Genetic defects in SAPK signalling, chromatin regulation, vesicle transport and CoA-related lipid metabolism are rescued by rapamycin in fission yeast
Rapamycin inhibits TOR (target of rapamycin) kinase, and is being used clinically to treat various diseases ranging from cancers to fibrodysplasia ossificans progressiva. To understand rapamycin mechanisms of action more comprehensively, 1014 temperature-sensitive (ts) fission yeast (Schizosaccharom...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The Royal Society
2018-03-01
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| Series: | Open Biology |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.170261 |
| _version_ | 1818244178990071808 |
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| author | Kenichi Sajiki Yuria Tahara Alejandro Villar-Briones Tomáš Pluskal Takayuki Teruya Ayaka Mori Mitsuko Hatanaka Masahiro Ebe Takahiro Nakamura Keita Aoki Yukinobu Nakaseko Mitsuhiro Yanagida |
| author_facet | Kenichi Sajiki Yuria Tahara Alejandro Villar-Briones Tomáš Pluskal Takayuki Teruya Ayaka Mori Mitsuko Hatanaka Masahiro Ebe Takahiro Nakamura Keita Aoki Yukinobu Nakaseko Mitsuhiro Yanagida |
| author_sort | Kenichi Sajiki |
| collection | DOAJ |
| description | Rapamycin inhibits TOR (target of rapamycin) kinase, and is being used clinically to treat various diseases ranging from cancers to fibrodysplasia ossificans progressiva. To understand rapamycin mechanisms of action more comprehensively, 1014 temperature-sensitive (ts) fission yeast (Schizosaccharomyces pombe) mutants were screened in order to isolate strains in which the ts phenotype was rescued by rapamycin. Rapamycin-rescued 45 strains, among which 12 genes responsible for temperature sensitivity were identified. These genes are involved in stress-activated protein kinase (SAPK) signalling, chromatin regulation, vesicle transport, and CoA- and mevalonate-related lipid metabolism. Subsequent metabolome analyses revealed that rapamycin upregulated stress-responsive metabolites, while it downregulated purine biosynthesis intermediates and nucleotide derivatives. Rapamycin alleviated abnormalities in cell growth and cell division caused by sty1 mutants (Δsty1) of SAPK. Notably, in Δsty1, rapamycin reduced greater than 75% of overproduced metabolites (greater than 2× WT), like purine biosynthesis intermediates and nucleotide derivatives, to WT levels. This suggests that these compounds may be the points at which the SAPK/TOR balance regulates continuous cell proliferation. Rapamycin might be therapeutically useful for specific defects of these gene functions. |
| first_indexed | 2024-12-12T14:12:54Z |
| format | Article |
| id | doaj.art-371540c6d1bd4831ba52b1813cdd9460 |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-12-12T14:12:54Z |
| publishDate | 2018-03-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj.art-371540c6d1bd4831ba52b1813cdd94602022-12-22T00:21:59ZengThe Royal SocietyOpen Biology2046-24412018-03-018310.1098/rsob.170261170261Genetic defects in SAPK signalling, chromatin regulation, vesicle transport and CoA-related lipid metabolism are rescued by rapamycin in fission yeastKenichi SajikiYuria TaharaAlejandro Villar-BrionesTomáš PluskalTakayuki TeruyaAyaka MoriMitsuko HatanakaMasahiro EbeTakahiro NakamuraKeita AokiYukinobu NakasekoMitsuhiro YanagidaRapamycin inhibits TOR (target of rapamycin) kinase, and is being used clinically to treat various diseases ranging from cancers to fibrodysplasia ossificans progressiva. To understand rapamycin mechanisms of action more comprehensively, 1014 temperature-sensitive (ts) fission yeast (Schizosaccharomyces pombe) mutants were screened in order to isolate strains in which the ts phenotype was rescued by rapamycin. Rapamycin-rescued 45 strains, among which 12 genes responsible for temperature sensitivity were identified. These genes are involved in stress-activated protein kinase (SAPK) signalling, chromatin regulation, vesicle transport, and CoA- and mevalonate-related lipid metabolism. Subsequent metabolome analyses revealed that rapamycin upregulated stress-responsive metabolites, while it downregulated purine biosynthesis intermediates and nucleotide derivatives. Rapamycin alleviated abnormalities in cell growth and cell division caused by sty1 mutants (Δsty1) of SAPK. Notably, in Δsty1, rapamycin reduced greater than 75% of overproduced metabolites (greater than 2× WT), like purine biosynthesis intermediates and nucleotide derivatives, to WT levels. This suggests that these compounds may be the points at which the SAPK/TOR balance regulates continuous cell proliferation. Rapamycin might be therapeutically useful for specific defects of these gene functions.https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.170261rapamycinsapkfission yeastmutant screeningquantitative metabolomics |
| spellingShingle | Kenichi Sajiki Yuria Tahara Alejandro Villar-Briones Tomáš Pluskal Takayuki Teruya Ayaka Mori Mitsuko Hatanaka Masahiro Ebe Takahiro Nakamura Keita Aoki Yukinobu Nakaseko Mitsuhiro Yanagida Genetic defects in SAPK signalling, chromatin regulation, vesicle transport and CoA-related lipid metabolism are rescued by rapamycin in fission yeast Open Biology rapamycin sapk fission yeast mutant screening quantitative metabolomics |
| title | Genetic defects in SAPK signalling, chromatin regulation, vesicle transport and CoA-related lipid metabolism are rescued by rapamycin in fission yeast |
| title_full | Genetic defects in SAPK signalling, chromatin regulation, vesicle transport and CoA-related lipid metabolism are rescued by rapamycin in fission yeast |
| title_fullStr | Genetic defects in SAPK signalling, chromatin regulation, vesicle transport and CoA-related lipid metabolism are rescued by rapamycin in fission yeast |
| title_full_unstemmed | Genetic defects in SAPK signalling, chromatin regulation, vesicle transport and CoA-related lipid metabolism are rescued by rapamycin in fission yeast |
| title_short | Genetic defects in SAPK signalling, chromatin regulation, vesicle transport and CoA-related lipid metabolism are rescued by rapamycin in fission yeast |
| title_sort | genetic defects in sapk signalling chromatin regulation vesicle transport and coa related lipid metabolism are rescued by rapamycin in fission yeast |
| topic | rapamycin sapk fission yeast mutant screening quantitative metabolomics |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.170261 |
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