Enzymatic Characterization and Potential Application of Polygalacturonase from Trichoderma koningiopsis
A filamentous fungus producing polygalacturonase (29.2 U/mL) was isolated from orchard soil in northern Guangxi, and identified as Trichoderma koningiopsis. Using polygalacturonic acid as the substrate, the optimum reaction temperature of the enzyme was 50 ℃, and it maintained more than 70% of the m...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2023-05-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-10-027.pdf |
| _version_ | 1797805543585218560 |
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| author | YANG Chengwei, ZHU Jing, WEI Yunyi, YANG Mei, XU Wenting, CHENG Zhong |
| author_facet | YANG Chengwei, ZHU Jing, WEI Yunyi, YANG Mei, XU Wenting, CHENG Zhong |
| author_sort | YANG Chengwei, ZHU Jing, WEI Yunyi, YANG Mei, XU Wenting, CHENG Zhong |
| collection | DOAJ |
| description | A filamentous fungus producing polygalacturonase (29.2 U/mL) was isolated from orchard soil in northern Guangxi, and identified as Trichoderma koningiopsis. Using polygalacturonic acid as the substrate, the optimum reaction temperature of the enzyme was 50 ℃, and it maintained more than 70% of the maximum activity at 50 ℃ for 1 h, which was identified as a medium-high temperature tolerant enzyme. The enzyme had unique weakly acidic catalytic characteristics. The optimum reaction pH was 5.0, and it retained 73% of its maximum activity at pH 6.0, and was tolerant to pH 2.5–8.0. Zn2+ partially activated the enzyme activity, while Mn2+ inhibited the enzyme. It could depectinize five weakly acid fruit pulps (papaya, banana, banana, and white- and red-fleshed pitaya) but to different extents. The yield of juice of banana pulp increased by 24.4%, the viscosity of papaya pulp decreased by 82.5%, and the transmittance of red-fleshed pitaya pulp increased by 31.9%, indicating good depectinization efficiency for the three fruits. The polygalacturonase-producing strain has good prospects for its application in juice production from tropical and subtropical perishable fruits. |
| first_indexed | 2024-03-13T05:53:38Z |
| format | Article |
| id | doaj.art-371d329a55714e6ba63cf8f08027abb3 |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-03-13T05:53:38Z |
| publishDate | 2023-05-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-371d329a55714e6ba63cf8f08027abb32023-06-13T07:42:12ZengChina Food Publishing CompanyShipin Kexue1002-66302023-05-01441021522310.7506/spkx1002-6630-20220506-076Enzymatic Characterization and Potential Application of Polygalacturonase from Trichoderma koningiopsisYANG Chengwei, ZHU Jing, WEI Yunyi, YANG Mei, XU Wenting, CHENG Zhong0(1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, China;2. College of Food and Quality Engineering, Nanning University, Nanning 530200, China)A filamentous fungus producing polygalacturonase (29.2 U/mL) was isolated from orchard soil in northern Guangxi, and identified as Trichoderma koningiopsis. Using polygalacturonic acid as the substrate, the optimum reaction temperature of the enzyme was 50 ℃, and it maintained more than 70% of the maximum activity at 50 ℃ for 1 h, which was identified as a medium-high temperature tolerant enzyme. The enzyme had unique weakly acidic catalytic characteristics. The optimum reaction pH was 5.0, and it retained 73% of its maximum activity at pH 6.0, and was tolerant to pH 2.5–8.0. Zn2+ partially activated the enzyme activity, while Mn2+ inhibited the enzyme. It could depectinize five weakly acid fruit pulps (papaya, banana, banana, and white- and red-fleshed pitaya) but to different extents. The yield of juice of banana pulp increased by 24.4%, the viscosity of papaya pulp decreased by 82.5%, and the transmittance of red-fleshed pitaya pulp increased by 31.9%, indicating good depectinization efficiency for the three fruits. The polygalacturonase-producing strain has good prospects for its application in juice production from tropical and subtropical perishable fruits.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-10-027.pdfpolygalacturonase; trichoderma koningiopsis; identification; enzymatic properties; pulp depectinization |
| spellingShingle | YANG Chengwei, ZHU Jing, WEI Yunyi, YANG Mei, XU Wenting, CHENG Zhong Enzymatic Characterization and Potential Application of Polygalacturonase from Trichoderma koningiopsis Shipin Kexue polygalacturonase; trichoderma koningiopsis; identification; enzymatic properties; pulp depectinization |
| title | Enzymatic Characterization and Potential Application of Polygalacturonase from Trichoderma koningiopsis |
| title_full | Enzymatic Characterization and Potential Application of Polygalacturonase from Trichoderma koningiopsis |
| title_fullStr | Enzymatic Characterization and Potential Application of Polygalacturonase from Trichoderma koningiopsis |
| title_full_unstemmed | Enzymatic Characterization and Potential Application of Polygalacturonase from Trichoderma koningiopsis |
| title_short | Enzymatic Characterization and Potential Application of Polygalacturonase from Trichoderma koningiopsis |
| title_sort | enzymatic characterization and potential application of polygalacturonase from trichoderma koningiopsis |
| topic | polygalacturonase; trichoderma koningiopsis; identification; enzymatic properties; pulp depectinization |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-10-027.pdf |
| work_keys_str_mv | AT yangchengweizhujingweiyunyiyangmeixuwentingchengzhong enzymaticcharacterizationandpotentialapplicationofpolygalacturonasefromtrichodermakoningiopsis |