First Characterization of The Venom from <i>Apis mellifera syriaca</i>, A Honeybee from The Middle East Region
Bee venom is a mixture of several components with proven therapeutic benefits, among which are anti-inflammatory, analgesic, and various cardiovascular conditions. In this work, we analyzed for the first time the proteomic content and biological properties of the crude venom from <i>Apis melli...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-03-01
|
| Series: | Toxins |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6651/11/4/191 |
| _version_ | 1798003745928249344 |
|---|---|
| author | Jacinthe Frangieh Yahya Salma Katia Haddad Cesar Mattei Christian Legros Ziad Fajloun Dany El Obeid |
| author_facet | Jacinthe Frangieh Yahya Salma Katia Haddad Cesar Mattei Christian Legros Ziad Fajloun Dany El Obeid |
| author_sort | Jacinthe Frangieh |
| collection | DOAJ |
| description | Bee venom is a mixture of several components with proven therapeutic benefits, among which are anti-inflammatory, analgesic, and various cardiovascular conditions. In this work, we analyzed for the first time the proteomic content and biological properties of the crude venom from <i>Apis mellifera syriaca</i>, a honeybee from the Middle East region. Using high-performance liquid chromatography-tandem mass spectrometry, we evidence the venom contains phospholipase A2, hyaluronidase, mast cell-degranulating peptide, adolapin, apamin, and melittin. The latter was purified by solid phase extraction method (SPE) and tested in parallel with crude venom for biological activities. Precisely, crude venom—but not melittin—exhibited antibacterial activity against <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> strains. Alongside, hemolytic activity was observed in human blood subjected to the venom at high doses. <i>A. mellifera syriaca</i> venom displayed antioxidant activities, and not surprisingly, PLA2 catalytic activity. Eventually, the venom proved to exert antiproliferative effects against MCF-7 and 3T3 cancer cells lines. This first report of a new bee venom opens new avenues for therapeutic uses of bee venoms. |
| first_indexed | 2024-04-11T12:12:38Z |
| format | Article |
| id | doaj.art-3723f224c38642ef8c2f233cc8e3b293 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T12:12:38Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-3723f224c38642ef8c2f233cc8e3b2932022-12-22T04:24:33ZengMDPI AGToxins2072-66512019-03-0111419110.3390/toxins11040191toxins11040191First Characterization of The Venom from <i>Apis mellifera syriaca</i>, A Honeybee from The Middle East RegionJacinthe Frangieh0Yahya Salma1Katia Haddad2Cesar Mattei3Christian Legros4Ziad Fajloun5Dany El Obeid6Laboratory of Applied Biotechnology (LBA3B), Azm Center for Research in Biotechnology and its Applications, EDST, Lebanese University, Tripoli 1300, LebanonLaboratory of Applied Biotechnology (LBA3B), Azm Center for Research in Biotechnology and its Applications, EDST, Lebanese University, Tripoli 1300, LebanonFaculty of Sciences 3, Lebanese University, Michel Slayman Tripoli Campus, Ras Maska 1352, LebanonMitochondrial and Cardiovascular Pathophysiology – MITOVASC, Team 2, Cardiovascular Mechanotransduction, UMR CNRS 6015, INSERM U1083, Angers University, 49045 Angers, FranceMitochondrial and Cardiovascular Pathophysiology – MITOVASC, Team 2, Cardiovascular Mechanotransduction, UMR CNRS 6015, INSERM U1083, Angers University, 49045 Angers, FranceLaboratory of Applied Biotechnology (LBA3B), Azm Center for Research in Biotechnology and its Applications, EDST, Lebanese University, Tripoli 1300, LebanonFaculty of Agriculture & Veterinary Sciences, Lebanese University, Dekwaneh, Beirut 2832, LebanonBee venom is a mixture of several components with proven therapeutic benefits, among which are anti-inflammatory, analgesic, and various cardiovascular conditions. In this work, we analyzed for the first time the proteomic content and biological properties of the crude venom from <i>Apis mellifera syriaca</i>, a honeybee from the Middle East region. Using high-performance liquid chromatography-tandem mass spectrometry, we evidence the venom contains phospholipase A2, hyaluronidase, mast cell-degranulating peptide, adolapin, apamin, and melittin. The latter was purified by solid phase extraction method (SPE) and tested in parallel with crude venom for biological activities. Precisely, crude venom—but not melittin—exhibited antibacterial activity against <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> strains. Alongside, hemolytic activity was observed in human blood subjected to the venom at high doses. <i>A. mellifera syriaca</i> venom displayed antioxidant activities, and not surprisingly, PLA2 catalytic activity. Eventually, the venom proved to exert antiproliferative effects against MCF-7 and 3T3 cancer cells lines. This first report of a new bee venom opens new avenues for therapeutic uses of bee venoms.https://www.mdpi.com/2072-6651/11/4/191<i>Apis mellifera syriaca</i>bee venommelittinLC-ESI-MSsolid phase extractionin vitro effects |
| spellingShingle | Jacinthe Frangieh Yahya Salma Katia Haddad Cesar Mattei Christian Legros Ziad Fajloun Dany El Obeid First Characterization of The Venom from <i>Apis mellifera syriaca</i>, A Honeybee from The Middle East Region Toxins <i>Apis mellifera syriaca</i> bee venom melittin LC-ESI-MS solid phase extraction in vitro effects |
| title | First Characterization of The Venom from <i>Apis mellifera syriaca</i>, A Honeybee from The Middle East Region |
| title_full | First Characterization of The Venom from <i>Apis mellifera syriaca</i>, A Honeybee from The Middle East Region |
| title_fullStr | First Characterization of The Venom from <i>Apis mellifera syriaca</i>, A Honeybee from The Middle East Region |
| title_full_unstemmed | First Characterization of The Venom from <i>Apis mellifera syriaca</i>, A Honeybee from The Middle East Region |
| title_short | First Characterization of The Venom from <i>Apis mellifera syriaca</i>, A Honeybee from The Middle East Region |
| title_sort | first characterization of the venom from i apis mellifera syriaca i a honeybee from the middle east region |
| topic | <i>Apis mellifera syriaca</i> bee venom melittin LC-ESI-MS solid phase extraction in vitro effects |
| url | https://www.mdpi.com/2072-6651/11/4/191 |
| work_keys_str_mv | AT jacinthefrangieh firstcharacterizationofthevenomfromiapismelliferasyriacaiahoneybeefromthemiddleeastregion AT yahyasalma firstcharacterizationofthevenomfromiapismelliferasyriacaiahoneybeefromthemiddleeastregion AT katiahaddad firstcharacterizationofthevenomfromiapismelliferasyriacaiahoneybeefromthemiddleeastregion AT cesarmattei firstcharacterizationofthevenomfromiapismelliferasyriacaiahoneybeefromthemiddleeastregion AT christianlegros firstcharacterizationofthevenomfromiapismelliferasyriacaiahoneybeefromthemiddleeastregion AT ziadfajloun firstcharacterizationofthevenomfromiapismelliferasyriacaiahoneybeefromthemiddleeastregion AT danyelobeid firstcharacterizationofthevenomfromiapismelliferasyriacaiahoneybeefromthemiddleeastregion |