Differentiation and expansion of endothelial cells requires pre-optimization of KDR+ expression kinetics
Human endothelial cells (ECs) are important tools in research and development of new therapies in the fields of angiogenesis, vasculogenesis, engineering organoids and multicellular tissues, drug discovery, and disease modeling. Efficient and robust induction of ECs from human pluripotent stem cells...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-01-01
|
| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506119303150 |
| _version_ | 1819178642725404672 |
|---|---|
| author | Basharat Jahan Kara E. McCloskey |
| author_facet | Basharat Jahan Kara E. McCloskey |
| author_sort | Basharat Jahan |
| collection | DOAJ |
| description | Human endothelial cells (ECs) are important tools in research and development of new therapies in the fields of angiogenesis, vasculogenesis, engineering organoids and multicellular tissues, drug discovery, and disease modeling. Efficient and robust induction of ECs from human pluripotent stem cells (hPSCs) serve as a renewable and indefinite cell sources. However, individual lines of embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) are distinct and can often respond very differently to the same microenvironmental cues. Therefore, we set out to develop a differentiation methodology specifically designed for robustness across multiple human iPSC lines. In general, the key soluble signals remain consistent across cell lines, but because the differentiation and proliferation kinetics can differ slightly in hESC and iPSC cell lines, the time point for KDR+ cell sorting must be pre-determined for each cell line. This three-stage induction method uses three different chemically defined medium formulations and generates highly purified populations of actively proliferating and functional VE-cadherin+ ECs within 30 days. Keywords: Cell fate, Differentiation, Stem cells, Vascular progenitor cells, Endothelial cells |
| first_indexed | 2024-12-22T21:45:48Z |
| format | Article |
| id | doaj.art-3728242e392240b5a12f918d629b049d |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-22T21:45:48Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-3728242e392240b5a12f918d629b049d2022-12-21T18:11:30ZengElsevierStem Cell Research1873-50612020-01-0142Differentiation and expansion of endothelial cells requires pre-optimization of KDR+ expression kineticsBasharat Jahan0Kara E. McCloskey1Graduate Program in Biological Engineering and Small-scale Technologies, University of California, Merced, United StatesGraduate Program in Biological Engineering and Small-scale Technologies, University of California, Merced, United States; Department of Materials Science and Engineering, University of California, Merced, United States; Corresponding author at: School of Engineering, Materials Science and Engineering Departments, University of California, Merced, 5200N. Lake Rd., Merced, CA 95343, United States.Human endothelial cells (ECs) are important tools in research and development of new therapies in the fields of angiogenesis, vasculogenesis, engineering organoids and multicellular tissues, drug discovery, and disease modeling. Efficient and robust induction of ECs from human pluripotent stem cells (hPSCs) serve as a renewable and indefinite cell sources. However, individual lines of embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) are distinct and can often respond very differently to the same microenvironmental cues. Therefore, we set out to develop a differentiation methodology specifically designed for robustness across multiple human iPSC lines. In general, the key soluble signals remain consistent across cell lines, but because the differentiation and proliferation kinetics can differ slightly in hESC and iPSC cell lines, the time point for KDR+ cell sorting must be pre-determined for each cell line. This three-stage induction method uses three different chemically defined medium formulations and generates highly purified populations of actively proliferating and functional VE-cadherin+ ECs within 30 days. Keywords: Cell fate, Differentiation, Stem cells, Vascular progenitor cells, Endothelial cellshttp://www.sciencedirect.com/science/article/pii/S1873506119303150 |
| spellingShingle | Basharat Jahan Kara E. McCloskey Differentiation and expansion of endothelial cells requires pre-optimization of KDR+ expression kinetics Stem Cell Research |
| title | Differentiation and expansion of endothelial cells requires pre-optimization of KDR+ expression kinetics |
| title_full | Differentiation and expansion of endothelial cells requires pre-optimization of KDR+ expression kinetics |
| title_fullStr | Differentiation and expansion of endothelial cells requires pre-optimization of KDR+ expression kinetics |
| title_full_unstemmed | Differentiation and expansion of endothelial cells requires pre-optimization of KDR+ expression kinetics |
| title_short | Differentiation and expansion of endothelial cells requires pre-optimization of KDR+ expression kinetics |
| title_sort | differentiation and expansion of endothelial cells requires pre optimization of kdr expression kinetics |
| url | http://www.sciencedirect.com/science/article/pii/S1873506119303150 |
| work_keys_str_mv | AT basharatjahan differentiationandexpansionofendothelialcellsrequirespreoptimizationofkdrexpressionkinetics AT karaemccloskey differentiationandexpansionofendothelialcellsrequirespreoptimizationofkdrexpressionkinetics |