Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning Technique
The application of enzyme-based therapies has received significant attention in modern drug development. Lipases are one of the most versatile enzymes that can be used as therapeutic agents in basic skin care and medical treatment related to excessive sebum production, acne, and inflammation. The tr...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/4/1174 |
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| author | Diána Balogh-Weiser Alexandra Molnár Gergő D. Tóth Gábor Koplányi József Szemes Balázs Decsi Gábor Katona Maryana Salamah Ferenc Ender Anita Kovács Szilvia Berkó Mária Budai-Szűcs György T. Balogh |
| author_facet | Diána Balogh-Weiser Alexandra Molnár Gergő D. Tóth Gábor Koplányi József Szemes Balázs Decsi Gábor Katona Maryana Salamah Ferenc Ender Anita Kovács Szilvia Berkó Mária Budai-Szűcs György T. Balogh |
| author_sort | Diána Balogh-Weiser |
| collection | DOAJ |
| description | The application of enzyme-based therapies has received significant attention in modern drug development. Lipases are one of the most versatile enzymes that can be used as therapeutic agents in basic skin care and medical treatment related to excessive sebum production, acne, and inflammation. The traditional formulations available for skin treatment, such as creams, ointments or gels, are widely applied; however, their use is not always accompanied by good drug penetration properties, stability, or patient adherence. Nanoformulated drugs offer the possibility of combining enzymatic and small molecule formulations, making them a new and exciting alternative in this field. In this study polymeric nanofibrous matrices made of polyvinylpyrrolidone and polylactic acid were developed, entrapping lipases from <i>Candida rugosa</i> and <i>Rizomucor miehei</i> and antibiotic compound nadifloxacin. The effect of the type of polymers and lipases were investigated, and the nanofiber formation process was optimized to provide a promising alternative in topical treatment. Our experiments have shown that entrapment by electrospinning induced two orders of magnitude increase in the specific enzyme activity of lipases. Permeability investigations indicated that all lipase-loaded nanofibrous masks were capable of delivering nadifloxacin to the human epidermis, confirming the viability of electrospinning as a formulation method for topical skin medications. |
| first_indexed | 2024-03-11T04:38:05Z |
| format | Article |
| id | doaj.art-370da88930404a20b82123c6c15dac79 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T04:38:05Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-370da88930404a20b82123c6c15dac792023-11-17T20:53:46ZengMDPI AGPharmaceutics1999-49232023-04-01154117410.3390/pharmaceutics15041174Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning TechniqueDiána Balogh-Weiser0Alexandra Molnár1Gergő D. Tóth2Gábor Koplányi3József Szemes4Balázs Decsi5Gábor Katona6Maryana Salamah7Ferenc Ender8Anita Kovács9Szilvia Berkó10Mária Budai-Szűcs11György T. Balogh12Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, Műegyetem rkp. 3, H-1111 Budapest, HungaryDepartment of Organic Chemistry and Technology, Budapest University of Technology and Economics, Műegyetem rkp. 3, H-1111 Budapest, HungaryDepartment of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, Műegyetem rkp. 3, H-1111 Budapest, HungaryDepartment of Organic Chemistry and Technology, Budapest University of Technology and Economics, Műegyetem rkp. 3, H-1111 Budapest, HungaryDepartment of Organic Chemistry and Technology, Budapest University of Technology and Economics, Műegyetem rkp. 3, H-1111 Budapest, HungaryDepartment of Organic Chemistry and Technology, Budapest University of Technology and Economics, Műegyetem rkp. 3, H-1111 Budapest, HungaryInstitute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, Eötvös u. 6, H-6720 Szeged, HungaryInstitute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, Eötvös u. 6, H-6720 Szeged, HungaryDepartment of Electron Devices, Budapest University of Technology and Economics, Műegyetem rkp. 3, H-1111 Budapest, HungaryInstitute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, Eötvös u. 6, H-6720 Szeged, HungaryInstitute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, Eötvös u. 6, H-6720 Szeged, HungaryInstitute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, Eötvös u. 6, H-6720 Szeged, HungaryIstitute of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, H-6720 Szeged, HungaryThe application of enzyme-based therapies has received significant attention in modern drug development. Lipases are one of the most versatile enzymes that can be used as therapeutic agents in basic skin care and medical treatment related to excessive sebum production, acne, and inflammation. The traditional formulations available for skin treatment, such as creams, ointments or gels, are widely applied; however, their use is not always accompanied by good drug penetration properties, stability, or patient adherence. Nanoformulated drugs offer the possibility of combining enzymatic and small molecule formulations, making them a new and exciting alternative in this field. In this study polymeric nanofibrous matrices made of polyvinylpyrrolidone and polylactic acid were developed, entrapping lipases from <i>Candida rugosa</i> and <i>Rizomucor miehei</i> and antibiotic compound nadifloxacin. The effect of the type of polymers and lipases were investigated, and the nanofiber formation process was optimized to provide a promising alternative in topical treatment. Our experiments have shown that entrapment by electrospinning induced two orders of magnitude increase in the specific enzyme activity of lipases. Permeability investigations indicated that all lipase-loaded nanofibrous masks were capable of delivering nadifloxacin to the human epidermis, confirming the viability of electrospinning as a formulation method for topical skin medications.https://www.mdpi.com/1999-4923/15/4/1174nanoformulationnano maskelectrospinningskin treatmentacne vulgarislipase |
| spellingShingle | Diána Balogh-Weiser Alexandra Molnár Gergő D. Tóth Gábor Koplányi József Szemes Balázs Decsi Gábor Katona Maryana Salamah Ferenc Ender Anita Kovács Szilvia Berkó Mária Budai-Szűcs György T. Balogh Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning Technique Pharmaceutics nanoformulation nano mask electrospinning skin treatment acne vulgaris lipase |
| title | Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning Technique |
| title_full | Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning Technique |
| title_fullStr | Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning Technique |
| title_full_unstemmed | Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning Technique |
| title_short | Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning Technique |
| title_sort | combined nanofibrous face mask co formulation of lipases and antibiotic agent by electrospinning technique |
| topic | nanoformulation nano mask electrospinning skin treatment acne vulgaris lipase |
| url | https://www.mdpi.com/1999-4923/15/4/1174 |
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