Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress
Objective(s): Obstructive sleep apnea (OSA) is confirmed to cause lesions in multiple organs, especially in the lung tissue. Tempol is an antioxidant that has been reported to restrain inflammation and oxidative stress, with its role in OSA-induced lung injury being unclear. This study aimed to inve...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2018-12-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_11687_cb5e74d70974d423567cf6e0c165a7a8.pdf |
| _version_ | 1818208954221592576 |
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| author | Yeying Wang Bing Hai Li Ai Yu Cao Ran Li Hui Li Yongxia Li |
| author_facet | Yeying Wang Bing Hai Li Ai Yu Cao Ran Li Hui Li Yongxia Li |
| author_sort | Yeying Wang |
| collection | DOAJ |
| description | Objective(s): Obstructive sleep apnea (OSA) is confirmed to cause lesions in multiple organs, especially in the lung tissue. Tempol is an antioxidant that has been reported to restrain inflammation and oxidative stress, with its role in OSA-induced lung injury being unclear. This study aimed to investigate the beneficial effect of tempol on chronic intermittent hypoxia (IH)-induced lung injury.Materials and Methods: A rat model of OSA was established by IH. There were four groups: normal air (NA), IH, IH+tempol, NA+tempol. Inflammatory response was evaluated by TNF-α, IL-1β, and IL-6 levels. Oxidative stress was detected by MDA and GSH levels, and SOD activity. The protein levels were assessed by Western blot. DNA binding activity of NF-κB or Nrf2 was determined by electrophoretic mobility shift assay.Results: According to the results, tempol administration alleviated pathological changes of the lung tissue, decreased leukocyte count and protein content (P |
| first_indexed | 2024-12-12T04:53:01Z |
| format | Article |
| id | doaj.art-372b573a698641c8bd591982f3d5ca61 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-12T04:53:01Z |
| publishDate | 2018-12-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-372b573a698641c8bd591982f3d5ca612022-12-22T00:37:26ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742018-12-0121121238124410.22038/ijbms.2018.31716.771411687Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stressYeying Wang0Bing Hai1Li Ai2Yu Cao3Ran Li4Hui Li5Yongxia Li6Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, People’s Republic of China|Department of Epidemiology and Biostatistics, School of Public Health, Kunming Medical University, Kunming, Yunnan 650500, People’s Republic of ChinaDepartment of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, People’s Republic of ChinaDepartment of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, People’s Republic of ChinaDepartment of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, People’s Republic of ChinaDepartment of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, People’s Republic of ChinaDepartment of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, People’s Republic of ChinaDepartment of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, People’s Republic of ChinaObjective(s): Obstructive sleep apnea (OSA) is confirmed to cause lesions in multiple organs, especially in the lung tissue. Tempol is an antioxidant that has been reported to restrain inflammation and oxidative stress, with its role in OSA-induced lung injury being unclear. This study aimed to investigate the beneficial effect of tempol on chronic intermittent hypoxia (IH)-induced lung injury.Materials and Methods: A rat model of OSA was established by IH. There were four groups: normal air (NA), IH, IH+tempol, NA+tempol. Inflammatory response was evaluated by TNF-α, IL-1β, and IL-6 levels. Oxidative stress was detected by MDA and GSH levels, and SOD activity. The protein levels were assessed by Western blot. DNA binding activity of NF-κB or Nrf2 was determined by electrophoretic mobility shift assay.Results: According to the results, tempol administration alleviated pathological changes of the lung tissue, decreased leukocyte count and protein content (Phttp://ijbms.mums.ac.ir/article_11687_cb5e74d70974d423567cf6e0c165a7a8.pdfInflammation responseIntermittent hypoxiaNF-κBNrf2/HO-1Oxidative stressTempol |
| spellingShingle | Yeying Wang Bing Hai Li Ai Yu Cao Ran Li Hui Li Yongxia Li Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress Iranian Journal of Basic Medical Sciences Inflammation response Intermittent hypoxia NF-κB Nrf2/HO-1 Oxidative stress Tempol |
| title | Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress |
| title_full | Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress |
| title_fullStr | Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress |
| title_full_unstemmed | Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress |
| title_short | Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress |
| title_sort | tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress |
| topic | Inflammation response Intermittent hypoxia NF-κB Nrf2/HO-1 Oxidative stress Tempol |
| url | http://ijbms.mums.ac.ir/article_11687_cb5e74d70974d423567cf6e0c165a7a8.pdf |
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