Mitochondrial dysfunction and endoplasmic reticulum stress involved in oocyte aging: an analysis using single-cell RNA-sequencing of mouse oocytes
Abstract Object To explore the mechanisms of ovarian aging, we performed overall analysis on the age-related alterations of gene expression profiles in mouse germinal vesicle (GV) stage oocytes by means of single-cell RNA-sequencing method (scRNA-seq). Methods Two age groups (5-week-old and 32-week-...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-06-01
|
| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13048-019-0529-x |
| _version_ | 1797970304942735360 |
|---|---|
| author | Tao Zhang Qingsong Xi Dan Wang Jingjing Li Meng Wang Dan Li Lixia Zhu Lei Jin |
| author_facet | Tao Zhang Qingsong Xi Dan Wang Jingjing Li Meng Wang Dan Li Lixia Zhu Lei Jin |
| author_sort | Tao Zhang |
| collection | DOAJ |
| description | Abstract Object To explore the mechanisms of ovarian aging, we performed overall analysis on the age-related alterations of gene expression profiles in mouse germinal vesicle (GV) stage oocytes by means of single-cell RNA-sequencing method (scRNA-seq). Methods Two age groups (5-week-old and 32-week-old) female KM mice were used as young and old models. Subsequently, GV oocytes were collected for scRNA-seq. The bioinformatics was performed to analyze and compare the differences of gene expression profile between GV oocytes of young and old mice. Results The analysis of scRNA-seq data showed that there were 624 differential expressed genes (DEGs) between two age groups of mouse GV stage oocytes. Four hundred forty-nine DEGs were up-regulated while 175 DEGs were down-regulated in the GV oocytes of the old group. KEGG pathway analysis revealed that the genes involved in mitochondrial function including oxidative phosphorylation and ATP production pathway were significantly down-regulated in GV oocytes of 32-week-old mice, especially the mitochondrial encoded NADH dehydrogenase (mt-Nd), including mt-Nd2, mt-Nd3, mt-Nd4, mt-Nd4L and mt-Nd5. Analysis of DEGs revealed that endoplasmic reticulum stress-related genes including AdipoR2, IRAK-1, RCAN1 and MsrB1 were significantly down-regulated in GV oocytes of 32-week-old mice. Also, analysis of DEGs demonstrated that anti-oxidation-related genes including Erbb3、Rcan1、Gsto2 and Msrb1 were significantly down-regulated in GV oocytes of old group. Conclusion The disorder of mitochondrial function, endoplasmic reticulum stress and the reduced antioxidant capability might be involved in the progression of oocyte aging. Especially, the down regulation of mitochondrial encoded subunits of respiratory chain complexes might play critical roles in the relevant mechanisms. |
| first_indexed | 2024-04-11T03:15:51Z |
| format | Article |
| id | doaj.art-3722326c96104ef5823507b0f7f8aaff |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T03:15:51Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-3722326c96104ef5823507b0f7f8aaff2023-01-02T10:31:19ZengBMCJournal of Ovarian Research1757-22152019-06-011211910.1186/s13048-019-0529-xMitochondrial dysfunction and endoplasmic reticulum stress involved in oocyte aging: an analysis using single-cell RNA-sequencing of mouse oocytesTao Zhang0Qingsong Xi1Dan Wang2Jingjing Li3Meng Wang4Dan Li5Lixia Zhu6Lei Jin7Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and TechnologyDepartment of Oncology, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and TechnologyDepartment of Ophthalmology, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and TechnologyReproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and TechnologyReproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and TechnologyReproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and TechnologyReproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and TechnologyReproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and TechnologyAbstract Object To explore the mechanisms of ovarian aging, we performed overall analysis on the age-related alterations of gene expression profiles in mouse germinal vesicle (GV) stage oocytes by means of single-cell RNA-sequencing method (scRNA-seq). Methods Two age groups (5-week-old and 32-week-old) female KM mice were used as young and old models. Subsequently, GV