HIF-1α-mediated augmentation of miRNA-18b-5p facilitates proliferation and metastasis in osteosarcoma through attenuation PHF2
Abstract Extensive evidence has explored the involvement of microRNAs (miRNAs) in osteosarcoma (OS). Limitedly, the concrete function of microRNA-18b-5p (miR-18b-5p) in OS remains unexplored and largely elusive. Here, we validated that miR-18b-5p significantly elevated in OS via analyzing the data f...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13660-w |
| _version_ | 1811235658039033856 |
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| author | Peng Luo Yan-dong Zhang Feng He Chang-jun Tong Kai Liu He Liu Shi-zhuang Zhu Jian-zhou Luo Bing Yuan |
| author_facet | Peng Luo Yan-dong Zhang Feng He Chang-jun Tong Kai Liu He Liu Shi-zhuang Zhu Jian-zhou Luo Bing Yuan |
| author_sort | Peng Luo |
| collection | DOAJ |
| description | Abstract Extensive evidence has explored the involvement of microRNAs (miRNAs) in osteosarcoma (OS). Limitedly, the concrete function of microRNA-18b-5p (miR-18b-5p) in OS remains unexplored and largely elusive. Here, we validated that miR-18b-5p significantly elevated in OS via analyzing the data from GEO database. The results showed that miR-18b-5p was overexpressed in human OS tissues and cell lines. The clinical evidence suggested that high level of miR-18b-5p was negatively correlated with the poor prognosis of OS. Meanwhile, miR-18b-5p upregulation facilitated the proliferation and metastasis of OS cells in vitro and in vivo. The mechanism exploration demonstrated that miR-18b-5p acted as a potential inhibitor of PHF2, a tumor suppressor gene, at post-transcriptional level. Moreover, hypoxia induced gene expression of miR-18b-5p was clarified to be transcriptionally mediated by HIF-1α. The clinicopathological analysis in samples of OS patients further supported that miR-18b-5p had a positive correlation with HIF-1α expression, and negative correlation with PHF2. Collectively, the present study uncovered a new molecular mechanism of OS tumorigenesis and development and miR-18b-5p might be a prognostic biomarker and potential therapeutic target for OS treatment. |
| first_indexed | 2024-04-12T11:55:44Z |
| format | Article |
| id | doaj.art-37149339bced41d7b6f8928904a6c351 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T11:55:44Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-37149339bced41d7b6f8928904a6c3512022-12-22T03:34:01ZengNature PortfolioScientific Reports2045-23222022-06-0112111210.1038/s41598-022-13660-wHIF-1α-mediated augmentation of miRNA-18b-5p facilitates proliferation and metastasis in osteosarcoma through attenuation PHF2Peng Luo0Yan-dong Zhang1Feng He2Chang-jun Tong3Kai Liu4He Liu5Shi-zhuang Zhu6Jian-zhou Luo7Bing Yuan8Department of Orthopedics, Huazhong University of Science and Technology Union Shenzhen HospitalGuangdong Medical UniversityDepartment of Orthopedics, Huazhong University of Science and Technology Union Shenzhen HospitalDepartment of Orthopedics, Huazhong University of Science and Technology Union Shenzhen HospitalDepartment of Orthopedic Surgery, The Third Hospital of Hebei Medical UniversityDepartment of Orthopedics, Huazhong University of Science and Technology Union Shenzhen HospitalDepartment of Orthopedics, Huazhong University of Science and Technology Union Shenzhen HospitalDepartment of Orthopedics, Huazhong University of Science and Technology Union Shenzhen HospitalDepartment of Orthopedics, The Fifth Hospital of Wuhan/The Second Affiliated Hospital of Jianghan UniversityAbstract Extensive evidence has explored the involvement of microRNAs (miRNAs) in osteosarcoma (OS). Limitedly, the concrete function of microRNA-18b-5p (miR-18b-5p) in OS remains unexplored and largely elusive. Here, we validated that miR-18b-5p significantly elevated in OS via analyzing the data from GEO database. The results showed that miR-18b-5p was overexpressed in human OS tissues and cell lines. The clinical evidence suggested that high level of miR-18b-5p was negatively correlated with the poor prognosis of OS. Meanwhile, miR-18b-5p upregulation facilitated the proliferation and metastasis of OS cells in vitro and in vivo. The mechanism exploration demonstrated that miR-18b-5p acted as a potential inhibitor of PHF2, a tumor suppressor gene, at post-transcriptional level. Moreover, hypoxia induced gene expression of miR-18b-5p was clarified to be transcriptionally mediated by HIF-1α. The clinicopathological analysis in samples of OS patients further supported that miR-18b-5p had a positive correlation with HIF-1α expression, and negative correlation with PHF2. Collectively, the present study uncovered a new molecular mechanism of OS tumorigenesis and development and miR-18b-5p might be a prognostic biomarker and potential therapeutic target for OS treatment.https://doi.org/10.1038/s41598-022-13660-w |
| spellingShingle | Peng Luo Yan-dong Zhang Feng He Chang-jun Tong Kai Liu He Liu Shi-zhuang Zhu Jian-zhou Luo Bing Yuan HIF-1α-mediated augmentation of miRNA-18b-5p facilitates proliferation and metastasis in osteosarcoma through attenuation PHF2 Scientific Reports |
| title | HIF-1α-mediated augmentation of miRNA-18b-5p facilitates proliferation and metastasis in osteosarcoma through attenuation PHF2 |
| title_full | HIF-1α-mediated augmentation of miRNA-18b-5p facilitates proliferation and metastasis in osteosarcoma through attenuation PHF2 |
| title_fullStr | HIF-1α-mediated augmentation of miRNA-18b-5p facilitates proliferation and metastasis in osteosarcoma through attenuation PHF2 |
| title_full_unstemmed | HIF-1α-mediated augmentation of miRNA-18b-5p facilitates proliferation and metastasis in osteosarcoma through attenuation PHF2 |
| title_short | HIF-1α-mediated augmentation of miRNA-18b-5p facilitates proliferation and metastasis in osteosarcoma through attenuation PHF2 |
| title_sort | hif 1α mediated augmentation of mirna 18b 5p facilitates proliferation and metastasis in osteosarcoma through attenuation phf2 |
| url | https://doi.org/10.1038/s41598-022-13660-w |
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