Methionine restriction alters bone morphology and affects osteoblast differentiation
Methionine restriction (MR) extends the lifespan of a wide variety of species, including rodents, drosophila, nematodes, and yeasts. MR has also been demonstrated to affect the overall growth of mice and rats. The objective of this study was to evaluate the effect of MR on bone structure in young an...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-12-01
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| Series: | Bone Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352187216300067 |
| _version_ | 1817970730871029760 |
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| author | Amadou Ouattara Diana Cooke Raj Gopalakrishnan Tsang-hai Huang Gene P. Ables |
| author_facet | Amadou Ouattara Diana Cooke Raj Gopalakrishnan Tsang-hai Huang Gene P. Ables |
| author_sort | Amadou Ouattara |
| collection | DOAJ |
| description | Methionine restriction (MR) extends the lifespan of a wide variety of species, including rodents, drosophila, nematodes, and yeasts. MR has also been demonstrated to affect the overall growth of mice and rats. The objective of this study was to evaluate the effect of MR on bone structure in young and aged male and female C57BL/6J mice. This study indicated that MR affected the growth rates of males and young females, but not aged females. MR reduced volumetric bone mass density (vBMD) and bone mineral content (BMC), while bone microarchitecture parameters were decreased in males and young females, but not in aged females compared to control-fed (CF) mice. However, when adjusted for bodyweight, the effect of MR in reducing vBMD, BMC and microarchitecture measurements was either attenuated or reversed suggesting that the smaller bones in MR mice is appropriate for its body size. In addition, CF and MR mice had similar intrinsic strength properties as measured by nanoindentation. Plasma biomarkers suggested that the low bone mass in MR mice could be due to increased collagen degradation, which may be influenced by leptin, IGF-1, adiponectin and FGF21 hormone levels. Mouse preosteoblast cell line cultured under low sulfur amino acid growth media attenuated gene expression levels of Col1al, Runx2, Bglap, Alpl and Spp1 suggesting delayed collagen formation and bone differentiation. Collectively, our studies revealed that MR altered bone morphology which could be mediated by delays in osteoblast differentiation. Keywords: Methionine restriction, Aged mice, Micro-computed tomography, Nanoindentation, MC3T3-E1 subclone 4 |
| first_indexed | 2024-04-13T20:37:44Z |
| format | Article |
| id | doaj.art-371f980d0ab84c05a8794f5f7951421e |
| institution | Directory Open Access Journal |
| issn | 2352-1872 |
| language | English |
| last_indexed | 2024-04-13T20:37:44Z |
| publishDate | 2016-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Bone Reports |
| spelling | doaj.art-371f980d0ab84c05a8794f5f7951421e2022-12-22T02:30:59ZengElsevierBone Reports2352-18722016-12-0153342Methionine restriction alters bone morphology and affects osteoblast differentiationAmadou Ouattara0Diana Cooke1Raj Gopalakrishnan2Tsang-hai Huang3Gene P. Ables4Orentreich Foundation for the Advancement of Science, Inc, 855 Route 301, Cold Spring, NY 10516, USAOrentreich Foundation for the Advancement of Science, Inc, 855 Route 301, Cold Spring, NY 10516, USASchool of Dentistry, University of Minnesota, Minneapolis, MN 55455, USAInstitute of Physical Education, Health and Leisure Studies, National Cheng Kung University, Tainan City, TaiwanOrentreich Foundation for the Advancement of Science, Inc, 855 Route 301, Cold Spring, NY 10516, USA; Corresponding author at: Orentreich Foundation for the Advancement of Science, Inc., 855 Route 301, Cold Spring, NY 10516, USA.Methionine restriction (MR) extends the lifespan of a wide variety of species, including rodents, drosophila, nematodes, and yeasts. MR has also been demonstrated to affect the overall growth of mice and rats. The objective of this study was to evaluate the effect of MR on bone structure in young and aged male and female C57BL/6J mice. This study indicated that MR affected the growth rates of males and young females, but not aged females. MR reduced volumetric bone mass density (vBMD) and bone mineral content (BMC), while bone microarchitecture parameters were decreased in males and young females, but not in aged females compared to control-fed (CF) mice. However, when adjusted for bodyweight, the effect of MR in reducing vBMD, BMC and microarchitecture measurements was either attenuated or reversed suggesting that the smaller bones in MR mice is appropriate for its body size. In addition, CF and MR mice had similar intrinsic strength properties as measured by nanoindentation. Plasma biomarkers suggested that the low bone mass in MR mice could be due to increased collagen degradation, which may be influenced by leptin, IGF-1, adiponectin and FGF21 hormone levels. Mouse preosteoblast cell line cultured under low sulfur amino acid growth media attenuated gene expression levels of Col1al, Runx2, Bglap, Alpl and Spp1 suggesting delayed collagen formation and bone differentiation. Collectively, our studies revealed that MR altered bone morphology which could be mediated by delays in osteoblast differentiation. Keywords: Methionine restriction, Aged mice, Micro-computed tomography, Nanoindentation, MC3T3-E1 subclone 4http://www.sciencedirect.com/science/article/pii/S2352187216300067 |
| spellingShingle | Amadou Ouattara Diana Cooke Raj Gopalakrishnan Tsang-hai Huang Gene P. Ables Methionine restriction alters bone morphology and affects osteoblast differentiation Bone Reports |
| title | Methionine restriction alters bone morphology and affects osteoblast differentiation |
| title_full | Methionine restriction alters bone morphology and affects osteoblast differentiation |
| title_fullStr | Methionine restriction alters bone morphology and affects osteoblast differentiation |
| title_full_unstemmed | Methionine restriction alters bone morphology and affects osteoblast differentiation |
| title_short | Methionine restriction alters bone morphology and affects osteoblast differentiation |
| title_sort | methionine restriction alters bone morphology and affects osteoblast differentiation |
| url | http://www.sciencedirect.com/science/article/pii/S2352187216300067 |
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