Soluble lectin-like oxidized low density lipoprotein receptor-1 in type 2 diabetes mellitus
The lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) can be proteolytically cleaved and released as soluble forms (sLOX-1). We have determined serums LOX-1 in type 2 diabetes and evaluated the effect of glucose and advanced glycation end products (AGEs) on sLOX-1 in vitro and in vivo....
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| Format: | Article |
| Language: | English |
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Elsevier
2008-07-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520347155 |
| _version_ | 1818690928081108992 |
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| author | Kathryn C.B. Tan Sammy W.M. Shiu Ying Wong Lin Leng Richard Bucala |
| author_facet | Kathryn C.B. Tan Sammy W.M. Shiu Ying Wong Lin Leng Richard Bucala |
| author_sort | Kathryn C.B. Tan |
| collection | DOAJ |
| description | The lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) can be proteolytically cleaved and released as soluble forms (sLOX-1). We have determined serums LOX-1 in type 2 diabetes and evaluated the effect of glucose and advanced glycation end products (AGEs) on sLOX-1 in vitro and in vivo. Endothelial cells were incubated with glucose or AGEs, and sLOX-1 in cell medium was measured. Serum sLOX-1 was measured in 219 diabetic patients and 187 controls by ELISA. The effect of lowering glucose and AGEs on sLOX-1 was determined in 38 poorly controlled diabetic patients after improvement in glycemic control. Incubation of endothelial cells with AGE-BSA led to a dose-dependent increase in sLOX-1, whereas the effect of glucose on sLOX-1 was less marked. Serum sLOX-1 was 9% higher in diabetic patients compared with controls (P < 0.01). In the poorly controlled patients, serum sLOX-1 decreased by 12.5% after improvement in glycemic control (P < 0.05). The magnitude of reduction in sLOX-1 correlated with the improvement in hemoglobin A1c and AGEs but not with the reduction in oxidized LDL.XXX sLOX-1 level is increased in type 2 diabetes. Both glucose and AGEs are important determinants of LOX-1 expression, and lowering glucose and AGEs leads to a reduction in sLOX-1. |
| first_indexed | 2024-12-17T12:33:47Z |
| format | Article |
| id | doaj.art-37301186e2f74819b35a51feced00985 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T12:33:47Z |
| publishDate | 2008-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-37301186e2f74819b35a51feced009852022-12-21T21:48:27ZengElsevierJournal of Lipid Research0022-22752008-07-0149714381444Soluble lectin-like oxidized low density lipoprotein receptor-1 in type 2 diabetes mellitusKathryn C.B. Tan0Sammy W.M. Shiu1Ying Wong2Lin Leng3Richard Bucala4Department of Medicine, University of Hong Kong, Hong Kong, ChinaDepartment of Medicine, University of Hong Kong, Hong Kong, ChinaDepartment of Medicine, University of Hong Kong, Hong Kong, ChinaDepartment of Medicine and Pathology, Yale University School of Medicine, New Haven, CTDepartment of Medicine and Pathology, Yale University School of Medicine, New Haven, CTThe lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) can be proteolytically cleaved and released as soluble forms (sLOX-1). We have determined serums LOX-1 in type 2 diabetes and evaluated the effect of glucose and advanced glycation end products (AGEs) on sLOX-1 in vitro and in vivo. Endothelial cells were incubated with glucose or AGEs, and sLOX-1 in cell medium was measured. Serum sLOX-1 was measured in 219 diabetic patients and 187 controls by ELISA. The effect of lowering glucose and AGEs on sLOX-1 was determined in 38 poorly controlled diabetic patients after improvement in glycemic control. Incubation of endothelial cells with AGE-BSA led to a dose-dependent increase in sLOX-1, whereas the effect of glucose on sLOX-1 was less marked. Serum sLOX-1 was 9% higher in diabetic patients compared with controls (P < 0.01). In the poorly controlled patients, serum sLOX-1 decreased by 12.5% after improvement in glycemic control (P < 0.05). The magnitude of reduction in sLOX-1 correlated with the improvement in hemoglobin A1c and AGEs but not with the reduction in oxidized LDL.XXX sLOX-1 level is increased in type 2 diabetes. Both glucose and AGEs are important determinants of LOX-1 expression, and lowering glucose and AGEs leads to a reduction in sLOX-1.http://www.sciencedirect.com/science/article/pii/S0022227520347155advanced glycation end productsoxidized LDLsoluble receptors |
| spellingShingle | Kathryn C.B. Tan Sammy W.M. Shiu Ying Wong Lin Leng Richard Bucala Soluble lectin-like oxidized low density lipoprotein receptor-1 in type 2 diabetes mellitus Journal of Lipid Research advanced glycation end products oxidized LDL soluble receptors |
| title | Soluble lectin-like oxidized low density lipoprotein receptor-1 in type 2 diabetes mellitus |
| title_full | Soluble lectin-like oxidized low density lipoprotein receptor-1 in type 2 diabetes mellitus |
| title_fullStr | Soluble lectin-like oxidized low density lipoprotein receptor-1 in type 2 diabetes mellitus |
| title_full_unstemmed | Soluble lectin-like oxidized low density lipoprotein receptor-1 in type 2 diabetes mellitus |
| title_short | Soluble lectin-like oxidized low density lipoprotein receptor-1 in type 2 diabetes mellitus |
| title_sort | soluble lectin like oxidized low density lipoprotein receptor 1 in type 2 diabetes mellitus |
| topic | advanced glycation end products oxidized LDL soluble receptors |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520347155 |
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