Kinetoplastid Species Maintained by a Small Mammal Community in the Pantanal Biome
Kinetoplastids include species economically important in agriculture, livestock, and human health. We evaluated the richness of kinetoplastids that infect small mammals in patches of unflooded forests in the Pantanal biome, an area where we hypothesize that its diversity is higher than currently rec...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/10/1205 |
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| author | Filipe Martins Santos Nayara Yoshie Sano Sany Caroline Liberal Maria Augusta Dario Wesley Arruda Gimenes Nantes Fernanda Moreira Alves Alanderson Rodrigues da Silva Carina Elisei De Oliveira André Luiz Rodrigues Roque Heitor Miraglia Herrera Ana Maria Jansen |
| author_facet | Filipe Martins Santos Nayara Yoshie Sano Sany Caroline Liberal Maria Augusta Dario Wesley Arruda Gimenes Nantes Fernanda Moreira Alves Alanderson Rodrigues da Silva Carina Elisei De Oliveira André Luiz Rodrigues Roque Heitor Miraglia Herrera Ana Maria Jansen |
| author_sort | Filipe Martins Santos |
| collection | DOAJ |
| description | Kinetoplastids include species economically important in agriculture, livestock, and human health. We evaluated the richness of kinetoplastids that infect small mammals in patches of unflooded forests in the Pantanal biome, an area where we hypothesize that its diversity is higher than currently recognized. Hemocultures (HC) and Next Generation Sequencing (NGS) targeting the 18S rDNA gene were employed for the detection of kinetoplastids. We grouped the positive samples into pools for each small mammal species (<i>Monodelphis domestica</i>, <i>Thylamys macrurus</i>, <i>Oecomys mamorae</i>, <i>Thrichomys fosteri</i>, <i>Clyomys laticeps</i>, and <i>Holochilus chacarius</i>). Eight parasite species were identified: <i>Leishmania amazonensis</i>, <i>L. infantum</i>; <i>Trypanosoma cascavelli</i> (HC + NGS), <i>T. cruzi</i>, <i>T. lainsoni</i>, <i>T. rangeli</i> (HC + NGS), <i>Trypanosoma</i> sp. DID, and <i>Neobodo</i> sp. The use of a tool as sensitive as NGS has increased our awareness of the diversity of kinetoplastids, as well as their host range, with emphasis on the species <i>O. mamorae</i> (seven kinetoplastid species, excepting <i>T. cascavelli</i> in a pool of nine individuals) and <i>T. macrurus</i> (four kinetoplastid species in a single individual). Furthermore, <i>L. infantum</i> and <i>L. amazonensis</i> infections were described in small mammals from this region for the first time. These findings make it mandatory to revisit the kinetoplastids/host associations proposed so far. |
| first_indexed | 2024-03-09T19:37:21Z |
| format | Article |
| id | doaj.art-37160f5ef6004d49a4dea0da3ba9c42e |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T19:37:21Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-37160f5ef6004d49a4dea0da3ba9c42e2023-11-24T01:49:51ZengMDPI AGPathogens2076-08172022-10-011110120510.3390/pathogens11101205Kinetoplastid Species Maintained by a Small Mammal Community in the Pantanal BiomeFilipe Martins Santos0Nayara Yoshie Sano1Sany Caroline Liberal2Maria Augusta Dario3Wesley Arruda Gimenes Nantes4Fernanda Moreira Alves5Alanderson Rodrigues da Silva6Carina Elisei De Oliveira7André Luiz Rodrigues Roque8Heitor Miraglia Herrera9Ana Maria Jansen10Programa de Pós-Graduação em Ciências Ambientais e Sustentabilidade Agropecuária, Universidade Católica Dom Bosco (UCDB), Campo Grande 79117-010, BrazilPrograma de Pós-Graduação em Ecologia e Conservação, Universidade Federal do Mato Grosso do Sul (UFMS), Campo Grande 79070-900, BrazilPrograma de Pós-Graduação em Ciências Ambientais e Sustentabilidade Agropecuária, Universidade Católica Dom Bosco (UCDB), Campo Grande 79117-010, BrazilLaboratório de Biologia de Tripanossomatídeos, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro 