Natural T Regulatory Cells (n Treg) in the Peripheral Blood of Healthy Subjects and Subjects with Chronic Periodontitis – A Pilot Study
Introduction: The T cells play a central role in the aetiopathogenesis of periodontal disease. Natural T regulatory cells (nTreg) are the key stone immunoregulatory elements having an anergic phenotype and play an important role in the suppression of exaggerated immune responses thereby maintai...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2016-03-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/7446/15449_CE[Ra1]_F(AK)_PF1(VSUAK)_PFA(NC_AK)_PF2(PAG).pdf |
| _version_ | 1819202388354924544 |
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| author | Ram Sabarish Suresh Ranga Rao Vamsi Lavu |
| author_facet | Ram Sabarish Suresh Ranga Rao Vamsi Lavu |
| author_sort | Ram Sabarish |
| collection | DOAJ |
| description | Introduction: The T cells play a central role in the
aetiopathogenesis of periodontal disease. Natural T regulatory
cells (nTreg) are the key stone immunoregulatory elements
having an anergic phenotype and play an important role in
the suppression of exaggerated immune responses thereby
maintaining homeostasis. There are increasing evidences for
the role of nTreg in the periodontal disease pathogenesis.
Aim: To identify the proportion of natural T regulatory cells in the
peripheral blood of periodontally healthy subjects and subjects
with chronic periodontitis.
Materials and Methods: A total of 15 subjects (7 with healthy
gingiva and 8 with chronic periodontitis) were recruited for this
pilot study. Baseline periodontal parameters were recorded and
5 ml of peripheral blood was collected. The samples from both
the groups were analysed for the relative proportion of nTreg
(identified by the expression CD45RB+CD4+CD25+FOXP3+)
using flow cytometry.
Results: The mean percentages of the CD45RB+CD4+CD25+
cells expressing FOXP3 in control and chronic periodontitis
group were found to be 14.75±5.04 and 43.13±11.17
respectively. The mean proportion of nTreg were compared
between the control and chronic periodontitis sample using
Mann-Whitney Test and was found to be statistically significant
with (p<0.001).
Conclusion: A higher proportion of nTreg in the peripheral
blood sample of chronic periodontitis subjects were observed
as compared to that of healthy individuals. |
| first_indexed | 2024-12-23T04:03:14Z |
| format | Article |
| id | doaj.art-3715efee74ec47609ecf0f4607ab7b21 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-23T04:03:14Z |
| publishDate | 2016-03-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-3715efee74ec47609ecf0f4607ab7b212022-12-21T18:00:40ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2016-03-01103ZC36ZC3910.7860/JCDR/2016/15449.7446Natural T Regulatory Cells (n Treg) in the Peripheral Blood of Healthy Subjects and Subjects with Chronic Periodontitis – A Pilot StudyRam Sabarish0Suresh Ranga Rao1Vamsi Lavu2Senior Lecturer, Department of Periodontology, Faculty of Dental Sciences, Sri Ramachandra University, Chennai, India.Professor and Head, Department of Periodontology, Faculty of Dental Sciences, Sri Ramachandra University, Chennai, India.Associate Professor, Department of Periodontology, Faculty of Dental Sciences, Sri Ramachandra University, Chennai, India.Introduction: The T cells play a central role in the aetiopathogenesis of periodontal disease. Natural T regulatory cells (nTreg) are the key stone immunoregulatory elements having an anergic phenotype and play an important role in the suppression of exaggerated immune responses thereby maintaining homeostasis. There are increasing evidences for the role of nTreg in the periodontal disease pathogenesis. Aim: To identify the proportion of natural T regulatory cells in the peripheral blood of periodontally healthy subjects and subjects with chronic periodontitis. Materials and Methods: A total of 15 subjects (7 with healthy gingiva and 8 with chronic periodontitis) were recruited for this pilot study. Baseline periodontal parameters were recorded and 5 ml of peripheral blood was collected. The samples from both the groups were analysed for the relative proportion of nTreg (identified by the expression CD45RB+CD4+CD25+FOXP3+) using flow cytometry. Results: The mean percentages of the CD45RB+CD4+CD25+ cells expressing FOXP3 in control and chronic periodontitis group were found to be 14.75±5.04 and 43.13±11.17 respectively. The mean proportion of nTreg were compared between the control and chronic periodontitis sample using Mann-Whitney Test and was found to be statistically significant with (p<0.001). Conclusion: A higher proportion of nTreg in the peripheral blood sample of chronic periodontitis subjects were observed as compared to that of healthy individuals.https://jcdr.net/articles/PDF/7446/15449_CE[Ra1]_F(AK)_PF1(VSUAK)_PFA(NC_AK)_PF2(PAG).pdfcd25foxp3periodontal diseaseregulatory t lymphocytes |
| spellingShingle | Ram Sabarish Suresh Ranga Rao Vamsi Lavu Natural T Regulatory Cells (n Treg) in the Peripheral Blood of Healthy Subjects and Subjects with Chronic Periodontitis – A Pilot Study Journal of Clinical and Diagnostic Research cd25 foxp3 periodontal disease regulatory t lymphocytes |
| title | Natural T Regulatory Cells (n Treg) in the Peripheral Blood of Healthy Subjects and Subjects with Chronic Periodontitis – A Pilot Study |
| title_full | Natural T Regulatory Cells (n Treg) in the Peripheral Blood of Healthy Subjects and Subjects with Chronic Periodontitis – A Pilot Study |
| title_fullStr | Natural T Regulatory Cells (n Treg) in the Peripheral Blood of Healthy Subjects and Subjects with Chronic Periodontitis – A Pilot Study |
| title_full_unstemmed | Natural T Regulatory Cells (n Treg) in the Peripheral Blood of Healthy Subjects and Subjects with Chronic Periodontitis – A Pilot Study |
| title_short | Natural T Regulatory Cells (n Treg) in the Peripheral Blood of Healthy Subjects and Subjects with Chronic Periodontitis – A Pilot Study |
| title_sort | natural t regulatory cells n treg in the peripheral blood of healthy subjects and subjects with chronic periodontitis a pilot study |
| topic | cd25 foxp3 periodontal disease regulatory t lymphocytes |
| url | https://jcdr.net/articles/PDF/7446/15449_CE[Ra1]_F(AK)_PF1(VSUAK)_PFA(NC_AK)_PF2(PAG).pdf |
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