Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16s
Armored scale insects pose a serious threat to habitat conservation across the globe because they include some of the most potent invasive species in the world. They are such a serious concern because their basic morphology, small size, and polyphagous feeding habits often allow them to exist undete...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-07-01
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| Series: | Insects |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2075-4450/5/3/528 |
| _version_ | 1819042605819756544 |
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| author | Alexander M. Campbell Andrew J. Lawrence Caleb B. Hudspath Matthew E. Gruwell |
| author_facet | Alexander M. Campbell Andrew J. Lawrence Caleb B. Hudspath Matthew E. Gruwell |
| author_sort | Alexander M. Campbell |
| collection | DOAJ |
| description | Armored scale insects pose a serious threat to habitat conservation across the globe because they include some of the most potent invasive species in the world. They are such a serious concern because their basic morphology, small size, and polyphagous feeding habits often allow them to exist undetected by growers and quarantine experts. In order to provide a potential solution to the problem, we have attempted to elucidate the effectiveness of molecular identification techniques using ribosomal 28s and endosymbiotic 16s rRNA. Sequence data was obtained from many field-collected insects to test the feasibility of identification techniques. A protocol for quick species determination based on sequence data is provided. |
| first_indexed | 2024-12-21T09:43:33Z |
| format | Article |
| id | doaj.art-370c3954e6964c7dbfa579a9897f559f |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-12-21T09:43:33Z |
| publishDate | 2014-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-370c3954e6964c7dbfa579a9897f559f2022-12-21T19:08:24ZengMDPI AGInsects2075-44502014-07-015352853810.3390/insects5030528insects5030528Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16sAlexander M. Campbell0Andrew J. Lawrence1Caleb B. Hudspath2Matthew E. Gruwell3Penn State Erie, The Behrend College, 4701 College Dr, Erie, PA 16563, USAPenn State Erie, The Behrend College, 4701 College Dr, Erie, PA 16563, USAPenn State Erie, The Behrend College, 4701 College Dr, Erie, PA 16563, USAPenn State Erie, The Behrend College, 4701 College Dr, Erie, PA 16563, USAArmored scale insects pose a serious threat to habitat conservation across the globe because they include some of the most potent invasive species in the world. They are such a serious concern because their basic morphology, small size, and polyphagous feeding habits often allow them to exist undetected by growers and quarantine experts. In order to provide a potential solution to the problem, we have attempted to elucidate the effectiveness of molecular identification techniques using ribosomal 28s and endosymbiotic 16s rRNA. Sequence data was obtained from many field-collected insects to test the feasibility of identification techniques. A protocol for quick species determination based on sequence data is provided.http://www.mdpi.com/2075-4450/5/3/528Diaspididaearmored scale insectsphylogeneticsmolecular barcodinginvasive speciescryptic species |
| spellingShingle | Alexander M. Campbell Andrew J. Lawrence Caleb B. Hudspath Matthew E. Gruwell Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16s Insects Diaspididae armored scale insects phylogenetics molecular barcoding invasive species cryptic species |
| title | Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16s |
| title_full | Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16s |
| title_fullStr | Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16s |
| title_full_unstemmed | Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16s |
| title_short | Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16s |
| title_sort | molecular identification of diaspididae and elucidation of non native species using the genes 28s and 16s |
| topic | Diaspididae armored scale insects phylogenetics molecular barcoding invasive species cryptic species |
| url | http://www.mdpi.com/2075-4450/5/3/528 |
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