Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice
Abstract Background Retina diseases may lead to blindness as they often afflict both eyes. Stem cell transplantation into the affected eye(s) is a promising therapeutic strategy for certain retinal diseases. Human peripheral blood mononuclear cells (hPBMCs) are a good source of stem cells, but it is...
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| Format: | Article |
| Language: | English |
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BMC
2021-03-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13287-021-02180-5 |
| _version_ | 1818610463680757760 |
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| author | Jianfa Huang Bikun Xian Yuting Peng Baozhu Zeng Weihua Li Zhiquan Li Yaojue Xie Minglei Zhao Hening Zhang Minyi Zhou Huan Yu Peixin Wu Xing Liu Bing Huang |
| author_facet | Jianfa Huang Bikun Xian Yuting Peng Baozhu Zeng Weihua Li Zhiquan Li Yaojue Xie Minglei Zhao Hening Zhang Minyi Zhou Huan Yu Peixin Wu Xing Liu Bing Huang |
| author_sort | Jianfa Huang |
| collection | DOAJ |
| description | Abstract Background Retina diseases may lead to blindness as they often afflict both eyes. Stem cell transplantation into the affected eye(s) is a promising therapeutic strategy for certain retinal diseases. Human peripheral blood mononuclear cells (hPBMCs) are a good source of stem cells, but it is unclear whether pre-induced hPBMCs can migrate from the injected eye to the contralateral eye for bilateral treatment. We examine the possibility of bilateral cell transplantation from unilateral cell injection. Methods One hundred and sixty-one 3-month-old retinal degeneration 1 (rd1) mice were divided randomly into 3 groups: an untreated group (n = 45), a control group receiving serum-free Dulbecco’s modified Eagle’s medium (DMEM) injection into the right subretina (n = 45), and a treatment group receiving injection of pre-induced hPBMCs into the right subretina (n = 71). Both eyes were examined by full-field electroretinogram (ERG), immunofluorescence, flow cytometry, and quantitative real-time polymerase chain reaction (qRT-PCR) at 1 and 3 months post-injection. Results At both 1 and 3 months post-injection, labeled pre-induced hPBMCs were observed in the retinal inner nuclear layer of the contralateral (left untreated) eye as well as the treated eye as evidenced by immunofluorescence staining for a human antigen. Flow cytometry of fluorescently label cells and qRT-PCR of hPBMCs genes confirmed that transplanted hPBMCs migrated from the treated to the contralateral untreated eye and remained viable for up to 3 months. Further, full-field ERG showed clear light-evoked a and b waves in both treated and untreated eyes at 3 months post-transplantation. Labeled pre-induced hPBMCs were also observed in the contralateral optic nerve but not in the blood circulation, suggesting migration via the optic chiasm. Conclusion It may be possible to treat binocular eye diseases by unilateral stem cell injection. Graphical abstract |
| first_indexed | 2024-12-16T15:14:50Z |
| format | Article |
| id | doaj.art-3730b92a586343dfbdcb7618c0bfe440 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-16T15:14:50Z |
| publishDate | 2021-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-3730b92a586343dfbdcb7618c0bfe4402022-12-21T22:26:49ZengBMCStem Cell Research & Therapy1757-65122021-03-0112111310.1186/s13287-021-02180-5Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in miceJianfa Huang0Bikun Xian1Yuting Peng2Baozhu Zeng3Weihua Li4Zhiquan Li5Yaojue Xie6Minglei Zhao7Hening Zhang8Minyi Zhou9Huan Yu10Peixin Wu11Xing Liu12Bing Huang13State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen UniversityState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen UniversityState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen UniversityState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen UniversityState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen UniversityState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen UniversityState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen UniversityState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen UniversityState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen UniversityState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen UniversityState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen UniversityState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen UniversityState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen UniversityState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen UniversityAbstract Background Retina diseases may lead to blindness as they often afflict both eyes. Stem cell transplantation into the affected eye(s) is a promising therapeutic strategy for certain retinal diseases. Human peripheral blood mononuclear cells (hPBMCs) are a good source of stem cells, but it is unclear whether pre-induced hPBMCs can migrate from the injected eye to the contralateral eye for bilateral treatment. We examine the possibility of bilateral cell transplantation from unilateral cell injection. Methods One hundred and sixty-one 3-month-old retinal degeneration 1 (rd1) mice were divided randomly into 3 groups: an untreated group (n = 45), a control group receiving serum-free Dulbecco’s modified Eagle’s medium (DMEM) injection into the right subretina (n = 45), and a treatment group receiving injection of pre-induced hPBMCs into the right subretina (n = 71). Both eyes were examined by full-field electroretinogram (ERG), immunofluorescence, flow cytometry, and quantitative real-time polymerase chain reaction (qRT-PCR) at 1 and 3 months post-injection. Results At both 1 and 3 months post-injection, labeled pre-induced hPBMCs were observed in the retinal inner nuclear layer of the contralateral (left untreated) eye as well as the treated eye as evidenced by immunofluorescence staining for a human antigen. Flow cytometry of fluorescently label cells and qRT-PCR of hPBMCs genes confirmed that transplanted hPBMCs migrated from the treated to the contralateral untreated eye and remained viable for up to 3 months. Further, full-field ERG showed clear light-evoked a and b waves in both treated and untreated eyes at 3 months post-transplantation. Labeled pre-induced hPBMCs were also observed in the contralateral optic nerve but not in the blood circulation, suggesting migration via the optic chiasm. Conclusion It may be possible to treat binocular eye diseases by unilateral stem cell injection. Graphical abstracthttps://doi.org/10.1186/s13287-021-02180-5Human peripheral blood mononuclear cellsStem cellsCell therapyMigrationOcular diseases |
| spellingShingle | Jianfa Huang Bikun Xian Yuting Peng Baozhu Zeng Weihua Li Zhiquan Li Yaojue Xie Minglei Zhao Hening Zhang Minyi Zhou Huan Yu Peixin Wu Xing Liu Bing Huang Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice Stem Cell Research & Therapy Human peripheral blood mononuclear cells Stem cells Cell therapy Migration Ocular diseases |
| title | Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice |
| title_full | Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice |
| title_fullStr | Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice |
| title_full_unstemmed | Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice |
| title_short | Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice |
| title_sort | migration of pre induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice |
| topic | Human peripheral blood mononuclear cells Stem cells Cell therapy Migration Ocular diseases |
| url | https://doi.org/10.1186/s13287-021-02180-5 |
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