21040-900, BrazilPrograma de Pós-Graduação em Ciências Ambientais e Sustentabilidade Agropecuária, Universidade Católica Dom Bosco (UCDB), Campo Grande 79117-010, BrazilLaboratório de Biologia de Tripanossomatídeos, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro 21040-900, BrazilPrograma de Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco (UCDB), Campo Grande 79117-010, BrazilPrograma de Pós-Graduação em Ciências Ambientais e Sustentabilidade Agropecuária, Universidade Católica Dom Bosco (UCDB), Campo Grande 79117-010, BrazilLaboratório de Biologia de Tripanossomatídeos, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro 21040-900, BrazilPrograma de Pós-Graduação em Ciências Ambientais e Sustentabilidade Agropecuária, Universidade Católica Dom Bosco (UCDB), Campo Grande 79117-010, BrazilPrograma de Pós-Graduação em Ciências Ambientais e Sustentabilidade Agropecuária, Universidade Católica Dom Bosco (UCDB), Campo Grande 79117-010, BrazilKinetoplastids include species economically important in agriculture, livestock, and human health. We evaluated the richness of kinetoplastids that infect small mammals in patches of unflooded forests in the Pantanal biome, an area where we hypothesize that its diversity is higher than currently recognized. Hemocultures (HC) and Next Generation Sequencing (NGS) targeting the 18S rDNA gene were employed for the detection of kinetoplastids. We grouped the positive samples into pools for each small mammal species (<i>Monodelphis domestica</i>, <i>Thylamys macrurus</i>, <i>Oecomys mamorae</i>, <i>Thrichomys fosteri</i>, <i>Clyomys laticeps</i>, and <i>Holochilus chacarius</i>). Eight parasite species were identified: <i>Leishmania amazonensis</i>, <i>L. infantum</i>; <i>Trypanosoma cascavelli</i> (HC + NGS), <i>T. cruzi</i>, <i>T. lainsoni</i>, <i>T. rangeli</i> (HC + NGS), <i>Trypanosoma</i> sp. DID, and <i>Neobodo</i> sp. The use of a tool as sensitive as NGS has increased our awareness of the diversity of kinetoplastids, as well as their host range, with emphasis on the species <i>O. mamorae</i> (seven kinetoplastid species, excepting <i>T. cascavelli</i> in a pool of nine individuals) and <i>T. macrurus</i> (four kinetoplastid species in a single individual). Furthermore, <i>L. infantum</i> and <i>L. amazonensis</i> infections were described in small mammals from this region for the first time. These findings make it mandatory to revisit the kinetoplastids/host associations proposed so far.https://www.mdpi.com/2076-0817/11/10/1205wetlandRodentiaDidelphimorphiaNext Generation SequencingAmplicon Sequence Variant |
| spellingShingle | Filipe Martins Santos Nayara Yoshie Sano Sany Caroline Liberal Maria Augusta Dario Wesley Arruda Gimenes Nantes Fernanda Moreira Alves Alanderson Rodrigues da Silva Carina Elisei De Oliveira André Luiz Rodrigues Roque Heitor Miraglia Herrera Ana Maria Jansen Kinetoplastid Species Maintained by a Small Mammal Community in the Pantanal Biome Pathogens wetland Rodentia Didelphimorphia Next Generation Sequencing Amplicon Sequence Variant |
| title | Kinetoplastid Species Maintained by a Small Mammal Community in the Pantanal Biome |
| title_full | Kinetoplastid Species Maintained by a Small Mammal Community in the Pantanal Biome |
| title_fullStr | Kinetoplastid Species Maintained by a Small Mammal Community in the Pantanal Biome |
| title_full_unstemmed | Kinetoplastid Species Maintained by a Small Mammal Community in the Pantanal Biome |
| title_short | Kinetoplastid Species Maintained by a Small Mammal Community in the Pantanal Biome |
| title_sort | kinetoplastid species maintained by a small mammal community in the pantanal biome |
| topic | wetland Rodentia Didelphimorphia Next Generation Sequencing Amplicon Sequence Variant |
| url | https://www.mdpi.com/2076-0817/11/10/1205 |
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