Validation of an Effective Protocol for <i>Culicoides</i> Latreille (Diptera: Ceratopogonidae) Detection Using eDNA Metabarcoding
eDNA metabarcoding is an effective molecular-based identification method for the biosurveillance of flighted insects. An eDNA surveillance approach maintains specimens for secondary morphological identification useful for regulatory applications. This study identified <i>Culicoides</i> s...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/5/401 |
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| author | Yoamel Milián-García Lauren A. A. Janke Robert G. Young Aruna Ambagala Robert H. Hanner |
| author_facet | Yoamel Milián-García Lauren A. A. Janke Robert G. Young Aruna Ambagala Robert H. Hanner |
| author_sort | Yoamel Milián-García |
| collection | DOAJ |
| description | eDNA metabarcoding is an effective molecular-based identification method for the biosurveillance of flighted insects. An eDNA surveillance approach maintains specimens for secondary morphological identification useful for regulatory applications. This study identified <i>Culicoides</i> species using eDNA metabarcoding and compared these results to morphological identifications of trapped specimens. Insects were collected using ultraviolet (UV) lighted fan traps containing a saturated salt (NaCl) solution from two locations in Guelph, Ontario, Canada. There were forty-two <i>Culicoides</i> specimens collected in total. Molecular identification detected four species, <i>C. biguttatus</i>, <i>C. stellifer</i>, <i>C. obsoletus,</i> and <i>C. mulrennani.</i> Using morphological identification, two out of these four taxonomic ranks were confirmed at the species level (<i>C. biguttatus</i> and <i>C. stellifer</i>) and one was confirmed at the subgenus level (<i>Avaritia</i> [<i>C. obsoletus</i>]). No molecular detection of <i>Culicoides</i> species occurred in traps with an abundance of less than three individuals per taxon. The inconsistency in identifying <i>Culicoides</i> specimens to the species level punctuates the need for curated DNA reference libraries for <i>Culicoides</i>. In conclusion, the saturated salt (NaCl) solution preserved the <i>Culicoides’</i> morphological characteristics and the eDNA. |
| first_indexed | 2024-03-10T11:47:58Z |
| format | Article |
| id | doaj.art-371ced3abec24857aaeb5d49fb4bd7f6 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T11:47:58Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-371ced3abec24857aaeb5d49fb4bd7f62023-11-21T17:56:18ZengMDPI AGInsects2075-44502021-04-0112540110.3390/insects12050401Validation of an Effective Protocol for <i>Culicoides</i> Latreille (Diptera: Ceratopogonidae) Detection Using eDNA MetabarcodingYoamel Milián-García0Lauren A. A. Janke1Robert G. Young2Aruna Ambagala3Robert H. Hanner4Department of Integrative Biology, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, CanadaDepartment of Integrative Biology, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, CanadaDepartment of Integrative Biology, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, CanadaCanadian Food Inspection Agency/Government of Canada, Winnipeg, MB R3E 3M4, CanadaDepartment of Integrative Biology, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, CanadaeDNA metabarcoding is an effective molecular-based identification method for the biosurveillance of flighted insects. An eDNA surveillance approach maintains specimens for secondary morphological identification useful for regulatory applications. This study identified <i>Culicoides</i> species using eDNA metabarcoding and compared these results to morphological identifications of trapped specimens. Insects were collected using ultraviolet (UV) lighted fan traps containing a saturated salt (NaCl) solution from two locations in Guelph, Ontario, Canada. There were forty-two <i>Culicoides</i> specimens collected in total. Molecular identification detected four species, <i>C. biguttatus</i>, <i>C. stellifer</i>, <i>C. obsoletus,</i> and <i>C. mulrennani.</i> Using morphological identification, two out of these four taxonomic ranks were confirmed at the species level (<i>C. biguttatus</i> and <i>C. stellifer</i>) and one was confirmed at the subgenus level (<i>Avaritia</i> [<i>C. obsoletus</i>]). No molecular detection of <i>Culicoides</i> species occurred in traps with an abundance of less than three individuals per taxon. The inconsistency in identifying <i>Culicoides</i> specimens to the species level punctuates the need for curated DNA reference libraries for <i>Culicoides</i>. In conclusion, the saturated salt (NaCl) solution preserved the <i>Culicoides’</i> morphological characteristics and the eDNA.https://www.mdpi.com/2075-4450/12/5/401eDNA metabarcoding<i>Culicoides</i>biosurveillancemorphologymolecular identification |
| spellingShingle | Yoamel Milián-García Lauren A. A. Janke Robert G. Young Aruna Ambagala Robert H. Hanner Validation of an Effective Protocol for <i>Culicoides</i> Latreille (Diptera: Ceratopogonidae) Detection Using eDNA Metabarcoding Insects eDNA metabarcoding <i>Culicoides</i> biosurveillance morphology molecular identification |
| title | Validation of an Effective Protocol for <i>Culicoides</i> Latreille (Diptera: Ceratopogonidae) Detection Using eDNA Metabarcoding |
| title_full | Validation of an Effective Protocol for <i>Culicoides</i> Latreille (Diptera: Ceratopogonidae) Detection Using eDNA Metabarcoding |
| title_fullStr | Validation of an Effective Protocol for <i>Culicoides</i> Latreille (Diptera: Ceratopogonidae) Detection Using eDNA Metabarcoding |
| title_full_unstemmed | Validation of an Effective Protocol for <i>Culicoides</i> Latreille (Diptera: Ceratopogonidae) Detection Using eDNA Metabarcoding |
| title_short | Validation of an Effective Protocol for <i>Culicoides</i> Latreille (Diptera: Ceratopogonidae) Detection Using eDNA Metabarcoding |
| title_sort | validation of an effective protocol for i culicoides i latreille diptera ceratopogonidae detection using edna metabarcoding |
| topic | eDNA metabarcoding <i>Culicoides</i> biosurveillance morphology molecular identification |
| url | https://www.mdpi.com/2075-4450/12/5/401 |
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