The Spread of Beta Lactam Antibiotic-Resistant Staphylococcus aureus and Staphylococcus epidermidis Strains in Hospital
Bachground and objectives: Hospital surfaces can serve as reservoirs of potential pathogen bacteria. Staff hands are the most important source of transmission in hospital. The prevalence of β–lactamase producer bacteria in staff hands and hospital surfaces, increase antibiotic resistance nosocomial...
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| Format: | Article |
| Language: | English |
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Golestan University of Medical Sciences
2010-10-01
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| Series: | Medical Laboratory Journal |
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| Online Access: | http://mlj.goums.ac.ir/article-1-89-en.html |
| _version_ | 1818997434825572352 |
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| author | SH Jalalpoor |
| author_facet | SH Jalalpoor |
| author_sort | SH Jalalpoor |
| collection | DOAJ |
| description | Bachground and objectives: Hospital surfaces can serve as reservoirs of potential pathogen bacteria. Staff hands are the most important source of transmission in hospital. The prevalence of β–lactamase producer bacteria in staff hands and hospital surfaces, increase antibiotic resistance nosocomial infection. The aim of this study was to survey the spread of beta-lactam resistance Staphylococcus sp. in Al-zahar hospital in Isfahan. Material and Methods: The research was carried out during 2005-2007 years in Al-zahra hospital in Isfahan. Overall, 274 samples (194 strains from surface and 80 strains from staff hands).The surface samples collected by using swab in Nutrient Broth (NB) and staff hand samples collected using Finger Print method. Bacterial identification was performed by Bacteriological methods, β–lactamase production by acidimetric method and antibiogram pattern by Kirby Bauer method. Results: Of 194 strains isolated from hospital surfaces, 105 (53.7%) strains, and of 80 strains isolated from staff hands, 28 (35%) are related to Staphylococcus sp. According to acidimetric test, 79.8% of S.aureus strains and 68.55% of S.epidermidis strains produce β-lactamase. Conclusion: Results show high frequency of antibiotic resistance and β– lactamase producer Staphylococcus sp. on staff hands and hospital surfaces. Reduction of bacteria in these sources is the most important manner to control transfer of virulence agents in bacteria and create of antibioticresistant strains. Key words: Staphylococcus aureus, Staphylococcus epidermidis, β– lactamase, Antibiotic-resistant, Hospital surfaces, staff hands. |
| first_indexed | 2024-12-20T21:45:35Z |
| format | Article |
| id | doaj.art-372b57bba7da4794a58756c0d479033a |
| institution | Directory Open Access Journal |
| issn | 2538-4449 |
| language | English |
| last_indexed | 2024-12-20T21:45:35Z |
| publishDate | 2010-10-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | Medical Laboratory Journal |
| spelling | doaj.art-372b57bba7da4794a58756c0d479033a2022-12-21T19:25:41ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492010-10-01426066The Spread of Beta Lactam Antibiotic-Resistant Staphylococcus aureus and Staphylococcus epidermidis Strains in HospitalSH Jalalpoor0 Lecture of Microbiology, Islamic Bachground and objectives: Hospital surfaces can serve as reservoirs of potential pathogen bacteria. Staff hands are the most important source of transmission in hospital. The prevalence of β–lactamase producer bacteria in staff hands and hospital surfaces, increase antibiotic resistance nosocomial infection. The aim of this study was to survey the spread of beta-lactam resistance Staphylococcus sp. in Al-zahar hospital in Isfahan. Material and Methods: The research was carried out during 2005-2007 years in Al-zahra hospital in Isfahan. Overall, 274 samples (194 strains from surface and 80 strains from staff hands).The surface samples collected by using swab in Nutrient Broth (NB) and staff hand samples collected using Finger Print method. Bacterial identification was performed by Bacteriological methods, β–lactamase production by acidimetric method and antibiogram pattern by Kirby Bauer method. Results: Of 194 strains isolated from hospital surfaces, 105 (53.7%) strains, and of 80 strains isolated from staff hands, 28 (35%) are related to Staphylococcus sp. According to acidimetric test, 79.8% of S.aureus strains and 68.55% of S.epidermidis strains produce β-lactamase. Conclusion: Results show high frequency of antibiotic resistance and β– lactamase producer Staphylococcus sp. on staff hands and hospital surfaces. Reduction of bacteria in these sources is the most important manner to control transfer of virulence agents in bacteria and create of antibioticresistant strains. Key words: Staphylococcus aureus, Staphylococcus epidermidis, β– lactamase, Antibiotic-resistant, Hospital surfaces, staff hands.http://mlj.goums.ac.ir/article-1-89-en.htmlstaphylococcus aureusstaphylococcus epidermidisβ–lactamaseantibiotic-resistanthospital surfacesstaff hands. |
| spellingShingle | SH Jalalpoor The Spread of Beta Lactam Antibiotic-Resistant Staphylococcus aureus and Staphylococcus epidermidis Strains in Hospital Medical Laboratory Journal staphylococcus aureus staphylococcus epidermidis β–lactamase antibiotic-resistant hospital surfaces staff hands. |
| title | The Spread of Beta Lactam Antibiotic-Resistant Staphylococcus aureus and Staphylococcus epidermidis Strains in Hospital |
| title_full | The Spread of Beta Lactam Antibiotic-Resistant Staphylococcus aureus and Staphylococcus epidermidis Strains in Hospital |
| title_fullStr | The Spread of Beta Lactam Antibiotic-Resistant Staphylococcus aureus and Staphylococcus epidermidis Strains in Hospital |
| title_full_unstemmed | The Spread of Beta Lactam Antibiotic-Resistant Staphylococcus aureus and Staphylococcus epidermidis Strains in Hospital |
| title_short | The Spread of Beta Lactam Antibiotic-Resistant Staphylococcus aureus and Staphylococcus epidermidis Strains in Hospital |
| title_sort | spread of beta lactam antibiotic resistant staphylococcus aureus and staphylococcus epidermidis strains in hospital |
| topic | staphylococcus aureus staphylococcus epidermidis β–lactamase antibiotic-resistant hospital surfaces staff hands. |
| url | http://mlj.goums.ac.ir/article-1-89-en.html |
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