Preparative fractionation of triglyceride mixtures according to acyl carbon number, using hydroxyalkoxypropyl Sephadex
The present paper describes the use of hydroxyalkoxypropyl Sephadex in a liquid chromatography system. When the column is held at 40°C, and when elution is made with a linear gradient of two solvents, an excellent separation of saturated triglycerides in the region C9–C56 is obtained in 24 hr, even...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1974-01-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520368346 |
| _version_ | 1818743163923202048 |
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| author | Börje Lindqvist Inger Sjögren Rolf Nordin |
| author_facet | Börje Lindqvist Inger Sjögren Rolf Nordin |
| author_sort | Börje Lindqvist |
| collection | DOAJ |
| description | The present paper describes the use of hydroxyalkoxypropyl Sephadex in a liquid chromatography system. When the column is held at 40°C, and when elution is made with a linear gradient of two solvents, an excellent separation of saturated triglycerides in the region C9–C56 is obtained in 24 hr, even with sample loads as high as 0.5 g/cm2 of column.Triglycerides containing unsaturated fatty acids are eluted more rapidly than their saturated homologs, one C-C double bond being equivalent to –1.42 fatty acid carbon atoms. |
| first_indexed | 2024-12-18T02:24:03Z |
| format | Article |
| id | doaj.art-3709a6815a5242cc9e91b4df789dee79 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-18T02:24:03Z |
| publishDate | 1974-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-3709a6815a5242cc9e91b4df789dee792022-12-21T21:24:06ZengElsevierJournal of Lipid Research0022-22751974-01-011516573Preparative fractionation of triglyceride mixtures according to acyl carbon number, using hydroxyalkoxypropyl SephadexBörje Lindqvist0Inger Sjögren1Rolf Nordin2Mjölkcentralen, Fack, S-101 10 Stockholm, SwedenMjölkcentralen, Fack, S-101 10 Stockholm, SwedenMjölkcentralen, Fack, S-101 10 Stockholm, SwedenThe present paper describes the use of hydroxyalkoxypropyl Sephadex in a liquid chromatography system. When the column is held at 40°C, and when elution is made with a linear gradient of two solvents, an excellent separation of saturated triglycerides in the region C9–C56 is obtained in 24 hr, even with sample loads as high as 0.5 g/cm2 of column.Triglycerides containing unsaturated fatty acids are eluted more rapidly than their saturated homologs, one C-C double bond being equivalent to –1.42 fatty acid carbon atoms.http://www.sciencedirect.com/science/article/pii/S0022227520368346gradient elutionunsaturated triglyceridesreversed phasehydrophobic gel |
| spellingShingle | Börje Lindqvist Inger Sjögren Rolf Nordin Preparative fractionation of triglyceride mixtures according to acyl carbon number, using hydroxyalkoxypropyl Sephadex Journal of Lipid Research gradient elution unsaturated triglycerides reversed phase hydrophobic gel |
| title | Preparative fractionation of triglyceride mixtures according to acyl carbon number, using hydroxyalkoxypropyl Sephadex |
| title_full | Preparative fractionation of triglyceride mixtures according to acyl carbon number, using hydroxyalkoxypropyl Sephadex |
| title_fullStr | Preparative fractionation of triglyceride mixtures according to acyl carbon number, using hydroxyalkoxypropyl Sephadex |
| title_full_unstemmed | Preparative fractionation of triglyceride mixtures according to acyl carbon number, using hydroxyalkoxypropyl Sephadex |
| title_short | Preparative fractionation of triglyceride mixtures according to acyl carbon number, using hydroxyalkoxypropyl Sephadex |
| title_sort | preparative fractionation of triglyceride mixtures according to acyl carbon number using hydroxyalkoxypropyl sephadex |
| topic | gradient elution unsaturated triglycerides reversed phase hydrophobic gel |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520368346 |
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