Mechanisms of Rostellularia procumbens (L.) Nees on treating chronic glomerulonephritis explored by network pharmacology, RNA-seq, and in vitro experiments
Abstract Background The purpose of this study was to demonstrate the in vitro anti-nephritis activity of Rostellularia procumbens (L.) Nees (R. procumbens) extract and to make a preliminary investigation of its anti-nephritis mechanism. Methods A prediction network was built that describes the relat...
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | BMC Complementary Medicine and Therapies |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12906-023-04079-5 |
| _version_ | 1797769626073956352 |
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| author | Mengfan Wang Yi Zhou Qiuyuan Jian Zhongzhu Ai Shanshan Zhou |
| author_facet | Mengfan Wang Yi Zhou Qiuyuan Jian Zhongzhu Ai Shanshan Zhou |
| author_sort | Mengfan Wang |
| collection | DOAJ |
| description | Abstract Background The purpose of this study was to demonstrate the in vitro anti-nephritis activity of Rostellularia procumbens (L.) Nees (R. procumbens) extract and to make a preliminary investigation of its anti-nephritis mechanism. Methods A prediction network was built that describes the relationship between R. procumbens and CGN. Then, the potential targets for R. procumbens against CGN were imported into the DAVID database for Gene Ontology (GO) biological annotation analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. A lipopolysaccharide (LPS)-stimulated rat mesangial cell HBZY-1 model in vitro was used to examine the anti-inflammatory activity of R. procumbens extract. RNA-seq was utilized to investigate differentially expressed genes (DEGs) and enriched signaling pathways between groups. Finally, qPCR was used for the validation analysis of the experimental results. Results The results of network pharmacology showed that R. procumbens exerts its therapeutic effect on CGN through the AGE-RAGE signaling pathway in diabetic complications, PI3K-Akt, IL-17 signaling pathway, and so on. R. procumbens n-butanol extract (J-NE) can effectively relieve inflammation in HBZY-1. The results of KEGG pathway enrichment suggest that J-NE attenuated CGN was associated with the IL-17 signaling pathway, and the results of RNA-seq were consistent with network pharmacology. Targets enriched in the IL-17 signaling pathway, including Chemokine (C-C motif) ligand 7 (CCL7), Lipocalin 2 (LCN2), Chemokine (C-C motif) ligand 2 (CCL2), and Chemokine (C-X-C motif) ligand 1 (CXCL1), have been identified as crucial targets attenuating CGN by J-NE. Conclusion R. procumbens is a promising pharmacological candidate for the treatment of CGN in the present era. |
| first_indexed | 2024-03-12T21:11:43Z |
| format | Article |
| id | doaj.art-370fd3b4d4aa443d95b902672b9035e3 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-03-12T21:11:43Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-370fd3b4d4aa443d95b902672b9035e32023-07-30T11:07:22ZengBMCBMC Complementary Medicine and Therapies2662-76712023-07-0123111310.1186/s12906-023-04079-5Mechanisms of Rostellularia procumbens (L.) Nees on treating chronic glomerulonephritis explored by network pharmacology, RNA-seq, and in vitro experimentsMengfan Wang0Yi Zhou1Qiuyuan Jian2Zhongzhu Ai3Shanshan Zhou4School of Pharmacy, Hubei University of Chinese MedicineSchool of Pharmacy, Hubei University of Chinese MedicineSchool of Pharmacy, Hubei University of Chinese MedicineSchool of Pharmacy, Hubei University of Chinese MedicineThe First Clinical Medical School, Hubei University of Chinese MedicineAbstract Background The purpose of this study was to demonstrate the in vitro anti-nephritis activity of Rostellularia procumbens (L.) Nees (R. procumbens) extract and to make a preliminary investigation of its anti-nephritis mechanism. Methods A prediction network was built that describes the relationship between R. procumbens and CGN. Then, the potential targets for R. procumbens against CGN were imported into the DAVID database for Gene Ontology (GO) biological annotation analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. A lipopolysaccharide (LPS)-stimulated rat mesangial cell HBZY-1 model in vitro was used to examine the anti-inflammatory activity of R. procumbens extract. RNA-seq was utilized to investigate differentially expressed genes (DEGs) and enriched signaling pathways between groups. Finally, qPCR was used for the validation analysis of the experimental results. Results The results of network pharmacology showed that R. procumbens exerts its therapeutic effect on CGN through the AGE-RAGE signaling pathway in diabetic complications, PI3K-Akt, IL-17 signaling pathway, and so on. R. procumbens n-butanol extract (J-NE) can effectively relieve inflammation in HBZY-1. The results of KEGG pathway enrichment suggest that J-NE attenuated CGN was associated with the IL-17 signaling pathway, and the results of RNA-seq were consistent with network pharmacology. Targets enriched in the IL-17 signaling pathway, including Chemokine (C-C motif) ligand 7 (CCL7), Lipocalin 2 (LCN2), Chemokine (C-C motif) ligand 2 (CCL2), and Chemokine (C-X-C motif) ligand 1 (CXCL1), have been identified as crucial targets attenuating CGN by J-NE. Conclusion R. procumbens is a promising pharmacological candidate for the treatment of CGN in the present era.https://doi.org/10.1186/s12906-023-04079-5Rostellularia procumbens (L.) NeesChronic glomerulonephritisNetwork pharmacologyRNA-seqMechanism |
| spellingShingle | Mengfan Wang Yi Zhou Qiuyuan Jian Zhongzhu Ai Shanshan Zhou Mechanisms of Rostellularia procumbens (L.) Nees on treating chronic glomerulonephritis explored by network pharmacology, RNA-seq, and in vitro experiments BMC Complementary Medicine and Therapies Rostellularia procumbens (L.) Nees Chronic glomerulonephritis Network pharmacology RNA-seq Mechanism |
| title | Mechanisms of Rostellularia procumbens (L.) Nees on treating chronic glomerulonephritis explored by network pharmacology, RNA-seq, and in vitro experiments |
| title_full | Mechanisms of Rostellularia procumbens (L.) Nees on treating chronic glomerulonephritis explored by network pharmacology, RNA-seq, and in vitro experiments |
| title_fullStr | Mechanisms of Rostellularia procumbens (L.) Nees on treating chronic glomerulonephritis explored by network pharmacology, RNA-seq, and in vitro experiments |
| title_full_unstemmed | Mechanisms of Rostellularia procumbens (L.) Nees on treating chronic glomerulonephritis explored by network pharmacology, RNA-seq, and in vitro experiments |
| title_short | Mechanisms of Rostellularia procumbens (L.) Nees on treating chronic glomerulonephritis explored by network pharmacology, RNA-seq, and in vitro experiments |
| title_sort | mechanisms of rostellularia procumbens l nees on treating chronic glomerulonephritis explored by network pharmacology rna seq and in vitro experiments |
| topic | Rostellularia procumbens (L.) Nees Chronic glomerulonephritis Network pharmacology RNA-seq Mechanism |
| url | https://doi.org/10.1186/s12906-023-04079-5 |
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