Mito-SiPE is a sequence-independent and PCR-free mtDNA enrichment method for accurate ultra-deep mitochondrial sequencing
Mito-SiPE is a mitochondrial DNA (mtDNA) enrichment method that can derive high-quality mtDNA for rare heteroplasmy analysis, without relying on PCR or probes.
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-022-04182-2 |
| _version_ | 1811309173251506176 |
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| author | Darren J. Walsh David J. Bernard Faith Pangilinan Madison Esposito Denise Harold Anne Parle-McDermott Lawrence C. Brody |
| author_facet | Darren J. Walsh David J. Bernard Faith Pangilinan Madison Esposito Denise Harold Anne Parle-McDermott Lawrence C. Brody |
| author_sort | Darren J. Walsh |
| collection | DOAJ |
| description | Mito-SiPE is a mitochondrial DNA (mtDNA) enrichment method that can derive high-quality mtDNA for rare heteroplasmy analysis, without relying on PCR or probes. |
| first_indexed | 2024-04-13T09:37:38Z |
| format | Article |
| id | doaj.art-3722e77dd15e456480920ed7e9feff47 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-04-13T09:37:38Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-3722e77dd15e456480920ed7e9feff472022-12-22T02:52:02ZengNature PortfolioCommunications Biology2399-36422022-11-015111110.1038/s42003-022-04182-2Mito-SiPE is a sequence-independent and PCR-free mtDNA enrichment method for accurate ultra-deep mitochondrial sequencingDarren J. Walsh0David J. Bernard1Faith Pangilinan2Madison Esposito3Denise Harold4Anne Parle-McDermott5Lawrence C. Brody6Gene and Environment Interaction Section, National Human Genome Research Institute, NIHGene and Environment Interaction Section, National Human Genome Research Institute, NIHGene and Environment Interaction Section, National Human Genome Research Institute, NIHGene and Environment Interaction Section, National Human Genome Research Institute, NIHSchool of Biotechnology, Dublin City UniversitySchool of Biotechnology, Dublin City UniversityGene and Environment Interaction Section, National Human Genome Research Institute, NIHMito-SiPE is a mitochondrial DNA (mtDNA) enrichment method that can derive high-quality mtDNA for rare heteroplasmy analysis, without relying on PCR or probes.https://doi.org/10.1038/s42003-022-04182-2 |
| spellingShingle | Darren J. Walsh David J. Bernard Faith Pangilinan Madison Esposito Denise Harold Anne Parle-McDermott Lawrence C. Brody Mito-SiPE is a sequence-independent and PCR-free mtDNA enrichment method for accurate ultra-deep mitochondrial sequencing Communications Biology |
| title | Mito-SiPE is a sequence-independent and PCR-free mtDNA enrichment method for accurate ultra-deep mitochondrial sequencing |
| title_full | Mito-SiPE is a sequence-independent and PCR-free mtDNA enrichment method for accurate ultra-deep mitochondrial sequencing |
| title_fullStr | Mito-SiPE is a sequence-independent and PCR-free mtDNA enrichment method for accurate ultra-deep mitochondrial sequencing |
| title_full_unstemmed | Mito-SiPE is a sequence-independent and PCR-free mtDNA enrichment method for accurate ultra-deep mitochondrial sequencing |
| title_short | Mito-SiPE is a sequence-independent and PCR-free mtDNA enrichment method for accurate ultra-deep mitochondrial sequencing |
| title_sort | mito sipe is a sequence independent and pcr free mtdna enrichment method for accurate ultra deep mitochondrial sequencing |
| url | https://doi.org/10.1038/s42003-022-04182-2 |
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