Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16s

Armored scale insects pose a serious threat to habitat conservation across the globe because they include some of the most potent invasive species in the world. They are such a serious concern because their basic morphology, small size, and polyphagous feeding habits often allow them to exist undete...

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Bibliographic Details
Main Authors: Alexander M. Campbell, Andrew J. Lawrence, Caleb B. Hudspath, Matthew E. Gruwell
Format: Article
Language:English
Published: MDPI AG 2014-07-01
Series:Insects
Subjects:
Online Access:http://www.mdpi.com/2075-4450/5/3/528
Description
Summary:Armored scale insects pose a serious threat to habitat conservation across the globe because they include some of the most potent invasive species in the world. They are such a serious concern because their basic morphology, small size, and polyphagous feeding habits often allow them to exist undetected by growers and quarantine experts. In order to provide a potential solution to the problem, we have attempted to elucidate the effectiveness of molecular identification techniques using ribosomal 28s and endosymbiotic 16s rRNA. Sequence data was obtained from many field-collected insects to test the feasibility of identification techniques. A protocol for quick species determination based on sequence data is provided.
ISSN:2075-4450