oocytes were collected for scRNA-seq. The bioinformatics was performed to analyze and compare the differences of gene expression profile between GV oocytes of young and old mice. Results The analysis of scRNA-seq data showed that there were 624 differential expressed genes (DEGs) between two age groups of mouse GV stage oocytes. Four hundred forty-nine DEGs were up-regulated while 175 DEGs were down-regulated in the GV oocytes of the old group. KEGG pathway analysis revealed that the genes involved in mitochondrial function including oxidative phosphorylation and ATP production pathway were significantly down-regulated in GV oocytes of 32-week-old mice, especially the mitochondrial encoded NADH dehydrogenase (mt-Nd), including mt-Nd2, mt-Nd3, mt-Nd4, mt-Nd4L and mt-Nd5. Analysis of DEGs revealed that endoplasmic reticulum stress-related genes including AdipoR2, IRAK-1, RCAN1 and MsrB1 were significantly down-regulated in GV oocytes of 32-week-old mice. Also, analysis of DEGs demonstrated that anti-oxidation-related genes including Erbb3、Rcan1、Gsto2 and Msrb1 were significantly down-regulated in GV oocytes of old group. Conclusion The disorder of mitochondrial function, endoplasmic reticulum stress and the reduced antioxidant capability might be involved in the progression of oocyte aging. Especially, the down regulation of mitochondrial encoded subunits of respiratory chain complexes might play critical roles in the relevant mechanisms.http://link.springer.com/article/10.1186/s13048-019-0529-xOvarian agingSingle-cell RNA-sequencingOocyteMitochondriaComplex IEndoplasmic reticulum stress |
| spellingShingle | Tao Zhang Qingsong Xi Dan Wang Jingjing Li Meng Wang Dan Li Lixia Zhu Lei Jin Mitochondrial dysfunction and endoplasmic reticulum stress involved in oocyte aging: an analysis using single-cell RNA-sequencing of mouse oocytes Journal of Ovarian Research Ovarian aging Single-cell RNA-sequencing Oocyte Mitochondria Complex I Endoplasmic reticulum stress |
| title | Mitochondrial dysfunction and endoplasmic reticulum stress involved in oocyte aging: an analysis using single-cell RNA-sequencing of mouse oocytes |
| title_full | Mitochondrial dysfunction and endoplasmic reticulum stress involved in oocyte aging: an analysis using single-cell RNA-sequencing of mouse oocytes |
| title_fullStr | Mitochondrial dysfunction and endoplasmic reticulum stress involved in oocyte aging: an analysis using single-cell RNA-sequencing of mouse oocytes |
| title_full_unstemmed | Mitochondrial dysfunction and endoplasmic reticulum stress involved in oocyte aging: an analysis using single-cell RNA-sequencing of mouse oocytes |
| title_short | Mitochondrial dysfunction and endoplasmic reticulum stress involved in oocyte aging: an analysis using single-cell RNA-sequencing of mouse oocytes |
| title_sort | mitochondrial dysfunction and endoplasmic reticulum stress involved in oocyte aging an analysis using single cell rna sequencing of mouse oocytes |
| topic | Ovarian aging Single-cell RNA-sequencing Oocyte Mitochondria Complex I Endoplasmic reticulum stress |
| url | http://link.springer.com/article/10.1186/s13048-019-0529-x |
| work_keys_str_mv | AT taozhang mitochondrialdysfunctionandendoplasmicreticulumstressinvolvedinoocyteagingananalysisusingsinglecellrnasequencingofmouseoocytes AT qingsongxi mitochondrialdysfunctionandendoplasmicreticulumstressinvolvedinoocyteagingananalysisusingsinglecellrnasequencingofmouseoocytes AT danwang mitochondrialdysfunctionandendoplasmicreticulumstressinvolvedinoocyteagingananalysisusingsinglecellrnasequencingofmouseoocytes AT jingjingli mitochondrialdysfunctionandendoplasmicreticulumstressinvolvedinoocyteagingananalysisusingsinglecellrnasequencingofmouseoocytes AT mengwang mitochondrialdysfunctionandendoplasmicreticulumstressinvolvedinoocyteagingananalysisusingsinglecellrnasequencingofmouseoocytes AT danli mitochondrialdysfunctionandendoplasmicreticulumstressinvolvedinoocyteagingananalysisusingsinglecellrnasequencingofmouseoocytes AT lixiazhu mitochondrialdysfunctionandendoplasmicreticulumstressinvolvedinoocyteagingananalysisusingsinglecellrnasequencingofmouseoocytes AT leijin mitochondrialdysfunctionandendoplasmicreticulumstressinvolvedinoocyteagingananalysisusingsinglecellrnasequencingofmouseoocytes |