version_ | 1811256141940785152 |
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| author | Rawan Al-Nemari Abir Ben Bacha Abdulrahman Al-Senaidy Mikhlid H. Almutairi Maha Arafah Hadel Al-Saran Nael Abutaha Abdelhabib Semlali |
| author_facet | Rawan Al-Nemari Abir Ben Bacha Abdulrahman Al-Senaidy Mikhlid H. Almutairi Maha Arafah Hadel Al-Saran Nael Abutaha Abdelhabib Semlali |
| author_sort | Rawan Al-Nemari |
| collection | DOAJ |
| description | Annona squamosa L. is an important medicinal plant used in traditional medicine for the treatment of various diseases. Different parts of A. squamosa L. have various therapeutic effects; however, the anticancer activity of the leaves has not yet been identified. In vitro, MTT, nuclear staining, and LDH assays were used to evaluate cell survival and proliferation in cells exposed to the extracts. The effect of the extracts on cell migration was investigated using a monolayer wound repair assay, and the apoptotic effects were evaluated using flow cytometry. A breast cancer model was used to study the effect of the extract on the tumor size, and the expression of different proliferative and apoptotic markers was evaluated by immunohistochemical analysis. At a concentration of 100 µg/mL, A. squamosa leaf extracts exerted strong antiproliferative and cytotoxic effects against various cell lines. The extracts reduced wound closure and strongly induced apoptosis. In vivo study, rats were sacrificed 24 h after the last injection, and tumor size, as well as the expression of proliferative and apoptotic markers, were observed to be greatly affected by treatment with the extracts. Therefore, A. squamosa leaf extract may be developed as a potential novel drug to treat breast cancer in the future. |
| first_indexed | 2024-04-12T17:36:28Z |
| format | Article |
| id | doaj.art-37092fef36e3439ba8c11f8424b1bc3a |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-12T17:36:28Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-37092fef36e3439ba8c11f8424b1bc3a2022-12-22T03:22:58ZengElsevierJournal of King Saud University: Science1018-36472022-06-01344102013Cytotoxic effects of Annona squamosa leaves against breast cancer cells via apoptotic signaling proteinsRawan Al-Nemari0Abir Ben Bacha1Abdulrahman Al-Senaidy2Mikhlid H. Almutairi3Maha Arafah4Hadel Al-Saran5Nael Abutaha6Abdelhabib Semlali7Department of Biochemistry, College of Science, King Saud University, Saudi ArabiaDepartment of Biochemistry, College of Science, King Saud University, Saudi ArabiaDepartment of Biochemistry, College of Science, King Saud University, Saudi ArabiaZoology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaPathology Department, College of Medicine, King Saud University, Riyadh, Saudi ArabiaBiomedical Department, King Fahad Medical City, Riyadh, Saudi ArabiaZoology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaGroupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Québec, Québec, Canada; Corresponding author.Annona squamosa L. is an important medicinal plant used in traditional medicine for the treatment of various diseases. Different parts of A. squamosa L. have various therapeutic effects; however, the anticancer activity of the leaves has not yet been identified. In vitro, MTT, nuclear staining, and LDH assays were used to evaluate cell survival and proliferation in cells exposed to the extracts. The effect of the extracts on cell migration was investigated using a monolayer wound repair assay, and the apoptotic effects were evaluated using flow cytometry. A breast cancer model was used to study the effect of the extract on the tumor size, and the expression of different proliferative and apoptotic markers was evaluated by immunohistochemical analysis. At a concentration of 100 µg/mL, A. squamosa leaf extracts exerted strong antiproliferative and cytotoxic effects against various cell lines. The extracts reduced wound closure and strongly induced apoptosis. In vivo study, rats were sacrificed 24 h after the last injection, and tumor size, as well as the expression of proliferative and apoptotic markers, were observed to be greatly affected by treatment with the extracts. Therefore, A. squamosa leaf extract may be developed as a potential novel drug to treat breast cancer in the future.http://www.sciencedirect.com/science/article/pii/S101836472200194XAnnona squamosaPhytomedicineProliferationApoptosisMigrationEstrogen receptor |
| spellingShingle | Rawan Al-Nemari Abir Ben Bacha Abdulrahman Al-Senaidy Mikhlid H. Almutairi Maha Arafah Hadel Al-Saran Nael Abutaha Abdelhabib Semlali Cytotoxic effects of Annona squamosa leaves against breast cancer cells via apoptotic signaling proteins Journal of King Saud University: Science Annona squamosa Phytomedicine Proliferation Apoptosis Migration Estrogen receptor |
| title | Cytotoxic effects of Annona squamosa leaves against breast cancer cells via apoptotic signaling proteins |
| title_full | Cytotoxic effects of Annona squamosa leaves against breast cancer cells via apoptotic signaling proteins |
| title_fullStr | Cytotoxic effects of Annona squamosa leaves against breast cancer cells via apoptotic signaling proteins |
| title_full_unstemmed | Cytotoxic effects of Annona squamosa leaves against breast cancer cells via apoptotic signaling proteins |
| title_short | Cytotoxic effects of Annona squamosa leaves against breast cancer cells via apoptotic signaling proteins |
| title_sort | cytotoxic effects of annona squamosa leaves against breast cancer cells via apoptotic signaling proteins |
| topic | Annona squamosa Phytomedicine Proliferation Apoptosis Migration Estrogen receptor |
| url | http://www.sciencedirect.com/science/article/pii/S101836472200194X |
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